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Protein Page:
K8 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
K8 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein
Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; costamere; nuclear matrix; keratin filament; cytoplasm; intermediate filament; Z disc; nucleus; sarcolemma
Molecular Function: protein binding; protein complex binding; structural molecule activity
Biological Process: viral reproduction; tumor necrosis factor-mediated signaling pathway; response to other organism; sarcomere organization; response to hydrostatic pressure
Reference #:  P05787 (UniProtKB)
Alt. Names/Synonyms: CARD2; CK-8; CK8; CYK8; cytokeratin 8; Cytokeratin-8; K2C8; K8; keratin 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; KRT8; Type-II keratin Kb8
Gene Symbols: KRT8
Molecular weight: 53,704 Da
Basal Isoelectric point: 5.52  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

K8

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
cell motility, altered: S74‑p, S432‑p
cytoskeletal reorganization: S74‑p, S432‑p
intracellular localization: S74‑p, S432‑p
molecular association, regulation: S74‑p
protein conformation: S291‑p
protein stabilization: S74‑p
ubiquitination: S74‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S2-p ______MsIRVtQks
0 1 T6-p __MsIRVtQksYkVs
0 2 K8-u MsIRVtQksYkVsTs
2 23 S9-p sIRVtQksYkVsTsG
2 1 K11-a RVtQksYkVsTsGPr
0 6 K11-u RVtQksYkVsTsGPr
1 4 S13-p tQksYkVsTsGPrAF
0 1 S13-gl tQksYkVsTsGPrAF
0 1 T14 QksYkVsTsGPrAFs
0 1 S15-gl ksYkVsTsGPrAFss
0 1 S15 ksYkVsTSGPrAFss
0 1 R18-m1 kVsTsGPrAFssrsy
0 38 S21-p TsGPrAFssrsytsG
0 22 S22-p sGPrAFssrsytsGP
0 5 R23-m1 GPrAFssrsytsGPG
5 101 S24-p PrAFssrsytsGPGs
0 80 Y25-p rAFssrsytsGPGsr
0 21 T26-p AFssrsytsGPGsrI
0 30 S27-p FssrsytsGPGsrIs
0 16 S31-p sytsGPGsrIssssF
0 7 R32-m1 ytsGPGsrIssssFs
1 43 S34-p sGPGsrIssssFsRV
0 99 S35-p GPGsrIssssFsRVG
0 11 S36-p PGsrIssssFsRVGs
1 21 S37-p GsrIssssFsRVGss
0 19 S39-p rIssssFsRVGssNF
1 70 S43-p ssFsRVGssNFrGGL
0 17 S44-p sFsRVGssNFrGGLG
0 1 - gap
0 4 - gap
1 3 - gap
0 9 R47-m1 RVGssNFrGGLGGGY
0 3 R47-m2 RVGssNFrGGLGGGY
1 1 G49 GssNFrGGLGGGYGG
0 1 T64-p ASGMGGItAVTVNQS
25 10 S74-p TVNQSLLsPLVLEVD
0 2 K92 QAVRTQEKEQIkTLN
0 2 K92-u QAVRTQEkEQIkTLN
0 6 K96-u TQEkEQIkTLNNkFA
2 30 K101-a QIkTLNNkFAsFIDk
0 9 K101-u QIkTLNNkFAsFIDk
0 17 S104-p TLNNkFAsFIDkVRF
0 6 K108-a kFAsFIDkVRFLEQQ
0 10 K108-u kFAsFIDkVRFLEQQ
0 2 K117-a RFLEQQNkMLETkWs
0 9 K117-u RFLEQQNkMLETkWs
0 5 K122-a QNkMLETkWsLLQQQ
0 7 K122-u QNkMLETkWsLLQQQ
0 1 S124-p kMLETkWsLLQQQkT
0 10 K130-u WsLLQQQkTARSNMD
0 2 S142-p NMDNMFEsyINNLRR
0 7 Y143-p MDNMFEsyINNLRRQ
0 2 K158-u LETLGQEkLkLEAEL
0 1 K160-u TLGQEkLkLEAELGN
0 1 K176-u QGLVEDFkNKYEDEI
0 2 K178 LVEDFkNKYEDEINK
0 1 T187-p EDEINKRtEMENEFV
0 2 K197-u ENEFVLIkkDVDEAy
0 2 K198-u NEFVLIkkDVDEAyM
1 104 Y204-p kkDVDEAyMNkVELE
1 2 K207-a VDEAyMNkVELESRL
0 3 K207-u VDEAyMNkVELESRL
0 1 Y228-p INFLRQLyEEEIREL
0 14 S253-p LSMDNSRsLDMDsII
0 7 S258-p SRsLDMDsIIAEVkA
0 6 K264-u DsIIAEVkAQyEDIA
1 433 Y267-p IAEVkAQyEDIANRs
0 7 S274-p yEDIANRsRAEAEsM
0 7 S280-p RsRAEAEsMyQIkyE
0 101 Y282-p RAEAEsMyQIkyEEL
0 3 K285-a AEsMyQIkyEELQsL
1 0 K285-s AEsMyQIkyEELQsL
0 5 K285-u AEsMyQIkyEELQsL
0 38 Y286-p EsMyQIkyEELQsLA
1 3 S291-p IkyEELQsLAGkHGD
0 2 K295-a ELQsLAGkHGDDLRR
0 3 K295-u ELQsLAGkHGDDLRR
0 2 T303-p HGDDLRRtktEIsEM
0 8 K304-u GDDLRRtktEIsEMN
0 31 T305-p DDLRRtktEIsEMNR
0 1 S308-p RRtktEIsEMNRNIs
0 3 S315-p sEMNRNIsRLQAEIE
0 2 K325-a QAEIEGLkGQRAsLE
0 22 K325-u QAEIEGLkGQRAsLE
0 6 S330-p GLkGQRAsLEAAIAD
0 7 K347-u QRGELAIkDANAkLS
0 5 K352-u AIkDANAkLSELEAA
1 0 K364-s EAALQRAkQDMARQL
1 1 K393-a IEIATYRkLLEGEEs
0 59 K393-u IEIATYRkLLEGEEs
0 2 S400-p kLLEGEEsRLEsGMQ
0 1 S404-p GEEsRLEsGMQNMsI
0 3 S410-p EsGMQNMsIHTKTts
0 1 T416-p MsIHTKTtsGyAGGL
1 1 S417-p sIHTKTtsGyAGGLs
0 7 Y419-p HTKTtsGyAGGLsSA
0 1 S424-p sGyAGGLsSAyGGLt
1 0 A426 yAGGLsSAyGGLtsP
0 17 Y427-p AGGLsSAyGGLtsPG
1 1 T431-p sSAyGGLtsPGLsys
20 21 S432-p SAyGGLtsPGLsysL
0 6 S436-p GLtsPGLsysLGssF
0 50 Y437-p LtsPGLsysLGssFG
0 1 S438-p tsPGLsysLGssFGs
0 2 S441-p GLsysLGssFGsGAG
0 7 S442-p LsysLGssFGsGAGs
0 7 S445-p sLGssFGsGAGsSsF
0 1 S449-p sFGsGAGsSsFSRts
0 3 S451-p GsGAGsSsFSRtsss
0 7 T455-p GsSsFSRtsssRAVV
0 9 S456-p sSsFSRtsssRAVVV
0 16 S457-p SsFSRtsssRAVVVk
0 2 S458-p sFSRtsssRAVVVkk
0 3 K464-a ssRAVVVkkIETRDG
0 3 K465-a sRAVVVkkIETRDGk
2 3 K472-a kIETRDGkLVsEssD
0 11 K472-u kIETRDGkLVsEssD
0 14 S475-p TRDGkLVsEssDVLP
0 1 S475-gl TRDGkLVsEssDVLP
0 2 S477-p DGkLVsEssDVLPk_
0 7 S478-p GkLVsEssDVLPk__
0 5 P482 sEssDVLPk______
3 38 K483-a EssDVLPk_______
0 3 K483 EssDVLPK_______
  mouse

 
S2 ______MSIRVTQKs
T6 __MSIRVTQKsYkMs
K8 MSIRVTQKsYkMsts
S9-p SIRVTQKsYkMstsG
K11 RVTQKsYKMstsGPr
K11-u RVTQKsYkMstsGPr
S13-p TQKsYkMstsGPrAF
S13 TQKsYkMStsGPrAF
T14-p QKsYkMstsGPrAFs
S15 KsYkMstSGPrAFss
S15-p KsYkMstsGPrAFss
R18-m1 kMstsGPrAFssRsF
S21-p tsGPrAFssRsFtsG
S22-p sGPrAFssRsFtsGP
R23 GPrAFssRsFtsGPG
S24-p PrAFssRsFtsGPGA
F25 rAFssRsFtsGPGAR
T26-p AFssRsFtsGPGARI
S27-p FssRsFtsGPGARIs
A31 sFtsGPGARIssssF
R32 FtsGPGARIssssFs
S34-p sGPGARIssssFsRV
S35-p GPGARIssssFsRVG
S36-p PGARIssssFsRVGs
S37-p GARIssssFsRVGss
S39-p RIssssFsRVGssSs
S43-p ssFsRVGssSssFRG
S44-p sFsRVGssSssFRGS
S45 FsRVGssSssFRGSM
S46-p sRVGssSssFRGSMG
S47-p RVGssSssFRGSMGT
R49 GssSssFRGSMGTGV
R49 GssSssFRGSMGTGV
S51 sSssFRGSMGTGVGL
T70 GAGVGGITAVTVNQS
S80-p TVNQSLLsPLKLEVD
K98-a QAVRTQEkEQIKSLN
K98 QAVRTQEKEQIKSLN
K102 TQEkEQIKSLNNkFA
K107-a QIKSLNNkFAsFIDk
K107-u QIKSLNNkFAsFIDk
S110-p SLNNkFAsFIDkVRF
K114 kFAsFIDKVRFLEQQ
K114-u kFAsFIDkVRFLEQQ
K123 RFLEQQNKMLETkWS
K123-u RFLEQQNkMLETkWS
K128-a QNkMLETkWSLLQQQ
K128-u QNkMLETkWSLLQQQ
S130 kMLETkWSLLQQQkT
K136-u WSLLQQQkTSRSNMD
S148 NMDNMFESYINNLRR
Y149 MDNMFESYINNLRRQ
K164-u LEALGQEkLKLEAEL
K166 ALGQEkLKLEAELGN
K182 QGLVEDFKNkYEDEI
K184-a LVEDFKNkYEDEINK
T193 EDEINKRTEMENEFV
K203-u ENEFVLIkkDVDEAy
K204-u NEFVLIkkDVDEAyM
Y210-p kkDVDEAyMNkVELE
K213 VDEAyMNKVELESRL
K213-u VDEAyMNkVELESRL
H234 INFLRQIHEEEIREL
S259-p LSMDNSRsLDMDGII
G264 SRsLDMDGIIAEVRA
R270 DGIIAEVRAQyEDIA
Y273-p IAEVRAQyEDIANRS
S280 yEDIANRSRAEAETM
T286 RSRAEAETMYQIKYE
Y288 RAEAETMYQIKYEEL
K291 AETMYQIKYEELQTL
K291 AETMYQIKYEELQTL
K291 AETMYQIKYEELQTL
Y292 ETMYQIKYEELQTLA
T297 IKYEELQTLAGkHGD
K301 ELQTLAGKHGDDLRR
K301-u ELQTLAGkHGDDLRR
T309 HGDDLRRTktEISEM
K310-u GDDLRRTktEISEMN
T311-p DDLRRTktEISEMNR
S314 RRTktEISEMNRNIN
N321 SEMNRNINRLQAEIE
K331-a QAEIEALkGQRAsLE
K331-u QAEIEALkGQRAsLE
S336-p ALkGQRAsLEAAIAD
K353-u QRGEMAIkDAQTKLA
K358 AIkDAQTKLAELEAA
K370 EAALQRAKQDMARQL
K399 IEITTYRKLLEGEES
K399-u IEITTYRkLLEGEES
S406 kLLEGEESRLESGMQ
S410 GEESRLESGMQNMsI
S416-p ESGMQNMsIHTKTTS
T422 MsIHTKTTSGYSGGL
S423 sIHTKTTSGYSGGLS
Y425 HTKTTSGYSGGLSSS
S430 SGYSGGLSSSYGGLT
S432 YSGGLSSSYGGLTsP
Y433 SGGLSSSYGGLTsPG
T437 SSSYGGLTsPGFSYG
S438-p SSYGGLTsPGFSYGM
S442 GLTsPGFSYGMSSFQ
Y443 LTsPGFSYGMSSFQP
S447 GFSYGMSSFQPGFGS
P450 YGMSSFQPGFGSAGG
G451 GMSSFQPGFGSAGGS
S454 SFQPGFGSAGGSNTF
S458 GFGSAGGSNTFSRTT
T460 GSAGGSNTFSRTTKA
T464 GSNTFSRTTKAVVVK
- gap
- gap
T465 SNTFSRTTKAVVVKK
K471 TTKAVVVKKIETRDG
K472 TKAVVVKKIETRDGk
K479-a KIETRDGkLVsESsD
K479-u KIETRDGkLVsESsD
S482-p TRDGkLVsESsDVVs
S482 TRDGkLVSESsDVVs
S484 DGkLVsESsDVVsk_
S485-p GkLVsESsDVVsk__
S489-p sESsDVVsk______
K490-a ESsDVVsk_______
K490-u ESsDVVsk_______
  rat

 
S2 ______MSVRVTQKs
T6 __MSVRVTQKsYKMs
K8 MSVRVTQKsYKMsTS
S9-p SVRVTQKsYKMsTSG
K11 RVTQKsYKMsTSGPR
K11 RVTQKsYKMsTSGPR
S13-p TQKsYKMsTSGPRAF
S13 TQKsYKMSTSGPRAF
T14 QKsYKMsTSGPRAFs
S15 KsYKMsTSGPRAFss
S15 KsYKMsTSGPRAFss
R18 KMsTSGPRAFssRsF
S21-p TSGPRAFssRsFtsG
S22-p SGPRAFssRsFtsGP
R23 GPRAFssRsFtsGPG
S24-p PRAFssRsFtsGPGA
F25 RAFssRsFtsGPGAR
T26-p AFssRsFtsGPGARI
S27-p FssRsFtsGPGARIs
A31 sFtsGPGARIsSSsF
R32 FtsGPGARIsSSsFS
S34-p sGPGARIsSSsFSRV
S35 GPGARIsSSsFSRVG
S36 PGARIsSSsFSRVGs
S37-p GARIsSSsFSRVGss
S39 RIsSSsFSRVGssss
S43-p SsFSRVGsssssFRG
S44-p sFSRVGsssssFRGs
S45-p FSRVGsssssFRGsL
S46-p SRVGsssssFRGsLG
S47-p RVGsssssFRGsLGG
R49 GsssssFRGsLGGFG
R49 GsssssFRGsLGGFG
S51-p ssssFRGsLGGFGGA
T64 GAGVGGITAVTVNQS
N74 TVNQSLLNPLKLEVD
K92 QAVRTQEKEQIKTLN
K92 QAVRTQEKEQIKTLN
K96 TQEKEQIKTLNNKFA
K101 QIKTLNNKFASFIDK
K101 QIKTLNNKFASFIDK
S104 TLNNKFASFIDKVRF
K108 KFASFIDKVRFLEQQ
K108 KFASFIDKVRFLEQQ
K117 RFLEQQNKMLETKWS
K117 RFLEQQNKMLETKWS
K122 QNKMLETKWSLLQQQ
K122 QNKMLETKWSLLQQQ
S124 KMLETKWSLLQQQKT
K130 WSLLQQQKTSRSNMD
S142 NMDNMFESYINNLRR
Y143 MDNMFESYINNLRRQ
K158 LEALGQEKLKLEVEL
K160 ALGQEKLKLEVELGN
K176 QGLVEDFKNKYEDEI
K178 LVEDFKNKYEDEINK
T187 EDEINKRTEMENEFV
K197 ENEFVLIKKDVDEAY
K198 NEFVLIKKDVDEAYM
Y204 KKDVDEAYMNKVELE
K207 VDEAYMNKVELESRL
K207 VDEAYMNKVELESRL
H228 INFLRQIHEEEIREL
S253-p LSMDNSRsLDMDsII
S258-p SRsLDMDsIIAEVRA
R264 DsIIAEVRAQYEEIA
Y267 IAEVRAQYEEIANRS
S274 YEEIANRSRAEAETM
T280 RSRAEAETMYQIKYE
Y282 RAEAETMYQIKYEEL
K285 AETMYQIKYEELQTL
K285 AETMYQIKYEELQTL
K285 AETMYQIKYEELQTL
Y286 ETMYQIKYEELQTLA
T291 IKYEELQTLAGKHGD
K295 ELQTLAGKHGDDLRR
K295 ELQTLAGKHGDDLRR
S303 HGDDLRRSKTEISEM
K304 GDDLRRSKTEISEMN
T305 DDLRRSKTEISEMNR
S308 RRSKTEISEMNRNIS
S315 SEMNRNISRLQAEID
K325 QAEIDALKGQRATLE
K325 QAEIDALKGQRATLE
T330 ALKGQRATLEAAIAD
K347 QRGELAVKDANAKLE
K352 AVKDANAKLEDLKNA
K364 KNALQKAKQDMARQL
K393 IEIATYRKLLEGEES
K393 IEIATYRKLLEGEES
S400 KLLEGEESRLESGMQ
S404 GEESRLESGMQNMSI
S410 ESGMQNMSIHTKTTs
T416 MSIHTKTTsGYAGGL
S417-p SIHTKTTsGYAGGLS
Y419 HTKTTsGYAGGLSSs
S424 sGYAGGLSSsYGGLT
S426-p YAGGLSSsYGGLTsP
Y427 AGGLSSsYGGLTsPG
T431 SSsYGGLTsPGFSYG
S432-p SsYGGLTsPGFSYGM
S436 GLTsPGFSYGMSSFQ
Y437 LTsPGFSYGMSSFQP
S441 GFSYGMSSFQPGFGS
P444 YGMSSFQPGFGSVGG
G445 GMSSFQPGFGSVGGS
S448 SFQPGFGSVGGSSTY
S452 GFGSVGGSSTYSRTK
T454 GSVGGSSTYSRTKAV
T458 GSSTYSRTKAVVVKK
- gap
- gap
- gap
K464 RTKAVVVKKIETRDG
K465 TKAVVVKKIETRDGK
K472 KIETRDGKLVsEssD
K472 KIETRDGKLVsEssD
S475-p TRDGKLVsEssDIMs
S475 TRDGKLVSEssDIMs
S477-p DGKLVsEssDIMsK_
S478-p GKLVsEssDIMsK__
S482-p sEssDIMsK______
K483 EssDIMsK_______
K483 EssDIMsK_______
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