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RNF40 (human)

Overview RNF40 Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC. Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. Belongs to the BRE1 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system Cellular Component: neuron projection; cytoplasm; nucleolus; plasma membrane; nucleus; ubiquitin ligase complex Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; ubiquitin protein ligase binding; syntaxin-1 binding; protein complex binding; ubiquitin-protein ligase activity Biological Process: ubiquitin-dependent protein catabolic process; histone monoubiquitination; histone H2B ubiquitination Reference #:  O75150 (UniProtKB) Alt. Names/Synonyms: 95 kDa retinoblastoma protein binding protein; 95 kDa retinoblastoma-associated protein; BRE1 E3 ubiquitin ligase homolog B; BRE1-B; BRE1B; DKFZp686K191; E3 ubiquitin-protein ligase BRE1B; KIAA0661; MGC13051; Rb-associated protein; RBP95; RING finger protein 40; RNF40; STARING Gene Symbols: RNF40 Molecular weight: 113,650 Da Basal Isoelectric point: 5.93  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below RNF40

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	60		K20-a	GGSGPPEkkLSREEK
0	3		K21-a	GSGPPEkkLSREEKT
1	0		S114-p	ALLRCHEsQGELSSA
0	5		T127-p	SAPEAPGtQEGPTCD
0	1		K517-a	EVQAEIGkLRAQASG
0	4		S556-p	EGGPGPVstPDNRkE
0	3		T557-p	GGPGPVstPDNRkEM
0	2		K562-a	VstPDNRkEMAPVPG
0	1		T571-p	MAPVPGTtTTtTSVK
0	1		T574-p	VPGTtTTtTSVKKEE
0	2		P584	VKKEELVPSEEDFQG
0	2		S585	KKEELVPSEEDFQGI
0	2		T644-p	EKAKVEEtKRKESEL
0	1		Y676	MKLLLDMYKSAPKEQ
0	1		K686-a	APKEQRDkVQLMAAE
0	1		K743-u	QIEHLQRkLGATKQE
0	1		K795-m1	KLMSERIkANQIHKL
1	1		S819-p	EQVLGLKsQVDAQLL
1	1		S853-p	EKELTLRsQALELNK
0	1		Y976-p	FECVRGRyEARQRKC

	mouse
K20-a	GGSGPPEkKMNREEK
K21	GSGPPEkKMNREEKT
N114	ALLQCYENQRELSSG
C127	SGTEVPGCQEGLTRD
K517	EVQAEIGKLRAQASG
G556	DSGPGPGGTPDCKKE
T557	SGPGPGGTPDCKKEM
K562	GGTPDCKKEMALLAG
T571	MALLAGATSATSSIK
T574	LAGATSATSSIKKEE
S584-p	IKKEELVssEDDAQA
S585-p	KKEELVssEDDAQAL
A644	EKAKVEEAKRKESEL
Y676-p	MKLLLDMyKSAPKEQ
K686	APKEQRDKVQLMAAE
K743	QIEHLQRKLGATKQE
K795	KLMSERIKANQIHKL
S819	EQVLGLKSQVDAQLL
S853	EKELTLRSQALELNK
Y976	FECVRGRYEARQRKC

	rat
K20	GGSGPPEKKLNREEK
K21	GSGPPEKKLNREEKT
N114	ALLQCYENQRELSSS
C128	SGTEVPGCQEGLTRD
K518	EVQAEIGKLRAQASG
G557	DSGPGPGGTPDSKKE
T558	SGPGPGGTPDSKKEL
K563	GGTPDSKKELALVAG
T572	LALVAGATSVASSVK
A575	VAGATSVASSVKKEE
S585	VKKEELVSSEDDAQA
S586	KKEELVSSEDDAQAL
A645	EKAKAEEARRKESEL
Y677	MKLLLDMYKSAPKEQ
K687	APKEQRDKVQLMAAE
K744	QIEHLQRKLGATKQE
K796	KLMSERIKANQIHKL
S820	EQVLGLKSQVDAQLL
S854	EKELTLRSQALELNK
Y977	FECVRGRYEARQRKC

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