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Protein Page:
CFLAR (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CFLAR Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Apoptosis
Cellular Component: cytoplasm; CD95 death-inducing signaling complex; cytosol
Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; enzyme activator activity
Biological Process: positive regulation of catalytic activity; skeletal muscle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; skeletal myofibril assembly; skeletal muscle atrophy; viral reproduction; apoptosis; skeletal muscle regeneration; regulation of satellite cell proliferation; proteolysis; activation of NF-kappaB transcription factor; negative regulation of apoptosis
Reference #:  O15519 (UniProtKB)
Alt. Names/Synonyms: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD-like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8 and FADD-like apoptosis regulator subunit p43; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; Casper; Cellular FLICE-like inhibitory protein; CFLAR; CLARP; FADD-like anti-apoptotic molecule; FADD-like antiapoptotic molecule 1; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; Usurpin; usurpin beta
Gene Symbols: CFLAR
Molecular weight: 55,344 Da
Basal Isoelectric point: 8.19  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  Death Receptor Signaling  |  Inhibition of Apoptosis
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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CFLAR

Protein Structure Not Found.


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Sites Implicated In
protein degradation: S273‑p
ubiquitination: S193‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 3 K49-ub DILRERGkLSVGDLA
0 1 R122-m1 LMKDYMGrGKISKEK
1 0 T166-p IHRIDLKtkIQKyKQ
1 0 K167-ub HRIDLKtkIQKyKQS
0 3 Y171-p LKtkIQKyKQSVQGA
0 1 K172 KtkIQKyKQSVQGAG
0 2 Y182-p VQGAGTSyRNVLQAA
0 1 K192-ub VLQAAIQksLKDPSN
1 1 S193-p LQAAIQksLKDPSNN
0 1 S227-p QQEPVKKsIQEsEAF
0 1 S231-p VKKsIQEsEAFLPQS
1 0 S273-p LLRDTFTsLGYEVQK
0 1 S314-p SFVCVLVsRGGSQSV
  CFLAR iso2  
K49 DILRERGKLSVGDLA
R122 LMKDYMGRGKISKEK
T166 IHRIDLKTKIQKYKQ
K167 HRIDLKTKIQKYKQS
Y171 LKTKIQKYKQSVQGA
K172 KTKIQKYKQSVQGAG
Y182 VQGAGTSYRNVLQAA
K192 VLQAAIQKsLKDPSN
S193-p LQAAIQKsLKDPSNN
- gap
- gap
- gap
- gap
  mouse

 
Q54 DSLSERGQLSFATLA
R127 LTRDYTGRGKIAKDK
T171 IHRIDLNTKIQKYtQ
K172 HRIDLNTKIQKYtQS
Y176 LNTKIQKYtQSSQGA
T177-p NTKIQKYtQSSQGAR
N186 SSQGARSNMNTLQAS
K196 TLQASLPKLSIKPSV
S198 QASLPKLSIKPSVLY
S232 QRTLVKTSIQESGAF
S236 VKTSIQESGAFLPPH
S278 YLQETFTSLGYHIQL
S319 SFACVLVSLGGSQSM
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