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Protein Page:
RNF40 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
RNF40 Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC. Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. Belongs to the BRE1 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; Ubiquitin conjugating system; EC 6.3.2.19; Ligase; Ubiquitin ligase
Cellular Component: neuron projection; nucleus; ubiquitin ligase complex
Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; ubiquitin protein ligase binding; syntaxin-1 binding; protein complex binding; ubiquitin-protein ligase activity
Biological Process: histone monoubiquitination; ubiquitin-dependent protein catabolic process; histone H2B ubiquitination
Reference #:  O75150 (UniProtKB)
Alt. Names/Synonyms: 95 kDa retinoblastoma protein binding protein; 95 kDa retinoblastoma-associated protein; BRE1 E3 ubiquitin ligase homolog B; BRE1-B; BRE1B; DKFZp686K191; E3 ubiquitin-protein ligase BRE1B; KIAA0661; MGC13051; Rb-associated protein; RBP95; RING finger protein 40; RNF40; STARING
Gene Symbols: RNF40
Molecular weight: 113,650 Da
Basal Isoelectric point: 5.93  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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RNF40

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 60 K20-a GGSGPPEkkLSREEK
0 3 K21-a GSGPPEkkLSREEKT
1 0 S114-p ALLRCHEsQGELSSA
0 5 T127-p SAPEAPGtQEGPTCD
0 4 S556-p EGGPGPVstPDNRkE
0 4 T557-p GGPGPVstPDNRkEM
0 2 K562-a VstPDNRkEMAPVPG
0 2 P584 VKKEELVPSEEDFQG
0 2 S585 KKEELVPSEEDFQGI
0 2 T644-p EKAKVEEtKRKESEL
0 1 Y676 MKLLLDMYKSAPKEQ
1 1 S819-p EQVLGLKsQVDAQLL
1 1 S853-p EKELTLRsQALELNK
  mouse

 
K20-a GGSGPPEkKMNREEK
K21 GSGPPEkKMNREEKT
N114 ALLQCYENQRELSSG
C127 SGTEVPGCQEGLTRD
G556 DSGPGPGGTPDCKKE
T557 SGPGPGGTPDCKKEM
K562 GGTPDCKKEMALLAG
S584-p IKKEELVssEDDAQA
S585-p KKEELVssEDDAQAL
A644 EKAKVEEAKRKESEL
Y676-p MKLLLDMyKSAPKEQ
S819 EQVLGLKSQVDAQLL
S853 EKELTLRSQALELNK
  rat

 
K20 GGSGPPEKKLNREEK
K21 GSGPPEKKLNREEKT
N114 ALLQCYENQRELSSS
C128 SGTEVPGCQEGLTRD
G557 DSGPGPGGTPDSKKE
T558 SGPGPGGTPDSKKEL
K563 GGTPDSKKELALVAG
S585 VKKEELVSSEDDAQA
S586 KKEELVSSEDDAQAL
A645 EKAKAEEARRKESEL
Y677 MKLLLDMYKSAPKEQ
S820 EQVLGLKSQVDAQLL
S854 EKELTLRSQALELNK
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