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Protein Page:
SMURF2 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
SMURF2 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system; EC 6.3.2.19
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol; nucleus; ubiquitin ligase complex; lipid raft
Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity; SMAD binding; transforming growth factor beta receptor binding
Biological Process: ubiquitin-dependent protein catabolic process; BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; gene expression; negative regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent SMAD protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway
Reference #:  Q9HAU4 (UniProtKB)
Alt. Names/Synonyms: DKFZp686F0270; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; hSMURF2; MGC138150; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMUF2; SMURF2
Gene Symbols: SMURF2
Molecular weight: 86,196 Da
Basal Isoelectric point: 8.18  Predict pI for various phosphorylation states
CST Pathways:  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SMURF2

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

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 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S203-p SSPGRPLsCFVDENT
0 1 K345 LNRQNQLKDQQQQQV
0 3 Y434-p RGEEGLDyGGVAREW
0 1 K615 VIPQHLLKTFDEKEL
  mouse

 
S203 SSPGRPLSCFVDENT
K345-u LNRQNQLkDQQQQQV
Y434 RGEEGLDYGGVAREW
K615-u VIPQHLLkTFDEKEL
  rat

 
S203 SSPGRPLSCFVDENT
K345 LNRQNQLKDQQQQQV
Y434-p RGEEGLDyGGVAREW
K615 VIPQHLLKTFDEKEL
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