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Protein Page:
MED12 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MED12 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Cellular Component: nucleoplasm; Srb-mediator complex; nucleus
Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; beta-catenin binding; transcription cofactor activity; thyroid hormone receptor binding; chromatin binding; receptor activity
Biological Process: steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Schwann cell development; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; heart development; positive regulation of transcription, DNA-dependent; stem cell maintenance; Wnt receptor signaling pathway through beta-catenin; androgen receptor signaling pathway; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; gene expression; oligodendrocyte development
Reference #:  Q93074 (UniProtKB)
Alt. Names/Synonyms: Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; CAGH45; FGS1; HOPA; human opposite paired; KIAA0192; MED12; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OKS; OPA-containing protein; OPA1; Thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; TNRC11; TRAP230; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein
Gene Symbols: MED12
Molecular weight: 243,081 Da
Basal Isoelectric point: 6.63  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MED12

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 K80-ub FSSIIAEkLRCNTLP
0 1 Y166-p LIKMTCAyYAAISET
0 1 T302-p RRLAYFCtRRLALQL
0 1 S471-p DSHSFERsDFsNsLD
0 1 S474-p SFERsDFsNsLDsLC
0 1 S476-p ERsDFsNsLDsLCNR
0 1 S479-p DFsNsLDsLCNRIFG
0 28 S635-p APGPRPPsPFDDPAD
0 2 A641 PsPFDDPADDPEHKE
0 1 S665-p EDPGLSEsMDIDPSS
0 7 S698-p MPCEGKGsPsPEKPD
0 6 S700-p CEGKGsPsPEKPDVE
0 1 K780-ub KITKDILkVLNRKGT
0 1 K814 LGGEDGQKRRRNRPE
0 1 K988 SCSHLKNKFGELFSD
0 1 K999-ac LFSDFCSkVKNTIYC
0 1 K999 LFSDFCSKVKNTIYC
0 1 K1194 PCQSDGNKPTVGIRS
0 5 S1258-p EEEGGGGsGGRRQGG
0 2 S1269-p RQGGRNIsVETASLD
0 1 K1534-ub QLMHEALkLRLNLVG
0 1 K1761-ac PTLLEPEkKAPEPPK
0 1 K1761-ub PTLLEPEkKAPEPPK
0 1 S1778-p KPGAAPPstEERKKK
0 1 T1779-p PGAAPPstEERKKKS
0 7 K1798-ac KRSQPATkTEDYGMG
0 1 R1854 PLPAGGPRVDPYrPV
0 1 R1859-m2 GPRVDPYrPVrLPMQ
0 1 R1862-m2 VDPYrPVrLPMQKLP
0 11 R1899-m2 SYKTSVYrQQQPAVP
0 1 R1899 SYKTSVYRQQQPAVP
0 1 R1910 PAVPQGQRLrQQLQQ
0 1 R1910 PAVPQGQRLrQQLQQ
0 3 R1912-m2 VPQGQRLrQQLQQSQ
0 1 R1994-m2 PTRHLQQrPSGYVHQ
0 1 R2015-m2 HGLTSTQrFSHQTLQ
0 2 S2163-p RQLQQQLsNTQPQPS
  mouse

 
K80-ub FSSIIAEkLRCNTLS
Y166 LIKMTCAYYAAMSET
T303 RRLAYFCTRRLALQL
S472 DSHSFERSDFSNSLD
S475 SFERSDFSNSLDSLC
S477 ERSDFSNSLDSLCNR
S480 DFSNSLDSLCNRIFG
S636-p APGPRPPsPFDDPtD
T642-p PsPFDDPtDDPERKE
S666 EDPGLSESMDIDPSS
S699-p MPCEGKGsPsPEKPD
S701-p CEGKGsPsPEKPDVE
K781 KITKDILKVLNRKGT
K815-ub LGGEDGQkRRRNRPE
K989-ub SCSHLKSkFGELFSD
K1000 LFSDFCSKVKNTIYC
K1000-ub LFSDFCSkVKNTIYC
K1195-ub PCQSDGNkPTVGIRS
S1259 EEEGGGGSSGRRQGG
S1270 RQGGRNISVETASLD
K1535 QLMHEALKLRLNLVG
K1763 PALLEPEKKAPEPPK
K1763 PALLEPEKKAPEPPK
S1780 KPGAAPPSTEERKKK
T1781 PGAAPPSTEERKKKS
K1800 KRSQPATKNEDYGMG
R1856-m2 PLPAGGPrVDPYrPV
R1861-m2 GPrVDPYrPVrLPMQ
R1864-m2 VDPYrPVrLPMQKLP
R1901-m2 SYKTSVYrQQQPTVP
R1901-m1 SYKTSVYrQQQPTVP
R1912-m2 PTVPQGQrLrQQLQA
R1912-m1 PTVPQGQrLrQQLQA
R1914-m2 VPQGQrLrQQLQAKI
R1999 PTRHLQQRPSGYVHQ
R2020 HGLTSTQRFSHQTLQ
S2176 RQLQQQLSNTQPQPS
  rat

 
K80 FSSIIAEKLRCNTLS
Y166 LIKMTCAYYAAMSET
T303 RRLAYFCTRRLALQL
S472 DSHSFERSDFSNSLD
S475 SFERSDFSNSLDSLC
S477 ERSDFSNSLDSLCNR
S480 DFSNSLDSLCNRIFG
S636 TPGPRPPSPFDDPAD
A642 PSPFDDPADDPERKE
S666 EDPGLSESMDIDPSS
S699 MPCEGKGSPSPEKPD
S701 CEGKGSPSPEKPDVE
K781 KITKDILKVLNRKGT
K815 LGGEDGQKRRRNRPE
K989 SCSHLKSKFGELFSD
K1000 LFSDFCSKVKNTIYC
K1000 LFSDFCSKVKNTIYC
K1195 PCQSDGNKPTVGIRS
S1259 EEEGGGGSSGRRQGG
S1270 RQGGRNISVETASLD
K1535 QLMHEALKLRLNLVG
K1763 PALLEPEKKAPEPPK
K1763 PALLEPEKKAPEPPK
S1780 KPGTAPPSTEERKKK
T1781 PGTAPPSTEERKKKS
K1800 KRSQPATKSEDYGMG
R1856 PLPAGGPRVDPYRPV
R1861 GPRVDPYRPVRLPMQ
R1864 VDPYRPVRLPMQKLP
R1901 SYKTSVYRQQQPTVP
R1901 SYKTSVYRQQQPTVP
R1912 PTVPQGQRLRQQLQQ
R1912 PTVPQGQRLRQQLQQ
R1914 VPQGQRLRQQLQQSQ
R1996 PTRHLQQRPSGYVHQ
R2017 HGLTSTQRFSHQTLQ
S2173 RQLQQQLSNTQPQPS
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