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Protein Page:
SLC3A2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SLC3A2 a transmembrane solute carrier protein that transports L-type amino acids and regulates intracellular calcium levels. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Disulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Colocalizes with cadherins. Interacts with FAM57A and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1. Expression is induced in resting peripheral blood T- lymphocytes following PHA stimulation. Expression increases at the time of maximal DNA synthesi, in fibroblasts stimulated to divide. Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid- trimester stage. Belongs to the SLC3A transporter family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: membrane; apical plasma membrane; cytoplasm; melanosome; plasma membrane; integral to membrane; nucleus; cell junction
Molecular Function: cation binding; protein binding; neutral amino acid transmembrane transporter activity; double-stranded RNA binding; catalytic activity; calcium:sodium antiporter activity
Biological Process: response to exogenous dsRNA; amino acid transport; calcium ion transport; carbohydrate metabolic process; ion transport; cell growth; blood coagulation; leukocyte migration; transmembrane transport; tryptophan transport
Reference #:  P08195 (UniProtKB)
Alt. Names/Synonyms: 4F2; 4F2 cell-surface antigen heavy chain; 4F2 heavy chain antigen; 4F2hc; 4T2HC; antigen defined by monoclonal antibody 4F2, heavy chain; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43; CD98; CD98 heavy chain; CD98HC; heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU1; monoclonal antibody 44D7; NACAE; SLC3A2; solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2; solute carrier family 3, member 2
Gene Symbols: SLC3A2
Molecular weight: 67,994 Da
Basal Isoelectric point: 4.89  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SLC3A2

Protein Structure Not Found.


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Sites Implicated In
cell adhesion, altered: S406‑p, S408‑p, S410‑p, S527‑p, S531‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S9-p ELQPPEAsIAVVSIP
0 9 S103-p AEVTGTMsQDtEVDM
0 2 T106-p TGTMsQDtEVDMkEV
1 1 K111-ub QDtEVDMkEVELNEL
1 3 K122-ub LNELEPEkQPMNAAs
0 1 S129-p kQPMNAAsGAAMsLA
0 7 S134-p AAsGAAMsLAGAEkN
0 12 K140-ub MsLAGAEkNGLVkIk
0 5 K145-ub AEkNGLVkIkVAEDE
0 96 K147-ub kNGLVkIkVAEDEAE
0 5 K160-ub AEAAAAAkFtGLskE
0 1 T162-p AAAAAkFtGLskEEL
0 9 S165-p AAkFtGLskEELLkV
0 76 K166-ub AkFtGLskEELLkVA
0 1 K166-sc AkFtGLskEELLkVA
0 3 K171-ub LskEELLkVAGsPGW
0 1 S175-p ELLkVAGsPGWVRtR
0 1 T181-p GsPGWVRtRWALLLL
0 2 K245-ub AGNLAGLkGRLDYLS
0 1 K245-sc AGNLAGLkGRLDYLS
0 1 K269-ub GPIHKNQkDDVAQTD
0 1 K269-sc GPIHKNQkDDVAQTD
0 1 K287-ub IDPNFGSkEDFDsLL
0 1 S292-p GSkEDFDsLLQsAkK
0 2 S296-p DFDsLLQsAkKKSIR
0 1 K298-ub DsLLQsAkKKSIRVI
0 1 K298-sc DsLLQsAkKKSIRVI
0 1 S317-p PNYRGENsWFstQVD
0 1 S320-p RGENsWFstQVDTVA
0 1 T321-p GENsWFstQVDTVAT
0 1 K329-ub QVDTVATkVKDALEF
0 1 K354-ub VRDIENLkDASSFLA
0 4 K368-ub AEWQNITkGFSEDRL
1 0 S406-p LLTSSYLsDsGsTGE
1 0 S408-p TSSYLsDsGsTGEHT
1 0 S410-p SYLsDsGsTGEHTkS
0 1 K416-ub GsTGEHTkSLVTQYL
0 1 S513-p NMTVKGQsEDPGsLL
0 1 S518-p GQsEDPGsLLsLFRR
0 1 S521-p EDPGsLLsLFRRLsD
1 0 S527-p LsLFRRLsDQRsKER
1 0 S531-p RRLsDQRsKERSLLH
0 1 S598-p AKADLLLsTQPGREE
0 2 S607-p QPGREEGsPLELERL
0 2 K615-ub PLELERLkLEPHEGL
  SLC3A2 iso3  
S9 ELQPPEASIAVVSIP
S41 GDDSGTMSQDTEVDM
T44 SGTMSQDTEVDMkEV
K49-ub QDTEVDMkEVELNEL
K60 LNELEPEKQPMNAAS
S67 KQPMNAASGAAMSLA
S72 AASGAAMSLAGAEKN
K78 MSLAGAEKNGLVKIK
K83 AEKNGLVKIKVAEDE
K85 KNGLVKIKVAEDEAE
K98 AEAAAAAKFTGLSKE
T100 AAAAAKFTGLSKEEL
S103 AAKFTGLSKEELLKV
K104 AKFTGLSKEELLKVA
K104 AKFTGLSKEELLKVA
K109 LSKEELLKVAGSPGW
S113 ELLKVAGSPGWVRTR
T119 GSPGWVRTRWALLLL
K183 AGNLAGLKGRLDYLS
K183 AGNLAGLKGRLDYLS
K207 GPIHKNQKDDVAQTD
K207 GPIHKNQKDDVAQTD
K225 IDPNFGSKEDFDSLL
S230 GSKEDFDSLLQSAKK
S234 DFDSLLQSAKKKSIR
K236 DSLLQSAKKKSIRVI
K236 DSLLQSAKKKSIRVI
S255 PNYRGENSWFSTQVD
S258 RGENSWFSTQVDTVA
T259 GENSWFSTQVDTVAT
K267 QVDTVATKVKDALEF
K292 VRDIENLKDASSFLA
K306 AEWQNITKGFSEDRL
S344 LLTSSYLSDSGSTGE
S346 TSSYLSDSGSTGEHT
S348 SYLSDSGSTGEHTKS
K354 GSTGEHTKSLVTQYL
S451 NMTVKGQSEDPGSLL
S456 GQSEDPGSLLSLFRR
S459 EDPGSLLSLFRRLSD
S465 LSLFRRLSDQRSKER
S469 RRLSDQRSKERSLLH
S536 AKADLLLSTQPGREE
S545 QPGREEGSPLELERL
K553 PLELERLKLEPHEGL
  SLC3A2 iso4  
S9 ELQPPEASIAVVSIP
S134 AGITGTMSQDTEVDM
T137 TGTMSQDTEVDMKEV
K142 QDTEVDMKEVELNEL
K153 LNELEPEKQPMNAAS
S160 KQPMNAASGAAMSLA
S165 AASGAAMSLAGAEKN
K171 MSLAGAEKNGLVKIK
K176 AEKNGLVKIKVAEDE
K178 KNGLVKIKVAEDEAE
K191 AEAAAAAKFTGLSKE
T193 AAAAAKFTGLSKEEL
S196 AAKFTGLSKEELLKV
K197 AKFTGLSKEELLKVA
K197 AKFTGLSKEELLKVA
K202 LSKEELLKVAGSPGW
S206 ELLKVAGSPGWVRTR
T212 GSPGWVRTRWALLLL
K276 AGNLAGLKGRLDYLS
K276 AGNLAGLKGRLDYLS
K300 GPIHKNQKDDVAQTD
K300 GPIHKNQKDDVAQTD
K318 IDPNFGSKEDFDSLL
S323 GSKEDFDSLLQSAKK
S327 DFDSLLQSAKKKSIR
K329 DSLLQSAKKKSIRVI
K329 DSLLQSAKKKSIRVI
S348 PNYRGENSWFSTQVD
S351 RGENSWFSTQVDTVA
T352 GENSWFSTQVDTVAT
K360 QVDTVATKVKDALEF
K385 VRDIENLKDASSFLA
K399 AEWQNITKGFSEDRL
S437 LLTSSYLSDSGSTGE
S439 TSSYLSDSGSTGEHT
S441 SYLSDSGSTGEHTKS
K447 GSTGEHTKSLVTQYL
S544 NMTVKGQSEDPGSLL
S549 GQSEDPGSLLSLFRR
S552 EDPGSLLSLFRRLSD
S558 LSLFRRLSDQRSKER
S562 RRLSDQRSKERSLLH
S629 AKADLLLSTQPGREE
S638 QPGREEGSPLELERL
K646 PLELERLKLEPHEGL
  mouse

 
- gap
S2-p ______MsQDTEVDM
T5 ___MsQDTEVDMKDV
K10 QDTEVDMKDVELNEL
K21 LNELEPEKQPMNAAD
D28 KQPMNAADGAAAGEk
- gap
K35-ub DGAAAGEkNGLVKIk
K40 GEkNGLVKIkVAEDE
K42-ub kNGLVKIkVAEDETE
K53-ub DETEAGVkFTGLskE
T55 TEAGVkFTGLskEEL
S58-p GVkFTGLskEELLKV
K59-ub VkFTGLskEELLKVA
K59 VkFTGLsKEELLKVA
K64 LskEELLKVAGSPGW
S68 ELLKVAGSPGWVRTR
T74 GSPGWVRTRWALLLL
K138 AGGIAGLKSHLEYLS
K138 AGGIAGLKSHLEYLS
K162 GPIHKNQKDEINETD
K162 GPIHKNQKDEINETD
Q180 INPTLGSQEDFKDLL
D185 GSQEDFKDLLQSAKK
S189 DFKDLLQSAKKKSIH
K191 KDLLQSAKKKSIHII
K191 KDLLQSAKKKSIHII
A210 PNYQGQNAWFLPAQA
L213 QGQNAWFLPAQADIV
A215 QNAWFLPAQADIVAT
K223 QADIVATKMKEALSS
M248 FRDVGKLMNAPLYLA
K262-ub AEWQNITkNLSEDRL
S300 LLTSSYLSNSTFTGE
S302 TSSYLSNSTFTGERT
F304 SYLSNSTFTGERTES
E310 TFTGERTESLVTRFL
N406 NMTVKGQNEDPGSLL
S411 GQNEDPGSLLTQFRR
T414 EDPGSLLTQFRRLSD
S420 LTQFRRLSDLRGKER
G424 RRLSDLRGKERSLLH
S491 ASAKLLLSTDSARQS
T503 RQSREEDTSLKLENL
S511 SLKLENLSLNPYEGL
  rat

 
- gap
S2-p ______MsQDtEVDM
T5-p ___MsQDtEVDMkDV
K10-ub QDtEVDMkDVELNEL
K21-ub LNELEPEkQPMNAAD
D28 kQPMNAADGAAAGEK
- gap
K35 DGAAAGEKNGLVKIK
K40 GEKNGLVKIKVAEDE
K42 KNGLVKIKVAEDEAE
K53 DEAEAGVKFTGLsKE
T55 AEAGVKFTGLsKEEL
S58-p GVKFTGLsKEELLKV
K59 VKFTGLsKEELLKVA
K59 VKFTGLsKEELLKVA
K64 LsKEELLKVAGSPGW
S68 ELLKVAGSPGWVRTR
T74 GSPGWVRTRWALLLL
K138 ARGIAGLKNHLEYLS
K138 ARGIAGLKNHLEYLS
K162 GPIHKNQKDEVNETD
K162 GPIHKNQKDEVNETD
Q180 IDPDLGSQEDFKDLL
D185 GSQEDFKDLLQSAKK
S189 DFKDLLQSAKKKSIH
K191 KDLLQSAKKKSIHII
K191 KDLLQSAKKKSIHII
- gap
L213 KGQNAWFLPPQADIV
P215 QNAWFLPPQADIVAT
K223 QADIVATKMKEALSS
A248 VRDVGKLANASLYLA
K262 AEWQNITKNFSEDRL
S300 LLTSSYLSQPVFTGE
P302 TSSYLSQPVFTGEHA
F304 SYLSQPVFTGEHAEL
E310 VFTGEHAELLVIKYL
N407 NMTVKGQNEDPGSLL
S412 GQNEDPGSLLTQFRR
T415 EDPGSLLTQFRRLSD
S421 LTQFRRLSDLRGKER
G425 RRLSDLRGKERSLLH
S492 ASANLLLSTDSTRLS
T504 RLSREEGTSLSLENL
S512 SLSLENLSLNPYEGL
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