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Protein Page:
Cofilin-1 (mouse)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Cofilin-1 a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein; Motility/polarity/chemotaxis
Cellular Component: extracellular space; cortical actin cytoskeleton; leading edge; intercellular junction; actin cytoskeleton; cytoskeleton; cell projection; membrane; lamellipodium; cytoplasm; plasma membrane; intracellular; nucleus
Molecular Function: actin binding
Biological Process: actin filament depolymerization; positive regulation of actin filament depolymerization; actin filament organization; cytoskeleton organization and biogenesis; negative regulation of cell size; response to amino acid stimulus; protein amino acid phosphorylation; neural fold formation; cell projection organization and biogenesis; cytokinesis after mitosis; establishment of cell polarity; protein import into nucleus; regulation of cell morphogenesis; cell motility; neural crest cell migration
Reference #:  P18760 (UniProtKB)
Alt. Names/Synonyms: AA959946; Cfl1; Cof; COF1; cofilin 1, non-muscle; Cofilin, non-muscle isoform; Cofilin-1
Gene Symbols: Cfl1
Molecular weight: 18,560 Da
Basal Isoelectric point: 8.22  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Microtubule Dynamics  |  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Cofilin-1

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  Source  |  UCSD-Nature  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
cytoskeletal reorganization: S3‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
53 35 S3-p _____MAsGVAVsDG
0 2 S8-p MAsGVAVsDGVIKVF
0 4 K13 AVsDGVIKVFNDMkV
0 2 K13 AVsDGVIKVFNDMkV
0 4 K19 IKVFNDMKVRKSstP
0 12 K19-u IKVFNDMkVRKSstP
0 5 K22 FNDMkVRKSstPEEV
1 2 S23 NDMkVRKSstPEEVK
1 12 S24-p DMkVRKSstPEEVKK
0 16 T25-p MkVRKSstPEEVKKR
0 3 K30 SstPEEVKKRkKAVL
0 3 K33-a PEEVKKRkKAVLFCL
0 6 S41-p KAVLFCLsEDkkNII
0 44 K44-a LFCLsEDkkNIILEE
0 11 K44-u LFCLsEDkkNIILEE
0 4 K45 FCLsEDkKNIILEEG
0 7 K45-u FCLsEDkkNIILEEG
0 6 K53 NIILEEGKEILVGDV
1 428 Y68-p GQTVDDPyTTFVkML
0 9 T70 TVDDPyTTFVkMLPD
0 10 K73-u DPyTTFVkMLPDkDC
0 1 K73 DPyTTFVKMLPDkDC
0 2 K78-u FVkMLPDkDCRYALy
0 2 K78 FVkMLPDKDCRYALy
0 46 Y82 LPDkDCRYALyDAty
0 287 Y85-p kDCRYALyDAtyEtk
0 55 T88-p RYALyDAtyEtkESk
0 545 Y89-p YALyDAtyEtkESkk
0 7 T91-p LyDAtyEtkESkkED
0 99 K92-u yDAtyEtkESkkEDL
0 8 K95-u tyEtkESkkEDLVFI
0 8 K96-a yEtkESkkEDLVFIF
0 73 K112-u APENAPLkSkMIyAS
0 57 K114-u ENAPLkSkMIyASSk
0 189 Y117-p PLkSkMIyASSkDAI
0 3 K121 kMIyASSKDAIKKKL
0 5 K121-u kMIyASSkDAIKKKL
1 8 K126 SSkDAIKKKLTGIKH
1 8 K127 SkDAIKKKLTGIKHE
1 17 K132 KKKLTGIKHELQANC
0 9 K132 KKKLTGIKHELQANC
0 1942 Y140-p HELQANCyEEVkDRC
0 15 K144-a ANCyEEVkDRCtLAE
0 100 K144-u ANCyEEVkDRCtLAE
0 1 T148-p EEVkDRCtLAEKLGG
0 11 S156-p LAEKLGGsAVISLEG
0 26 K164-u AVISLEGkPL_____
3311 : Phospho-Cofilin (Ser3) Antibody
3313 : Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
  human

 
S3-p _____MAsGVAVsDG
S8-p MAsGVAVsDGVIkVF
K13-a AVsDGVIkVFNDMkV
K13-u AVsDGVIkVFNDMkV
K19-a IkVFNDMkVRksstP
K19-u IkVFNDMkVRksstP
K22-u FNDMkVRksstPEEV
S23-p NDMkVRksstPEEVk
S24-p DMkVRksstPEEVkK
T25-p MkVRksstPEEVkKR
K30-a sstPEEVkKRkKAVL
K33-a PEEVkKRkKAVLFCL
S41-p KAVLFCLsEDkkNII
K44-a LFCLsEDkkNIILEE
K44-u LFCLsEDkkNIILEE
K45-a FCLsEDkkNIILEEG
K45-u FCLsEDkkNIILEEG
K53-u NIILEEGkEILVGDV
Y68-p GQTVDDPyAtFVkML
T70-p TVDDPyAtFVkMLPD
K73-u DPyAtFVkMLPDkDC
K73-a DPyAtFVkMLPDkDC
K78-u FVkMLPDkDCRyALy
K78-a FVkMLPDkDCRyALy
Y82-p LPDkDCRyALyDAty
Y85-p kDCRyALyDAtyEtk
T88-p RyALyDAtyEtkESk
Y89-p yALyDAtyEtkESkk
T91-p LyDAtyEtkESkkED
K92-u yDAtyEtkESkkEDL
K95-u tyEtkESkkEDLVFI
K96-a yEtkESkkEDLVFIF
K112-u APESAPLkSkMIyAS
K114-u ESAPLkSkMIyASSk
Y117-p PLkSkMIyASSkDAI
K121-a kMIyASSkDAIKkkL
K121-u kMIyASSkDAIKkkL
K126-a SSkDAIKkkLTGIkH
K127-a SkDAIKkkLTGIkHE
K132-a KkkLTGIkHELQANC
K132-u KkkLTGIkHELQANC
Y140-p HELQANCyEEVkDRC
K144-a ANCyEEVkDRCTLAE
K144-u ANCyEEVkDRCTLAE
T148 EEVkDRCTLAEKLGG
S156-p LAEKLGGsAVISLEG
K164-u AVISLEGkPL_____
3311 : Phospho-Cofilin (Ser3) Antibody
3313 : Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
  rat

 
S3-p _____MAsGVAVSDG
S8 MAsGVAVSDGVIKVF
K13 AVSDGVIKVFNDMKV
K13 AVSDGVIKVFNDMKV
K19 IKVFNDMKVRKSSTP
K19 IKVFNDMKVRKSSTP
K22 FNDMKVRKSSTPEEV
S23 NDMKVRKSSTPEEVK
S24 DMKVRKSSTPEEVKK
T25 MKVRKSSTPEEVKKR
K30 SSTPEEVKKRKKAVL
K33 PEEVKKRKKAVLFCL
S41 KAVLFCLSEDKKNII
K44 LFCLSEDKKNIILEE
K44 LFCLSEDKKNIILEE
K45 FCLSEDKKNIILEEG
K45 FCLSEDKKNIILEEG
K53-u NIILEEGkEILVGDV
Y68-p GQTVDDPyTTFVkML
T70 TVDDPyTTFVkMLPD
K73-u DPyTTFVkMLPDKDC
K73 DPyTTFVKMLPDKDC
K78 FVkMLPDKDCRYALY
K78 FVkMLPDKDCRYALY
Y82 LPDKDCRYALYDATy
Y85 KDCRYALYDATyETK
T88 RYALYDATyETKESK
Y89-p YALYDATyETKESKK
T91 LYDATyETKESKKED
K92 YDATyETKESKKEDL
K95 TyETKESKKEDLVFI
K96 yETKESKKEDLVFIF
K112-u APESAPLkSkMIYAS
K114-u ESAPLkSkMIYASSK
Y117 PLkSkMIYASSKDAI
K121 kMIYASSKDAIKKKL
K121 kMIYASSKDAIKKKL
K126 SSKDAIKKKLTGIKH
K127 SKDAIKKKLTGIKHE
K132 KKKLTGIKHELQANC
K132 KKKLTGIKHELQANC
Y140-p HELQANCyEEVKDRC
K144 ANCyEEVKDRCTLAE
K144 ANCyEEVKDRCTLAE
T148 EEVKDRCTLAEKLGG
S156-p LAEKLGGsAVISLEG
K164 AVISLEGKPL_____
3311 : Phospho-Cofilin (Ser3) Antibody
3313 : Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
  pig

 
S3-p _____MAsGVAVSDG
S8 MAsGVAVSDGVIKVF
K13 AVSDGVIKVFNDMKV
K13 AVSDGVIKVFNDMKV
K19 IKVFNDMKVRKSSTP
K19 IKVFNDMKVRKSSTP
K22 FNDMKVRKSSTPEEV
S23 NDMKVRKSSTPEEVK
S24 DMKVRKSSTPEEVKK
T25 MKVRKSSTPEEVKKR
K30 SSTPEEVKKRKKAVL
K33 PEEVKKRKKAVLFCL
S41 KAVLFCLSEDKKNII
K44 LFCLSEDKKNIILEE
K44 LFCLSEDKKNIILEE
K45 FCLSEDKKNIILEEG
K45 FCLSEDKKNIILEEG
K53 NIILEEGKEILVGDV
Y68 GQTVDDPYATFVKML
T70 TVDDPYATFVKMLPD
K73 DPYATFVKMLPDKDC
K73 DPYATFVKMLPDKDC
K78 FVKMLPDKDCRYALY
K78 FVKMLPDKDCRYALY
Y82 LPDKDCRYALYDATY
Y85 KDCRYALYDATYETK
T88 RYALYDATYETKESK
Y89 YALYDATYETKESKK
T91 LYDATYETKESKKED
K92 YDATYETKESKKEDL
K95 TYETKESKKEDLVFI
K96 YETKESKKEDLVFIF
K112 APECAPLKSKMIYAS
K114 ECAPLKSKMIYASSK
Y117 PLKSKMIYASSKDAI
K121 KMIYASSKDAIKKKL
K121 KMIYASSKDAIKKKL
K126 SSKDAIKKKLTGIKH
K127 SKDAIKKKLTGIKHE
K132 KKKLTGIKHELQANC
K132 KKKLTGIKHELQANC
Y140 HELQANCYEEVKDRC
K144 ANCYEEVKDRCTLAE
K144 ANCYEEVKDRCTLAE
T148 EEVKDRCTLAEKLGG
S156 LAEKLGGSAVISLEG
K164 AVISLEGKPL_____
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