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Protein Page:
NANOG (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NANOG Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor; Cell development/differentiation
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin binding; transcription corepressor activity; transcription factor activity
Biological Process: response to retinoic acid; transcription, DNA-dependent; somatic stem cell maintenance; stem cell division; multicellular organismal development; endodermal cell fate specification; positive regulation of transcription, DNA-dependent; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; stem cell differentiation; positive regulation of mitotic cell cycle; mesodermal cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of cell fate commitment; response to organic substance; cell proliferation; negative regulation of cell differentiation; embryonic development; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of endodermal cell fate specification; positive regulation of transcription from RNA polymerase II promoter
Reference #:  Q80Z64 (UniProtKB)
Alt. Names/Synonyms: 2410002E02Rik; early embryo specific expression NK family; ecat4; ENK; homeobox protein NANOG; homeobox transcription factor Nanog; Nanog; Nanog homeobox; Q5TM83; Q80Z64; Q80Z64-2
Gene Symbols: Nanog
Molecular weight: 34,194 Da
Basal Isoelectric point: 6.32  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NANOG

Protein Structure Not Found.


STRING  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  UniProtKB  |  Entrez-Gene  |  GenPept


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 0 Y35 AVFHPENYSCLQGSA
1 0 A50 TEMLCTEAASPRPSS
1 0 S52 MLCTEAASPRPSSED
1 0 S56 EAASPRPSSEDLPLQ
1 0 S57 AASPRPSSEDLPLQG
1 0 S65 EDLPLQGSPDSSTSP
1 0 T70 QGSPDSSTSPKQKLS
1 0 S71 GSPDSSTSPKQKLSs
1 1 S78-p SPKQKLSsPEADKGP
2 0 P79 PKQKLSsPEADKGPE
0 1 Y120 DRFQKQKYLSLQQMQ
1 1 S136 LSSILNLSYkQVKTW
0 2 K138-ub SILNLSYkQVKTWFQ
0 2 K169-ub TSNGLIQkGSAPVEY
1 0 Y176 kGSAPVEYPSIHCSY
1 0 T202 LSMWGSQTWTNPTWS
1 0 S266 YIQLQQNSSASDLEV
1 0 S281 NLEATRESHAHFSTP
0 1 T287 ESHAHFSTPQALELF
0 1 A290 AHFSTPQALELFLNY
  human

 
Y35-p ICGPEENyPSLQMSS
T50-p AEMPHTEtVsPLPss
S52-p MPHTEtVsPLPssMD
S56-p EtVsPLPssMDLLIQ
S57-p tVsPLPssMDLLIQD
S65-p MDLLIQDsPDSStsP
T70-p QDsPDSStsPKGKQP
S71-p DsPDSStsPKGKQPt
T78-p sPKGKQPtsAEKSVA
S79-p PKGKQPtsAEKSVAK
Y119 DRFQRQKYLSLQQMQ
S135-p LSNILNLsYKQVKTW
K137 NILNLsYKQVKTWFQ
K168 NSNGVTQKASAPTyP
Y174-p QKASAPTyPSLYSSY
T200-p LPMWSNQtWNNSTWS
S258-p CMQFQPNsPASDLEA
T280-p GLNVIQQtTRYFStP
T286-p QtTRYFStPQtMDLF
T289-p RYFStPQtMDLFLNY
  rat

 
Y36 VHLPEENYSCLQVSA
T51 TEMLCTETASPPPSS
S53 MLCTETASPPPSSGD
S57 ETASPPPSSGDLPLQ
S58 TASPPPSSGDLPLQD
S66 GDLPLQDSPDSSSNP
S71 QDSPDSSSNPKLKLS
N72 DSPDSSSNPKLKLSG
G79 NPKLKLSGPEADEGP
P80 PKLKLSGPEADEGPE
Y122-p DRFQRQRyLSLQQMQ
S138-p LSTILNLsYKQVKTW
K140 TILNLsYKQVKTWFQ
K171 TSNGLTQKGSAPVEY
Y178 KGSAPVEYPSIHCSY
T204 LPVWGSQTWTNPTWN
F273 YVPLQQNFSASDLEA
S288 NLEATRESQAHFSTP
T294 ESQAHFSTPQALELF
A297 AHFSTPQALELFLNY
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