The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: microtubule cytoskeleton; protein phosphatase type 2A complex; membrane; mitochondrion; cytosol; nucleus; chromosome, pericentric region
Molecular Function: protein binding; protein heterodimerization activity; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity; antigen binding
Biological Process: regulation of cell adhesion; second-messenger-mediated signaling; mitotic nuclear envelope reassembly; fibroblast growth factor receptor signaling pathway; apoptosis; RNA splicing; regulation of Wnt receptor signaling pathway; protein amino acid dephosphorylation; chromosome segregation; mRNA metabolic process; response to organic substance; regulation of transcription, DNA-dependent; RNA metabolic process; regulation of cell differentiation; regulation of growth; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; protein complex assembly; gene expression; negative regulation of cell growth; mitotic cell cycle; ceramide metabolic process; G2/M transition of mitotic cell cycle; regulation of DNA replication; inactivation of MAPK activity
SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.