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Protein Page:
KLF4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
KLF4 Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor; C2H2-type zinc finger protein
Cellular Component: transcription factor complex; nuclear chromatin; nucleolus; nucleus
Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity
Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein metabolic process; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; response to retinoic acid; negative regulation of protein kinase B signaling cascade; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade
Reference #:  O43474 (UniProtKB)
Alt. Names/Synonyms: endothelial Kruppel-like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; Gut-enriched krueppel-like factor; KLF4; Krueppel-like factor 4; Kruppel-like factor 4 (gut)
Gene Symbols: KLF4
Molecular weight: 54,671 Da
Basal Isoelectric point: 8.69  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

KLF4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K32-ub SGPAGREkTLRQAGA
0 2 T33 GPAGREkTLRQAGAP
1 0 S135 ASASSSSSPSSSGPA
0 1 G165 NDPGVAPGGTGGGLL
0 1 S234-p GKFVLKAsLSAPGSE
0 1 S244-p APGSEYGsPSVISVS
0 7 S254-p VISVSKGsPDGsHPV
0 1 S258-p SKGsPDGsHPVVVAP
2 0 K278-sm PRTCPKIkQEAVSSC
0 2 T316 RQLPSRTTPTLGLEE
0 2 G320 SRTTPTLGLEEVLSs
0 1 S327-p GLEEVLSsRDCHPAL
1 0 T431 RKRTATHTCDYAGCG
0 2 K448 YTKSSHLKAHLRTHT
1 0 S500 QKCDRAFSRSDHLAL
  mouse

 
K32 SGPAGREKtLRPAGA
T33-p GPAGREKtLRPAGAP
S132-p ASASSSSsPASSGPA
S162-p GDPGVAAsNTGGGLL
S231 GKFVLKASLTTPGSE
S241 TPGSEYSSPSVISVS
S251-p VISVSKGsPDGsHPV
S255-p SKGsPDGsHPVVVAP
K275-sm PRMCPKIkQEAVPSC
T320-p RQLPTRTtPTLsPEE
S324-p TRTtPTLsPEELLNS
S331 sPEELLNSRDCHPGL
T401 RKRTATHTCDYAGCG
K418-ub YTKSSHLkAHLRTHT
S470 QKCDRAFSRSDHLAL
  rat

 
K32 SGPAGREKTLRPAGA
T33 GPAGREKTLRPAGAP
S131 ASASSSSSPASSGPA
G161 GDPGVAAGNTGGGLL
S230 GKFVLKASLSTPGSE
S240 TPGSEYTSPSVISVS
S250-p VISVSKGsPDGSHPV
S254 SKGsPDGSHPVVVAP
K274 PRMCPKIKQEAVPSC
T319 RQLPTRTTPTLsPEE
S323-p TRTTPTLsPEELLNS
S330 sPEELLNSRDCHPGL
T400-p RKRTATHtCDYAGCG
K417 YTKSSHLKAHLRTHT
S469-p QKCDRAFsRSDHLAL
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