Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
LEF-1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LEF-1 a transcription factor of the TCF/LEF family. Participates in the Wnt signaling pathway and regulates T-cell receptor alpha-C enhancer function. Activates transcription of target genes in the presence of CTNNB1 and EP300. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. Deletions in LEF1 have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Four human isoforms produced by alternative promoter usage and alternative splicing have been described. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; estrogen receptor activity; DNA binding; histone binding; sequence-specific DNA binding; gamma-catenin binding; caspase inhibitor activity; beta-catenin binding; estrogen receptor binding; chromatin binding; DNA bending activity; transcription factor activity; transcription factor binding
Biological Process: radial glial cell differentiation in the forebrain; hypothalamus development; positive regulation of transcription, DNA-dependent; T-helper 1 cell differentiation; Wnt receptor signaling pathway through beta-catenin; forebrain neuroblast division; forebrain neuron differentiation; BMP signaling pathway; negative regulation of interleukin-13 production; neural crest cell migration; embryonic limb morphogenesis; regulation of striated muscle development; somitogenesis; dentate gyrus development; sensory perception of taste; negative regulation of striated muscle development; positive regulation of cell growth; patterning of blood vessels; negative regulation of interleukin-5 production; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; transcription from RNA polymerase II promoter; B cell proliferation; negative regulation of interleukin-4 production; tongue development; paraxial mesoderm formation; positive regulation of granulocyte differentiation; alpha-beta T cell differentiation; negative regulation of caspase activity; palate development; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; regulation of cell-cell adhesion; mammary gland development; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; muscle fiber development; Wnt receptor signaling pathway; neutrophil differentiation; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; osteoblast differentiation; formation of radial glial scaffolds; T cell receptor V(D)J recombination; epithelial to mesenchymal transition; negative regulation of cell-cell adhesion; sprouting angiogenesis; eye pigmentation; positive regulation of cell migration
Reference #:  Q9UJU2 (UniProtKB)
Alt. Names/Synonyms: DKFZp586H0919; FLJ46390; LEF-1; LEF1; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; TCF1ALPHA
Gene Symbols: LEF1
Molecular weight: 44,201 Da
Basal Isoelectric point: 6.9  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/├č-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

LEF-1

Protein Structure Not Found.


STRING  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
transcription, induced: S42‑p, S61‑p
transcription, inhibited: T155‑p, S166‑p
molecular association, regulation: S42‑p, S61‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 4 S42-p EKIFAEIsHPEEEGD
1 0 S61-p KSSLVNEsEIIPASN
0 1 S79-p VARQAQTsQEPyHDK
0 4 Y83-p AQTsQEPyHDKAREH
0 1 K95-ac REHPDDGkHPDGGLy
0 4 Y102-p kHPDGGLyNKGPSYS
1 0 Y114-p SYSSYSGyIMMPNMN
0 3 S132-p YMSNGSLsPPIPRTS
2 10 T155-p SHAVHPLtPLItysD
0 1 T159-p HPLtPLItysDEHFs
0 2 Y160-p PLtPLItysDEHFsP
0 1 S161-p LtPLItysDEHFsPG
1 1 S166-p tysDEHFsPGSHPSH
0 2 T195-p PPAPDIPtFyPLsPG
0 1 Y197-p APDIPtFyPLsPGGV
0 18 S200-p IPtFyPLsPGGVGQI
0 23 T208-p PGGVGQItPPLGWQG
0 1 S238-p SSLSVDTsMsRFSHH
0 2 S240-p LSVDTsMsRFSHHMI
0 1 T265 IPHPAIVTPQVKQEH
1 0 K269 AIVTPQVKQEHPHTD
0 1 K295-ub QRKEQEPkRPHIKkP
0 1 K301-ub PkRPHIKkPLNAFML
0 3 K324-ub VVAECTLkESAAINQ
0 8 K347-ub LSREEQAkYYELARK
0 12 Y363-p RQLHMQLyPGWSARD
0 4 K382-ub KKKRKREkLQEsASG
0 2 S386-p KREkLQEsASGtGPR
0 2 T390-p LQEsASGtGPRMTAA
0 1 - gap
  LEF-1 iso6  
S42 EKIFAEISHPEEEGD
S61 KSSLVNESEIIPASN
S79 VARQAQTSQEPYHDK
Y83 AQTSQEPYHDKAREH
K95 REHPDDGKHPDGGLY
Y102 KHPDGGLYNKGPSYS
Y114 SYSSYSGYIMMPNMN
S132 YMSNGSLSPPIPRTS
T155 SHAVHPLTPLITYSD
T159 HPLTPLITYSDEHFS
Y160 PLTPLITYSDEHFSP
S161 LTPLITYSDEHFSPG
S166 TYSDEHFSPGSHPSH
T195 PPAPDIPTFYPLsPG
Y197 APDIPTFYPLsPGGV
S200-p IPTFYPLsPGGVGQI
T208-p PGGVGQItPPLGWFS
- gap
- gap
T237-p IPHPAIVtPQVKQEH
K241 AIVtPQVKQEHPHTD
K267 QRKEQEPKRPHIKKP
K273 PKRPHIKKPLNAFML
K296 VVAECTLKESAAINQ
K319 LSREEQAKYYELARK
Y335 RQLHMQLYPGWSARD
K354 KKKRKREKLQESASG
S358 KREKLQESASGGkRS
- gap
K363-ac QESASGGkRSSFPTC
  mouse

► Hide Isoforms
 
S40 EKIFAEISHPEEEGD
S59 KSSLVNESEIIPASN
S77 VVRQAPSSQEPYHDK
Y81 APSSQEPYHDKAREH
K93 REHPDEGKHPDGGLY
Y100 KHPDGGLYNKGPSYS
Y112 SYSSYSGYIMMPNMN
S130 YMSNGSLSPPIPRTS
T153 SHAVHPLTPLITYSD
T157 HPLTPLITYSDEHFS
Y158 PLTPLITYSDEHFSP
S159 LTPLITYSDEHFSPG
S164 TYSDEHFSPGSHPSH
T193 PPAPEIPTFYPLSPG
Y195 APEIPTFYPLSPGGV
S198 IPTFYPLSPGGVGQI
T206 PGGVGQITPPIGWQG
S236 SSLSGDTSMsRFSHH
S238-p LSGDTSMsRFSHHMI
T263 IPHPAIVTPQVkQEH
K267-sm AIVTPQVkQEHPHTD
K293 QRKEQEPKRPHIKKP
K299 PKRPHIKKPLNAFML
K322 VVAECTLKESAAINQ
K345 LSREEQAKYYELARK
Y361 RQLHMQLYPGWSARD
K380 KKKRKREKLQESTSG
S384 KREKLQESTSGTGPR
T388 LQESTSGTGPRMTAA
- gap
  LEF-1 iso3  
S40 EKIFAEISHPEEEGD
S59 KSSLVNESEIIPASN
S77 VVRQAPSSQEPYHDK
Y81 APSSQEPYHDKAREH
K93 REHPDEGKHPDGGLY
Y100 KHPDGGLYNKGPSYS
Y112 SYSSYSGYIMMPNMN
S130 YMSNGSLSPPIPRTS
T153 SHAVHPLTPLITYSD
T157 HPLTPLITYSDEHFS
Y158 PLTPLITYSDEHFSP
S159 LTPLITYSDEHFSPG
S164 TYSDEHFSPGSHPSH
T193 PPAPEIPTFYPLSPG
Y195 APEIPTFYPLSPGGV
S198 IPTFYPLSPGGVGQI
T206 PGGVGQITPPIGWFS
- gap
- gap
T235 IPHPAIVTPQVKQEH
K239 AIVTPQVKQEHPHTD
K265 QRKEQEPKRPHIKKP
K271 PKRPHIKKPLNAFML
K294 VVAECTLKESAAINQ
K317 LSREEQAKYYELARK
Y333 RQLHMQLYPGWSARD
K352 KKKRKREKLQEsTSG
S356-p KREKLQEsTSGGKRS
- gap
K361 QEsTSGGKRSSFPTC
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.