Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
ubiquitin (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ubiquitin a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. Only the 76 amino acids of monoubiquitin product are shown in this entry. At the protein level, it is not possible to determine which of the four genes a given ubiquitin chain was derived from. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin-like modifier
Reference #:  P62988 (UniProtKB)
Alt. Names/Synonyms: 40S ribosomal protein S27a; CEP80; Ribosomal protein S27a; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP80; UBIQ; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80; ubiquitin-CEP80
Gene Symbols: UBA52-RPS27A-UBB-UBC
Molecular weight: 8,565 Da
Basal Isoelectric point: 6.56  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Angiogenesis  |  Death Receptor Signaling  |  Mitochondrial Control of Apoptosis  |  NF-kB Signaling  |  Notch Signaling  |  Parkinson's Disease  |  Toll-Like Receptor Signaling  |  Ubiquitin/Proteasome  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ubiquitin

Protein Structure Not Found.


STRING  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  DISEASE  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
4 9 K6-ac __MQIFVktLTGktI
2 162 K6-ub __MQIFVktLTGktI
0 2 T7-p _MQIFVktLTGktIt
5 1 K11-ac FVktLTGktItLEVE
4 163 K11-ub FVktLTGktItLEVE
1 0 K11-sm FVktLTGktItLEVE
0 3 T12-p VktLTGktItLEVEP
0 2 T14-p tLTGktItLEVEPSD
0 2 S20 ItLEVEPSDTIENVk
3 0 K27-ac SDTIENVkAkIQDkE
2 148 K27-ub SDTIENVkAkIQDkE
4 0 K29-ac TIENVkAkIQDkEGI
1 143 K29-ub TIENVkAkIQDkEGI
4 1 K33-ac VkAkIQDkEGIPPDQ
0 142 K33-ub VkAkIQDkEGIPPDQ
7 2 K48-ac QRLIFAGkQLEDGRT
13 167 K48-ub QRLIFAGkQLEDGRT
0 14 S57-p LEDGRTLsDyNIQkE
0 10 Y59-p DGRTLsDyNIQkEsT
6 1 K63-ac LsDyNIQkEsTLHLV
15 163 K63-ub LsDyNIQkEsTLHLV
0 1 K63-sm LsDyNIQkEsTLHLV
3 1 S65-p DyNIQkEsTLHLVLR
12805 : K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (HRP Conjugate)
12930 : K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate)
  mouse

 
K6-ac __MQIFVkTLTGkTI
K6-ub __MQIFVkTLTGkTI
T7 _MQIFVkTLTGkTIT
K11 FVkTLTGKTITLEVE
K11-ub FVkTLTGkTITLEVE
K11 FVkTLTGKTITLEVE
T12 VkTLTGkTITLEVEP
T14 TLTGkTITLEVEPsD
S20-p ITLEVEPsDTIENVk
K27 sDTIENVKAkIQDkE
K27-ub sDTIENVkAkIQDkE
K29 TIENVkAKIQDkEGI
K29-ub TIENVkAkIQDkEGI
K33-ac VkAkIQDkEGIPPDQ
K33-ub VkAkIQDkEGIPPDQ
K48-ac QRLIFAGkQLEDGRT
K48-ub QRLIFAGkQLEDGRT
S57-p LEDGRTLsDYNIQkE
Y59 DGRTLsDYNIQkEST
K63-ac LsDYNIQkESTLHLV
K63-ub LsDYNIQkESTLHLV
K63 LsDYNIQKESTLHLV
S65 DYNIQkESTLHLVLR
12805 : K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (HRP Conjugate)
12930 : K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate)
  rat

 
K6 __MQIFVKTLTGkTI
K6 __MQIFVKTLTGkTI
T7 _MQIFVKTLTGkTIT
K11-ac FVKTLTGkTITLEVE
K11 FVKTLTGKTITLEVE
K11 FVKTLTGKTITLEVE
T12 VKTLTGkTITLEVEP
T14 TLTGkTITLEVEPsD
S20-p ITLEVEPsDTIENVk
K27-ac sDTIENVkAKIQDkE
K27 sDTIENVKAKIQDkE
K29 TIENVkAKIQDkEGI
K29 TIENVkAKIQDkEGI
K33-ac VkAKIQDkEGIPPDQ
K33 VkAKIQDKEGIPPDQ
K48-ac QRLIFAGkQLEDGRT
K48 QRLIFAGKQLEDGRT
S57-p LEDGRTLsDYNIQkE
Y59 DGRTLsDYNIQkEST
K63-ac LsDYNIQkESTLHLV
K63 LsDYNIQKESTLHLV
K63 LsDYNIQKESTLHLV
S65 DYNIQkESTLHLVLR
12805 : K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (HRP Conjugate)
12930 : K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate)
  cow

 
K6 __MQIFVKTLTGKTI
K6-ub __MQIFVkTLTGKTI
T7 _MQIFVkTLTGKTIT
K11 FVkTLTGKTITLEVE
K11 FVkTLTGKTITLEVE
K11 FVkTLTGKTITLEVE
T12 VkTLTGKTITLEVEP
T14 TLTGKTITLEVEPSD
S20 ITLEVEPSDTIENVK
K27 SDTIENVKAKIQDKE
K27 SDTIENVKAKIQDKE
K29 TIENVKAKIQDKEGI
K29 TIENVKAKIQDKEGI
K33 VKAKIQDKEGIPPDQ
K33 VKAKIQDKEGIPPDQ
K48 QRLIFAGKQLEDGRT
K48 QRLIFAGKQLEDGRT
S57 LEDGRTLSDYNIQkE
Y59 DGRTLSDYNIQkEST
K63 LSDYNIQKESTLHLV
K63-ub LSDYNIQkESTLHLV
K63 LSDYNIQKESTLHLV
S65 DYNIQkESTLHLVLR
12805 : K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (HRP Conjugate)
12930 : K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (HRP Conjugate)
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.