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Protein Page:
ARHGEF1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ARHGEF1 Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1. Interacts with CTNNAL1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; GEFs, Rac/Rho; GEFs
Cellular Component: cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; GTPase activator activity
Biological Process: cell proliferation; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; Rho protein signal transduction; positive regulation of Rho GTPase activity
Reference #:  Q92888 (UniProtKB)
Alt. Names/Synonyms: 115 kDa guanine nucleotide exchange factor; 115-kD protein; ARHG1; ARHGEF1; GEF1; LBCL2; LSC; Lsc homolog; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5
Gene Symbols: ARHGEF1
Molecular weight: 102,435 Da
Basal Isoelectric point: 5.47  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Microtubule Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ARHGEF1

Protein Structure Not Found.


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Sites Implicated In
activity, induced: Y738‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 - gap
0 2 S10-p DFARGAAsPGPSRPG
0 1 K234-ac RHLGVRTkSGDKKsG
0 6 S240-p TkSGDKKsGRNFFRK
0 2 K263-ub DEPAKTKkGLSsILD
0 1 S267-p KTKkGLSsILDAARW
0 1 D292-ca RHLKAEVdAEKPGAT
0 1 S325-p GQDTPGVsLHPLSLd
0 1 D332-ca sLHPLSLdSPDREPG
0 1 S350-p PLELGDSsPQGPMSL
0 1 - gap
0 1 - gap
0 1 - gap
0 7 - gap
0 1 - gap
0 1 - gap
0 2 - gap
0 7 - gap
0 2 - gap
0 8 S374-p DEGAETEsPEPGDEG
0 1 S409-p VPPDTLHsLPKSQVK
1 0 Y487-p KRRQESGyLIEEIGD
0 11 K510-ub AEGSWFQkISSRFCS
0 1 K567-ub TEMQRLTkYPLLLQS
0 1 S630-p DLSHLRQssDPMLSE
0 2 S631-p LSHLRQssDPMLSEF
0 1 K639-ub DPMLSEFkNLDITKK
0 5 T695-p KSHSRTLtPTPDGkT
0 1 T697 HSRTLtPTPDGkTML
0 1 K701-ub LtPTPDGkTMLRPVL
1 0 Y738-p WDQEAQIyELVAQTV
0 1 S776-p SRPKPRPsPSSTREP
0 3 S786-p STREPLLsSsENGNG
0 2 S788-p REPLLsSsENGNGGR
0 1 S798-p GNGGRETsPADARTE
0 1 R816 SDLLPFCRPGPEGQL
0 9 S863-p VPGGGPLsPARTQEI
0 5 - gap
0 2 T912-p SVPQPGCt_______
  ARHGEF1 iso3  
S9-p ASLSTWSsPAEPREM
S25 DFARGAASPGPSRPG
K249 RHLGVRTKSGDKKSG
S255 TKSGDKKSGRNFFRK
K278 DEPAKTKKGLSSILD
S282 KTKKGLSSILDAARW
D307 RHLKAEVDAEKPGAT
S340 GQDTPGVSLHPLSLD
D347 SLHPLSLDSPDREPG
S365 PLELGDSSPQGPMSL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S389 DEGAETESPEPGDEG
S424 VPPDTLHSLPKSQVK
Y502 KRRQESGYLIEEIGD
K525 AEGSWFQKISSRFCS
K582 TEMQRLTKYPLLLQS
S645 DLSHLRQSSDPMLSE
S646 LSHLRQSSDPMLSEF
K654 DPMLSEFKNLDITKK
T710 KSHSRTLTPTPDGKT
T712 HSRTLTPTPDGKTML
K716 LTPTPDGKTMLRPVL
Y753 WDQEAQIYELVAQTV
S791 SRPKPRPSPSSTREP
S801 STREPLLSSSENGNG
S803 REPLLSSSENGNGGR
S813 GNGGRETSPADARTE
R831 SDLLPFCRPGPEGQL
S878 VPGGGPLSPARTQEI
- gap
T927 SVPQPGCT_______
  ARHGEF1 iso4  
S54 GHLRADTSPAEPREM
S70 DFARGAASPGPSRPG
K294 RHLGVRTKSGDKKSG
S300 TKSGDKKSGRNFFRK
K323 DEPAKTKKGLSSILD
S327 KTKKGLSSILDAARW
D352 RHLKAEVDAEKPGAT
S385 GQDTPGVSLHPLSLD
D392 SLHPLSLDSPDREPG
S410 PLELGDSSPQGPMSL
T592-p PPCPLPPtPRLAPAS
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

► Hide Isoforms
 
- gap
- gap
K232 RHLGVRTKSGDKKSG
S238 TKSGDKKSGRNFFRK
K261 DEPPKTKKGLSSILD
S265 KTKKGLSSILDPARW
D290 RHLKVEADAEKPGPA
S323 GQDNPGVSLHPLSTD
D330 SLHPLSTDSVDSREP
P349 PQEPGDTPPQGPTSL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S373 EDNGETESPEPGDDG
S408 VPPDTLLSLPKSQVK
Y486 KRRQESGYLIEEIGD
K509 AEGSWFQKISSRFCS
K566 TEMQRLTKYPLLLQS
S629 DLTHLRQSSDPMLSE
S630 LTHLRQSSDPMLSEF
K638 DPMLSEFKNLDITKK
T694-p KSHSRTLtPtPDGKT
T696-p HSRTLtPtPDGKTML
K700 LtPtPDGKTMLRPVL
Y737 WDQEAQIYELVAQTS
S775 SRLKPRPSPSSIREP
S785 SIREPLLSSsENGTG
S787-p REPLLSSsENGTGGA
A797 GTGGAEMAPADARTE
R815-m1 NDLLPFCrPGPEGQL
G862 VPGGRAPGPVHTQEI
S905-p SQLGGTLsPNLAAPE
S920 RSAQTGLS_______
  ARHGEF1 iso2  
- gap
- gap
K232 RHLGVRTKSGDKKSG
S238 TKSGDKKSGRNFFRK
K261 DEPPKTKKGLSSILD
S265 KTKKGLSSILDPARW
D290 RHLKVEADEKPGPAD
S322 GQDNPGVSLHPLSTD
D329 SLHPLSTDSVDSREP
P348 PQEPGDTPPQGPTSL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S372 EDNGETESPEPGDDG
S407 VPPDTLLSLPKSQVK
Y485 KRRQESGYLIEEIGD
K508 AEGSWFQKISSRFCS
K565 TEMQRLTKYPLLLQS
S628 DLTHLRQSSDPMLSE
S629 LTHLRQSSDPMLSEF
K637 DPMLSEFKNLDITKK
T693 KSHSRTLTPTPDGKT
T695 HSRTLTPTPDGKTML
K699 LTPTPDGKTMLRPVL
Y736 WDQEAQIYELVAQTS
S774 SRLKPRPSPSSIREP
S784 SIREPLLSSSENGTG
S786 REPLLSSSENGTGGA
A796 GTGGAEMAPADARTE
R814 NDLLPFCRPGPEGQL
G861 VPGGRAPGPVHTQEI
S904 SQLGGTLSPNLAAPE
S919 RSAQTGLS_______
  ARHGEF1 iso5  
- gap
- gap
K232 RHLGVRTKSGDKKSG
S238 TKSGDKKSGRNFFRK
K261 DEPPKTKKGLSSILD
S265 KTKKGLSSILDPARW
D290 RHLKVEADEKPGPAD
S322 GQDNPGVSLHPLSTD
D329 SLHPLSTDSVDSREP
P348 PQEPGDTPPQGPTSL
- gap
S384-p LSGRLGRsEsLRVsD
S386-p GRLGRsEsLRVsDRR
S390-p RsEsLRVsDRRRPsR
S396-p VsDRRRPsRGsLGAK
S399-p RRRPsRGsLGAKGRG
S410-p KGRGGGRsRsDVDMD
S412-p RGGGRsRsDVDMDPG
S420-p DVDMDPGsATAVLGP
T432-p LGPTRRAtPEPGDDG
S467 VPPDTLLSLPKSQVK
Y545 KRRQESGYLIEEIGD
K568 AEGSWFQKISSRFCS
K625 TEMQRLTKYPLLLQS
S688 DLTHLRQSSDPMLSE
S689 LTHLRQSSDPMLSEF
K697 DPMLSEFKNLDITKK
T753 KSHSRTLTPTPDGKT
T755 HSRTLTPTPDGKTML
K759 LTPTPDGKTMLRPVL
Y796 WDQEAQIYELVAQTS
S834 SRLKPRPSPSSIREP
S844 SIREPLLSSSENGTG
S846 REPLLSSSENGTGGA
A856 GTGGAEMAPADARTE
R874 NDLLPFCRPGPEGQL
G921 VPGGRAPGPVHTQEI
S964-p SQLGGTLsPNLAAPE
S979 RSAQTGLS_______
  rat

 
- gap
- gap
K232 RHLGVRTKSGDKKSG
S238 TKSGDKKSGRNFFRK
K261 DEPPKTKKGLSSILD
S265 KTKKGLSSILDPARW
D290 RHLKVEVDEKPGPAD
S322 GQDNPGVSLHPLSVD
D329 SLHPLSVDSLDSREP
P348 PQEPGDTPPQGPTSL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S372 EDNGETESPEPGDDG
G407 VPSDTLLGLPKNQVK
Y485-p KRRQESGyLIEEIGD
K508 AEGSWFQKISSRFCS
K565 TEMQRLTKYPLLLQS
S628 DLTHLRQSNDPMLSE
N629 LTHLRQSNDPMLSEF
K637 DPMLSEFKNLDITKK
T693 SHTSRTLTPTPDGKT
T695 TSRTLTPTPDGKTML
K699 LTPTPDGKTMLRPVL
Y736-p WDQEAQIyELVAQTS
S774 SRPKPRPSPSSTREP
S784 STREPLLSSSENGTG
S786 REPLLSSSENGTGGT
A796 GTGGTEAAPADARTE
R814 NDLLPFCRPGPEGQL
G861 VPGGGAPGPTHTQEV
S904 SQLGEILSPNLAAPE
S919 RSAQTGLS_______
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