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Protein Page:
Smad3 (mouse)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; DNA binding protein; Transcription factor
Cellular Component: transcription factor complex; protein complex; cytoplasm; plasma membrane; nuclear inner membrane; intracellular; nucleus; receptor complex
Molecular Function: ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; metal ion binding; beta-catenin binding; protein kinase binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; collagen binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transcription factor activity; transforming growth factor beta receptor binding
Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; positive regulation of stress fiber formation; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; endoderm development; negative regulation of apoptosis; transcription from RNA polymerase II promoter; evasion of host defenses by virus; T cell activation; negative regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell proliferation; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; regulation of transcription, DNA-dependent; positive regulation of focal adhesion formation; transport; positive regulation of transforming growth factor-beta3 production; positive regulation of transcription factor import into nucleus; thyroid gland development; heart looping; skeletal development; caspase activation; intercellular junction assembly and maintenance; regulation of immune response; regulation of transforming growth factor-beta2 production; protein stabilization; regulation of binding; in utero embryonic development; liver development; positive regulation of bone mineralization; SMAD protein complex assembly; osteoblast differentiation; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; immune response; negative regulation of cell growth; positive regulation of cell migration
Reference #:  Q8BUN5 (UniProtKB)
Alt. Names/Synonyms: AU022421; MAD homolog 3; MAD homolog 3 (Drosophila); Mad3; Madh3; mMad3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; OTTMUSP00000023917; SMAD 3; SMAD family member 3; Smad3
Gene Symbols: Smad3
Molecular weight: 48,081 Da
Basal Isoelectric point: 6.73  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  ESC Pluripotency and Differentiation  |  G1/S Checkpoint  |  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Smad3

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
5 2 T8 MSSILPFTPPIVKRL
0 5 K19 VKRLLGWKKGEQNGQ
0 2 T132-p YHYQRVEtPVLPPVL
10 0 T179 PQSNIPETPPPGYLS
11 0 S204 NHSMDAGSPNLSPNP
11 0 S208 DAGSPNLSPNPMSPA
8 0 S213 NLSPNPMSPAHNNLD
2 0 S309-p EVFAECLsDSAIFVQ
1 1 K378 TIRMSFVKGWGAEYR
3 0 T388-p GAEYRRQtVTSTPCW
1 2 S416 KVLTQMGSPSIRCSs
2 0 S418 LTQMGSPSIRCSsVs
1 0 S422 GSPSIRCSsVs____
18 5 S423-p SPSIRCSsVs_____
18 5 S425-p SIRCSsVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
  human

 
T8-p MSSILPFtPPIVKRL
K19-a VKRLLGWkKGEQNGQ
T132-p YHYQRVEtPVLPPVL
T179-p PQSNIPEtPPPGYLS
S204-p NHSMDAGsPNLsPNP
S208-p DAGsPNLsPNPMsPA
S213-p NLsPNPMsPAHNNLD
S309-p EVFAECLsDSAIFVQ
K378-a TIRMSFVkGWGAEYR
T388-p GAEYRRQtVTSTPCW
S416-p KVLTQMGsPsIRCss
S418-p LTQMGsPsIRCssVs
S422-p GsPsIRCssVs____
S423-p sPsIRCssVs_____
S425-p sIRCssVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
  rat

 
T8 MSSILPFTPPIVKRL
K19 VKRLLGWKKGEQNGQ
T132 YHYQRVETPVLPPVL
T179 PQSNIPETPPPGYLS
S204 NHSMDAGSPNLSPNP
S208 DAGSPNLSPNPMSPA
S213 NLSPNPMSPAHNNLD
S309 EVFAECLSDSAIFVQ
K378 TIRMSFVKGWGAEYR
T388 GAEYRRQTVTSTPCW
S416 KVLTQMGSPSIRCSs
S418 LTQMGSPSIRCSsVs
S422 GSPSIRCSsVs____
S423-p SPSIRCSsVs_____
S425-p SIRCSsVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
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