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Protein Page:
NQO1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NQO1 a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Oxidoreductase; EC 1.6.5.2
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: cell soma; cytoplasm; cytosol
Molecular Function: protein binding; NAD(P)H dehydrogenase (quinone) activity; cytochrome-b5 reductase activity; superoxide dismutase activity
Biological Process: response to ethanol; removal of superoxide radicals; positive regulation of neuron apoptosis; response to toxin; xenobiotic metabolic process; negative regulation of catalytic activity; nitric oxide biosynthetic process; regulation of amino acid metabolic process; response to nutrient; response to estradiol stimulus; aging; synaptic transmission, cholinergic
Reference #:  P15559 (UniProtKB)
Alt. Names/Synonyms: Azoreductase; DHQU; DIA4; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); diaphorase-4; dioxin-inducible 1; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR1; NMORI; NQO1; Phylloquinone reductase; QR1; Quinone reductase 1
Gene Symbols: NQO1
Molecular weight: 30,868 Da
Basal Isoelectric point: 8.91  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NQO1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 17 Y20-p SERTSFNyAMkEAAA
0 1 K23-ub TSFNyAMkEAAAAAL
0 1 K31-ac EAAAAALkKkGWEVV
0 3 K31-ub EAAAAALkKkGWEVV
0 1 K31-sc EAAAAALkKkGWEVV
0 2 K33-ub AAAALkKkGWEVVEs
0 1 S40-p kGWEVVEsDLyAMNF
0 1 Y43-p EVVEsDLyAMNFNPI
0 1 K54-ub FNPIISRkDITGkLk
0 1 K59-ac SRkDITGkLkDPANF
0 2 K59-ub SRkDITGkLkDPANF
0 3 K61-ub kDITGkLkDPANFQy
0 2 Y68-p kDPANFQyPAESVLA
0 1 Y76-p PAESVLAykEGHLsP
0 5 K77-ac AESVLAykEGHLsPD
0 2 K77-ub AESVLAykEGHLsPD
0 5 S82-p AykEGHLsPDIVAEQ
0 3 K90-ub PDIVAEQkkLEAADL
0 1 K90-sc PDIVAEQkkLEAADL
0 2 K91-ub DIVAEQkkLEAADLV
0 1 Y127-p VFIGEFAyTyAAMyD
0 1 Y129-p IGEFAyTyAAMyDKG
0 1 Y133-p AyTyAAMyDKGPFRS
0 1 K141 DKGPFRSKKAVLSIT
0 11 K209-ac IQILEGWkkRLENIW
0 3 K209-ub IQILEGWkkRLENIW
0 1 K209-sc IQILEGWkkRLENIW
0 1 K210-ac QILEGWkkRLENIWD
0 3 K210-ub QILEGWkkRLENIWD
0 1 K241-ub QAGFLMKkEVQDEEk
0 1 K248-ub kEVQDEEkNKkFGLS
0 1 K251-ub QDEEkNKkFGLSVGH
0 2 K262-ac SVGHHLGkSIPTDNQ
0 1 K262-ub SVGHHLGkSIPTDNQ
0 3 K271-ub IPTDNQIkARK____
  NQO1 iso3  
Y20 SERTSFNYAMKEAAA
K23 TSFNYAMKEAAAAAL
K31 EAAAAALKKKGWEVV
K31 EAAAAALKKKGWEVV
K31 EAAAAALKKKGWEVV
K33 AAAALKKKGWEVVES
S40 KGWEVVESDLYAMNF
Y43 EVVESDLYAMNFNPI
K54 FNPIISRKDITGKLK
K59 SRKDITGKLKDPANF
K59 SRKDITGKLKDPANF
K61 KDITGKLKDPANFQY
Y68 KDPANFQYPAESVLA
Y76 PAESVLAYKEGHLSP
K77 AESVLAYKEGHLSPD
K77 AESVLAYKEGHLSPD
S82 AYKEGHLSPDIVAEQ
K90 PDIVAEQKkLEAADL
K90 PDIVAEQKkLEAADL
K91-ub DIVAEQKkLEAADLV
- gap
- gap
- gap
K103-ub DLVIFQSkKAVLSIT
K171 IQILEGWKKRLENIW
K171 IQILEGWKKRLENIW
K171 IQILEGWKKRLENIW
K172 QILEGWKKRLENIWD
K172 QILEGWKKRLENIWD
K203 QAGFLMKKEVQDEEK
K210 KEVQDEEKNKKFGLS
K213 QDEEKNKKFGLSVGH
K224 SVGHHLGKSIPTDNQ
K224 SVGHHLGKSIPTDNQ
K233 IPTDNQKKKKKKKKK
  mouse

 
Y20 SEKTSFNYAMKEAAV
K23 TSFNYAMKEAAVEAL
K31 EAAVEALKKRGWEVL
K31 EAAVEALKKRGWEVL
K31 EAAVEALKKRGWEVL
R33 AVEALKKRGWEVLES
S40 RGWEVLESDLYAMNF
Y43 EVLESDLYAMNFNPI
N54 FNPIISRNDITGELK
E59 SRNDITGELKDSKNF
E59 SRNDITGELKDSKNF
K61 NDITGELKDSKNFQY
Y68 KDSKNFQYPSESSLA
Y76 PSESSLAYkEGRLSP
K77 SESSLAYKEGRLSPD
K77-ub SESSLAYkEGRLSPD
S82 AYkEGRLSPDIVAEH
K90 PDIVAEHKKLEAADL
K90 PDIVAEHKKLEAADL
K91 DIVAEHKKLEAADLV
Y127 VLVAGFAYTYAAMYD
Y129 VAGFAYTYAAMYDNG
Y133 AYTYAAMYDNGPFQN
K141 DNGPFQNKKTLLSIT
K209 MQILEGWKKRLETVW
K209 MQILEGWKKRLETVW
K209 MQILEGWKKRLETVW
K210 QILEGWKKRLETVWE
K210 QILEGWKKRLETVWE
K241 QAGFLMKKEVQEEQK
K248 KEVQEEQKKNKFGLS
K251 QEEQKKNKFGLSVGH
K262 SVGHHLGKSIPADNQ
K262 SVGHHLGKSIPADNQ
K271 IPADNQIKARK____
  rat

 
Y20 AERTSFNYAMKEAAV
K23 TSFNYAMKEAAVEAL
K31 EAAVEALKKKGWEVV
K31 EAAVEALKKKGWEVV
K31 EAAVEALKKKGWEVV
K33 AVEALKKKGWEVVES
S40 KGWEVVESDLYAMNF
Y43 EVVESDLYAMNFNPL
N54 FNPLISRNDITGEPK
E59 SRNDITGEPKDSENF
E59 SRNDITGEPKDSENF
K61 NDITGEPKDSENFQY
Y68 KDSENFQYPVESSLA
Y76 PVESSLAYkEGRLsP
K77-ac VESSLAYkEGRLsPD
K77 VESSLAYKEGRLsPD
S82-p AYkEGRLsPDIVAEQ
K90 PDIVAEQKKLEAADL
K90 PDIVAEQKKLEAADL
K91 DIVAEQKKLEAADLV
Y127 VLVAGFAYTYATMYD
Y129 VAGFAYTYATMYDKG
Y133 AYTYATMYDKGPFQN
K141 DKGPFQNKKTLLSIT
K209 VQVLEGWKKRLETVW
K209 VQVLEGWKKRLETVW
K209 VQVLEGWKKRLETVW
K210 QVLEGWKKRLETVWE
K210 QVLEGWKKRLETVWE
K241 QAGFLLKKEVQEEQK
K248 KEVQEEQKKNKFGLS
K251 QEEQKKNKFGLSVGH
K262 SVGHHLGKSIPADNQ
K262 SVGHHLGKSIPADNQ
K271 IPADNQIKARK____
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