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LIN28B (human)

Overview LIN28B Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Interacts with ZCCHC11/TUT4. Might be negatively regulated by the microRNA let-7b. High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues. Belongs to the lin-28 family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Unknown function Cellular Component: mitochondrion; cytoplasm; nucleolus; nucleus Molecular Function: protein binding; DNA binding; zinc ion binding; RNA binding Biological Process: regulation of transcription, DNA-dependent; pre-microRNA processing; RNA 3'-end processing Reference #:  Q6ZN17 (UniProtKB) Alt. Names/Synonyms: CSDD2; FLJ16517; lin-28 homolog B (C. elegans); Lin-28.2; Lin-28B; LIN28B; LN28B; Protein lin-28 homolog B Gene Symbols: LIN28B Molecular weight: 27,084 Da Basal Isoelectric point: 9.15  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below LIN28B 4A4I - A/B=24-111 (human)

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S96-p	KSSKGLEsIRVTGPG
0	1		S105-p	RVTGPGGsPCLGSER
0	1		S203-p	GGGHGCTsPPFPQEA
0	1		S218-p	RAEISERsGRsPQEA
0	3		S221-p	ISERsGRsPQEASST
0	3		K240-a	APEEQSKkGPSVQKR

	mouse
S94	KSPKGLESIRVTGPG
S103	RVTGPGGSPCLGSER
-	gap
S215	KSEMAEHSDRsPQEV
S218-p	MAEHSDRsPQEVSST
K237	AIGEQNKKGPLIQKR

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