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SMURF2 (human)

Overview SMURF2 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Note: This description may include information from UniProtKB. Protein type: Ligase; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol; nucleus; ubiquitin ligase complex; lipid raft Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity; SMAD binding Biological Process: BMP signaling pathway; ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; negative regulation of transcription, DNA-dependent Reference #:  Q9HAU4 (UniProtKB) Alt. Names/Synonyms: DKFZp686F0270; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; hSMURF2; MGC138150; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMUF2; SMURF2 Gene Symbols: SMURF2 Molecular weight: 86,196 Da Basal Isoelectric point: 8.18  Predict pI for various phosphorylation states CST Pathways:  TGF-ß Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below SMURF2 1ZVD - A=369-748 (human) 2DJY - A=297-333 (human) 2JQZ - A=10-140 (human) 2KXQ - A=250-333 (human) 2LTZ - B=203-217 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1ZVD 2DJY 2JQZ 2KXQ 2LTZ  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		T168-p	DGWEERRtAsGRIQY
0	1		S170-p	WEERRtAsGRIQYLN
0	2		S203-p	SSPGRPLsCFVDENT
0	1		K345	LNRQNQLKDQQQQQV
0	3		Y434-p	RGEEGLDyGGVAREW
0	1		Y499-p	MAVFHGHyIDGGFTL
0	1		Y509	GGFTLPFYKQLLGKS
0	1		K615	VIPQHLLKTFDEKEL

	mouse
T168	DGWEERRTASGRIQY
S170	WEERRTASGRIQYLN
S203	SSPGRPLSCFVDENT
K345-u	LNRQNQLkDQQQQQV
Y434	RGEEGLDYGGVAREW
Y499	MAVFHGHYIDGGFTL
Y509	GGFTLPFYKQLLGKS
K615-u	VIPQHLLkTFDEKEL

	rat
T168	DGWEERRTASGRIQY
S170	WEERRTASGRIQYLN
S203	SSPGRPLSCFVDENT
K345	LNRQNQLKDQQQQQV
Y434-p	RGEEGLDyGGVAREW
Y499	MAVFHGHYIDGGFTL
Y509-p	GGFTLPFyKQLLGKS
K615	VIPQHLLKTFDEKEL

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