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Protein Page:
UBE2S (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
UBE2S Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL. Belongs to the ubiquitin-conjugating enzyme family. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin conjugating system; Ligase; EC 6.3.2.19; Ubiquitin ligase
Cellular Component: anaphase-promoting complex
Molecular Function: acid-amino acid ligase activity; ubiquitin-protein ligase activity; ATP binding
Biological Process: anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; protein modification process; exit from mitosis
Reference #:  Q16763 (UniProtKB)
Alt. Names/Synonyms: E2-EPF; E2-EPF5; E2EPF; EPF5; UBE2S; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2S; Ubiquitin-protein ligase S
Gene Symbols: UBE2S
Molecular weight: 23,845 Da
Basal Isoelectric point: 8.45  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

UBE2S

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 3 K18-ub HIIRLVYkEVTTLTA
0 8 K68-ac RMKLLLGkDFPAsPP
0 2 K68-ub RMKLLLGkDFPAsPP
0 12 S73-p LGkDFPAsPPKGyFL
0 1 Y78-p PAsPPKGyFLtKIFH
0 1 T81-p PPKGyFLtKIFHPNV
0 22 K100-ub EICVNVLkRDWTAEL
0 1 K117-ub RHVLLTIkCLLIHPN
0 2 S173-p EAGRALAsGTEASSt
0 1 E176 RALAsGTEASStDPG
0 1 S178 LAsGTEASStDPGAP
0 1 S179 AsGTEASStDPGAPG
0 1 T180-p sGTEASStDPGAPGG
0 6 K197-ub GAEGPMAkkHAGERD
0 2 K198-ub AEGPMAkkHAGERDK
  mouse

 
K18 HIIRLVYKEVTTLTA
K68 RMKLLLGKDFPASPP
K68 RMKLLLGKDFPASPP
S73 LGKDFPASPPKGYFL
Y78 PASPPKGYFLTKIFH
T81 PPKGYFLTKIFHPNV
K100-ub EICVNVLkRDWTAEL
K117 RHVLLTIKCLLIHPN
S174-p EATQDLAsGAsAssA
S177-p QDLAsGAsAssADPM
S179-p LAsGAsAssADPMIP
S180-p AsGAsAssADPMIPG
A181 sGAsAssADPMIPGV
K198 GAEGPMAKKHAGERD
K199 AEGPMAKKHAGERDK
  rat

 
K18 HIIRLVYKEVTTLTA
K68 RMKLLLGKDFPASPP
K68 RMKLLLGKDFPASPP
S73 LGKDFPASPPKGYFL
Y78 PASPPKGYFLTKIFH
T81 PPKGYFLTKIFHPNV
K100 EICVNVLKRDWTAEL
K117 RHVLLTIKCLLIHPN
S174 EASRDLASGASASST
S177 RDLASGASASSTDPM
S179 LASGASASSTDPMTP
S180 ASGASASSTDPMTPG
T181 SGASASSTDPMTPGV
K198 GAEGPMAKKHAGERD
K199 AEGPMAKKHAGERDK
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