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Protein Page:
SLC12A3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SLC12A3 Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. Interacts with KLHL3. Predominant in kidney. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter
Cellular Component: membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; cytosol
Molecular Function: protein binding; transporter activity; sodium:chloride symporter activity
Biological Process: transport; sodium ion transport; ion transport; chloride transport; transmembrane transport
Reference #:  P55017 (UniProtKB)
Alt. Names/Synonyms: FLJ96318; Na-Cl symporter; NaCl electroneutral thiazide-sensitive cotransporter; NCCT; S12A3; SLC12A3; solute carrier family 12 (sodium/chloride transporters), member 3; Solute carrier family 12 member 3; thiazide-sensitive Na-Cl cotransporter; Thiazide-sensitive sodium-chloride cotransporter; TSC
Gene Symbols: SLC12A3
Molecular weight: 113,139 Da
Basal Isoelectric point: 8.1  Predict pI for various phosphorylation states
Select Structure to View Below

SLC12A3

Protein Structure Not Found.


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Sites Implicated In
activity, induced: T60‑p
molecular association, regulation: T55‑p, T60‑p, S73‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S39 SPPAAYDSSHPSHLt
0 1 S40 PPAAYDSSHPSHLtH
0 1 S43 AYDSSHPSHLtHSST
3 2 T46-p SSHPSHLtHSSTFCM
0 1 S49 PSHLtHSSTFCMRtF
3 1 T50 SHLtHSSTFCMRtFG
0 1 C52 LtHSSTFCMRtFGYN
16 2 T55-p SSTFCMRtFGYNtID
16 2 T60-p MRtFGYNtIDVVPtY
0 2 T66-p NtIDVVPtYEHyANs
0 1 Y70-p VVPtYEHyANstQPG
9 3 S73-p tYEHyANstQPGEPR
0 1 T74-p YEHyANstQPGEPRK
0 1 K81 tQPGEPRKVRPtLAD
0 2 T85-p EPRKVRPtLADLHsF
1 4 S91-p PtLADLHsFLKQEGR
0 1 T124 LVEGEAGTSSEKNPE
1 2 S126 EGEAGTSSEKNPEEP
0 1 K128 EAGTSSEKNPEEPVR
0 1 K140-ac PVRFGWVkGVMIRCM
0 1 K199 ISTNGKVKSGGTYFL
0 1 K706 WLNKRKIKAFYSDVI
0 1 K734-ac AAGLGRMkPNILVVG
0 1 K743-ac NILVVGFkKNWQSAH
0 1 S783-p MREGLNVsKMMQAHI
0 1 K828 IFQSEQGKKTIDIYW
0 1 K877 NRMDQERKAIISLLS
0 4 K885-ac AIISLLSkFRLGFHE
0 1 N902 ILPDINQNPRAEHTK
0 1 K909 NPRAEHTKRFEDMIA
0 1 K925 FRLNDGFKDEATVNE
0 1 K940 MRRDCPWKISDEEIT
0 1 K948 ISDEEITKNRVKSLR
  mouse

 
S37-p PPPAACDssQPsHLt
S38-p PPAACDssQPsHLtH
S41-p ACDssQPsHLtHGst
T44-p ssQPsHLtHGstLyM
S47-p PsHLtHGstLyMRtF
T48-p sHLtHGstLyMRtFG
Y50-p LtHGstLyMRtFGYN
T53-p GstLyMRtFGYNtID
T58-p MRtFGYNtIDVVPAY
A64 NtIDVVPAYEHYANs
Y68 VVPAYEHYANsALPG
S71-p AYEHYANsALPGEPR
A72 YEHYANsALPGEPRk
K79-ub ALPGEPRkVRPtLAD
T83-p EPRkVRPtLADLHsF
S89-p PtLADLHsFLKQEGS
T122-p LVEDETGtNsEkSPG
S124-p EDETGtNsEkSPGEP
K126-ub ETGtNsEkSPGEPVR
K138 PVRFGWVKGVMIRCM
K197-ub ISTNGKVkSGGTYFL
K704-ub WLNKRKIkAFYSDVI
K732 ASGLGRMKPNILVVG
K741 NILVVGFKRNWQSAH
S781 MREGLNVSEALQTHT
K809-ub IFQSEQGkKTIDIYW
K858-ub NRMDEERkAIISLLS
K866 AIISLLSKFRLGFHE
K883-ub VLPDINQkPQAEHTk
K890-ub kPQAEHTkRFEDMIA
K906-ub FRLNDGFkDEATVTE
K921-ub MRRDCPWkISDEEIN
K929-ub ISDEEINkNRIKSLR
  rat

 
N37 PPPAACDNSQPSHLT
S38 PPAACDNSQPSHLTH
S41 ACDNSQPSHLTHGSt
T44 NSQPSHLTHGStLYL
S47 PSHLTHGStLYLRtF
T48-p SHLTHGStLYLRtFG
Y50 LTHGStLYLRtFGYN
T53-p GStLYLRtFGYNtID
T58-p LRtFGYNtIDVVPAY
A64 NtIDVVPAYEHYANs
Y68 VVPAYEHYANsALPG
S71-p AYEHYANsALPGEPR
A72 YEHYANsALPGEPRK
K79 ALPGEPRKVRPTLAD
T83 EPRKVRPTLADLHSF
S89 PTLADLHSFLKQEGS
A122 LVEDETGANsEKSPG
S124-p EDETGANsEKSPGEP
K126 ETGANsEKSPGEPVR
K138 PVRFGWVKGVMIRCM
K197 ISTNGKVKSGGTYFL
K704 WLNKRKIKAFYSDVI
K732 ASGLGRMKPNILVVG
K741 NILVVGFKKNWQSAH
S781 MREGLNVSEALQTHT
K809 IFQSEQGKKTIDIYW
K858 NRMDEERKAIISLLS
K866 AIISLLSKFRLGFHE
K883 VLPDINQKPQVEHTK
K890 KPQVEHTKRFEDMIA
K906 FRLNDGFKDEATVAE
K921 MRRDYPWKISDEEIN
K929 ISDEEINKNRIKSLR
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