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Protein Page:
rubicon (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
rubicon Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity. Forms a complex with BECN1, PIK3C3, PIK3R4 and UVRAG, but not with ATG14. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Unknown function
Cellular Component: Golgi apparatus; lysosome; early endosome; late endosome
Molecular Function: protein binding
Biological Process: negative regulation of autophagy; negative regulation of endocytosis; autophagy; endocytosis
Reference #:  Q92622 (UniProtKB)
Alt. Names/Synonyms: hypothetical protein LOC9711; K0226; KIAA0226; Uncharacterized protein KIAA0226
Gene Symbols: KIAA0226
Molecular weight: 108,622 Da
Basal Isoelectric point: 5.86  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

rubicon

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S44-p EGLVSTNsPNVWSKY
0 1 S92-p VKDIRWLsPHSALHV
0 6 S197-p MFARKHEsPLLVTKS
0 1 S206 LLVTKSQSLTALPSS
0 1 S225 PNSYAQHSYFGSFSS
0 1 Y226 NSYAQHSYFGSFSSL
0 1 S229 AQHSYFGSFSSLHQS
0 1 S232 SYFGSFSSLHQSVPN
0 1 S246 NNGSERRStsFPLSG
0 1 T247-p NGSERRStsFPLSGP
0 4 S248-p GSERRStsFPLSGPP
0 5 S266-p QESRGHVsPAEDQtI
0 1 T272-p VsPAEDQtIQAPPVS
0 1 S378-p DQEGGGEsQLSSVLR
0 8 S387-p LSSVLRRssFSEGQT
0 55 S388-p SSVLRRssFSEGQTL
0 3 S390 VLRRssFSEGQTLTV
0 1 T394 ssFSEGQTLTVTSGA
0 1 K403-ub TVTSGAKkSHIRsHs
0 1 S408-p AKkSHIRsHsDTSIA
0 20 S410-p kSHIRsHsDTSIAsR
0 1 S416-p HsDTSIAsRGAPESC
0 1 T444-p GQSSEVStPSSLyME
0 7 Y449-p VStPSSLyMEYEGGR
0 2 Y457-p MEYEGGRyLCSGEGM
0 10 S469-p EGMFRRPsEGQsLIS
0 1 S473-p RRPsEGQsLISYLSE
0 3 S528-p EEVEEEDsDREIQEL
0 1 S562-p YQEAEHGsFRVTSSS
0 1 S570-p FRVTSSSsQFSSRDs
0 1 S573 TSSSsQFSSRDsAQL
0 1 S577-p sQFSSRDsAQLsDsG
0 2 S581-p SRDsAQLsDsGsADE
0 3 S583-p DsAQLsDsGsADEVD
0 2 S585-p AQLsDsGsADEVDEF
0 1 S609-p NTASSSKsFVsSQsF
0 1 S612-p SSSKsFVsSQsFSHC
0 1 S615-p KsFVsSQsFSHCFLH
0 6 S671-p IPDSLPIsPDDGQHA
0 2 T709-p FNVHPAPtRKIAVAK
0 2 T728-p CAGCGIRtDPDyIKR
0 1 Y732-p GIRtDPDyIKRLRYC
0 1 Y772-p KWDFSKYyVsNFsKD
0 1 S774-p DFSKYyVsNFsKDLL
0 1 S777-p KYyVsNFsKDLLIKI
0 1 S851-p TEDLHLYsLNDLtAT
0 1 T856-p LYsLNDLtATRKGEL
0 1 S952-p QSLESYLsDYEEEPA
  mouse

► Hide Isoforms
 
C45 EGLVSANCPNVWSKY
S93 VKDIRWLSPHSALHV
S198-p MFARKQEsPLLVTKS
S207-p LLVTKSQsLTALPGS
S226-p PASYAQHsyFGsSSs
Y227-p ASYAQHsyFGsSSsL
S230-p AQHsyFGsSSsLQSM
S233-p syFGsSSsLQSMPQS
S248-p SHSSERRsTSFSLSG
T249 HSSERRsTSFSLSGP
S250 SSERRsTSFSLSGPS
S268 QEDRECLSPAETQTT
T274 LSPAETQTTPAPLPS
S380 DQEEGRQSQAGSVLR
S389-p AGSVLRRssFsEGQt
S390-p GSVLRRssFsEGQtA
S392-p VLRRssFsEGQtAPV
T396-p ssFsEGQtAPVASGT
K405 PVASGTKKSHIRSHs
S410 TKKSHIRSHsDTNIA
S412-p KSHIRSHsDTNIASR
S418 HsDTNIASRGAAGGP
- gap
- gap
Y442 PIAEGGQYLCSGEGM
S454-p EGMFRRPsEGQSLIS
S458 RRPsEGQSLISYLSE
S513 EEVEEEDSDREIQEL
S547 YRETENGSFRVTSSS
S555 FRVTSSSSQFsSRDS
S558-p TSSSSQFsSRDSTQL
S562 SQFsSRDSTQLSEsG
S566 SRDSTQLSEsGSAED
S568-p DSTQLSEsGSAEDAD
S570 TQLSEsGSAEDADDL
S594 SAVSNGKSSFSQNLS
S597 SNGKSSFSQNLSHCF
N599 GKSSFSQNLSHCFLH
S655 IPDSLPISPDDGQHA
T693 FNVHPAPTRKIAVAK
T712 CAGCGIRTDPDYIKR
Y716 GIRTDPDYIKRLRYC
Y756 KWDFSKYYVSNFSKD
S758 DFSKYYVSNFSKDLL
S761 KYYVSNFSKDLLLKI
S835 TEDLHLYSLSDLTAT
T840 LYSLSDLTATKKGEL
S936 QSLESYLSDYEEEPT
  rubicon iso2  
C45 EGLVSANCPNVWSKY
S93 VKDIRWLSPHSALHV
S198 MFARKQESPLLVTKS
S207 LLVTKSQSLTALPGS
S226 PASYAQHSYFGSSSS
Y227 ASYAQHSYFGSSSSL
S230 AQHSYFGSSSSLQSM
S233 SYFGSSSSLQSMPQS
S248 SHSSERRSTSFSLSG
T249 HSSERRSTSFSLSGP
S250 SSERRSTSFSLSGPS
S268 QEDRECLSPAETQTT
T274 LSPAETQTTPAPLPS
S380 DQEEGRQSQAGSVLR
S389 AGSVLRRSSFSEGQT
S390 GSVLRRSSFSEGQTA
S392 VLRRSSFSEGQTAPV
T396 SSFSEGQTAPVASGT
K405 PVASGTKKSHIRSHS
S410 TKKSHIRSHSDTNIA
S412 KSHIRSHSDTNIASR
S418 HSDTNIASRGAAEGG
- gap
- gap
Y427 GAAEGGQYLCSGEGM
S439 EGMFRRPSEGQSLIS
S443 RRPSEGQSLISYLSE
S498 EEVEEEDSDREIQEL
S532 YRETENGSFRVTSSS
S540 FRVTSSSSQFSSRDS
S543 TSSSSQFSSRDSTQL
S547 SQFSSRDSTQLSESG
S551 SRDSTQLSESGSAED
S553 DSTQLSESGSAEDAD
S555 TQLSESGSAEDADDL
S579 SAVSNGKSSFSQNLS
S582 SNGKSSFSQNLSHCF
N584 GKSSFSQNLSHCFLH
S640 IPDSLPISPDDGQHA
T678 FNVHPAPTRKIAVAK
T697 CAGCGIRTDPDYIKR
Y701 GIRTDPDYIKRLRYC
Y741 KWDFSKYYVSNFSKD
S743 DFSKYYVSNFSKDLL
S746 KYYVSNFSKDLLLKI
S820 TEDLHLYSLSDLTAT
T825 LYSLSDLTATKKGEL
S921 QSLESYLSDYEEEPT
  rubicon iso3  
C45 EGLVSANCPNVWSKY
S93 VKDIRWLSPHSALHV
S198 MFARKQESPLLVTKS
S207 LLVTKSQSLTALPGS
S226 PASYAQHSYFGSSSS
Y227 ASYAQHSYFGSSSSL
S230 AQHSYFGSSSSLQSM
S233 SYFGSSSSLQSMPQS
S248 SHSSERRSTSFSLSG
T249 HSSERRSTSFSLSGP
S250 SSERRSTSFSLSGPS
S268 QEDRECLSPAETQTT
T274 LSPAETQTTPAPLPS
S366 DQEEGRQSQAGSVLR
S375 AGSVLRRSSFSEGQT
S376 GSVLRRSSFSEGQTA
S378 VLRRSSFSEGQTAPV
T382 SSFSEGQTAPVASGT
K391 PVASGTKKSHIRSHS
S396 TKKSHIRSHSDTNIA
S398 KSHIRSHSDTNIASR
S404 HSDTNIASRGAAEGG
- gap
- gap
Y413 GAAEGGQYLCSGEGM
S425 EGMFRRPSEGQSLIS
S429 RRPSEGQSLISYLSE
S484 EEVEEEDSDREIQEL
S518 YRETENGSFRVTSSS
S526 FRVTSSSSQFSSRDS
S529 TSSSSQFSSRDSTQL
S533 SQFSSRDSTQLSESG
S537 SRDSTQLSESGSAED
S539 DSTQLSESGSAEDAD
S541 TQLSESGSAEDADDL
S565 SAVSNGKSSFSQNLS
S568 SNGKSSFSQNLSHCF
N570 GKSSFSQNLSHCFLH
S626 IPDSLPISPDDGQHA
T664 FNVHPAPTRKIAVAK
T683 CAGCGIRTDPDYIKR
Y687 GIRTDPDYIKRLRYC
Y727 KWDFSKYYVSNFSKD
S729 DFSKYYVSNFSKDLL
S732 KYYVSNFSKDLLLKI
S806 TEDLHLYSLSDLTAT
T811 LYSLSDLTATKKGEL
S907 QSLESYLSDYEEEPT
  rubicon iso4  
- under review  
S32 VKDIRWLSPHSALHV
S137 MFARKQESPLLVTKS
S146 LLVTKSQSLTALPGS
S165 PASYAQHSYFGSSSS
Y166 ASYAQHSYFGSSSSL
S169 AQHSYFGSSSSLQSM
S172 SYFGSSSSLQSMPQS
S187 SHSSERRSTSFSLSG
T188 HSSERRSTSFSLSGP
S189 SSERRSTSFSLSGPS
S207 QEDRECLSPAETQTT
T213 LSPAETQTTPAPLPS
S305 DQEEGRQSQAGSVLR
S314 AGSVLRRSSFSEGQT
S315 GSVLRRSSFSEGQTA
S317 VLRRSSFSEGQTAPV
T321 SSFSEGQTAPVASGT
K330 PVASGTKKSHIRSHS
S335 TKKSHIRSHSDTNIA
S337 KSHIRSHSDTNIASR
S343 HSDTNIASRGAAEGG
- gap
- gap
Y352 GAAEGGQYLCSGEGM
S364 EGMFRRPSEGQSLIS
S368 RRPSEGQSLISYLSE
S423 EEVEEEDSDREIQEL
S457 YRETENGSFRVTSSS
S465 FRVTSSSSQFSSRDS
S468 TSSSSQFSSRDSTQL
S472 SQFSSRDSTQLSESG
S476 SRDSTQLSESGSAED
S478 DSTQLSESGSAEDAD
S480 TQLSESGSAEDADDL
S504 SAVSNGKSSFSQNLS
S507 SNGKSSFSQNLSHCF
N509 GKSSFSQNLSHCFLH
S565 IPDSLPISPDDGQHA
T603 FNVHPAPTRKIAVAK
T622 CAGCGIRTDPDYIKR
Y626 GIRTDPDYIKRLRYC
Y666 KWDFSKYYVSNFSKD
S668 DFSKYYVSNFSKDLL
S671 KYYVSNFSKDLLLKI
S745 TEDLHLYSLSDLTAT
T750 LYSLSDLTATKKGEL
S846 QSLESYLSDYEEEPT
  rat

 
C44 EGLVSANCPNVWSKY
S92 VKDIRWLSPHSALHV
S197 MFARKQESPLLVTKS
S206 LLVTKSQSLTALPGS
S225 PTSCAQHSYFGSSSS
Y226 TSCAQHSYFGSSSSL
S229 AQHSYFGSSSSLHQS
S232 SYFGSSSSLHQSMPP
S247 SHSSERRSTSFSLSG
T248 HSSERRSTSFSLSGP
S249 SSERRSTSFSLSGPP
S267 QEDRECLSAAETQTT
T273 LSAAETQTTQAPLLS
S386 DQEAGRQSQAGSTLR
S395 AGSTLRRSSFSEGQT
S396 GSTLRRSSFSEGQTV
S398 TLRRSSFSEGQTVPA
T402 SSFSEGQTVPAASGT
K411 PAASGTKKSHIRSHS
S416 TKKSHIRSHSDTNIA
S418 KSHIRSHSDTNIASR
S424 HSDTNIASRGAAEGG
- gap
- gap
Y433 GAAEGGQYLCSGEGM
S445-p EGMFRRPsEGQSLIS
S449 RRPsEGQSLISYLSE
S504 EEVEEEDSDREIQEL
S538 YQETEHGSFRVTSSS
S546 FRVTSSSSQFSSRDS
S549 TSSSSQFSSRDSTQL
S553 SQFSSRDSTQLSDSG
S557 SRDSTQLSDSGSAED
S559 DSTQLSDSGSAEDVD
S561 TQLSDSGSAEDVDEF
S585 SMVSNGKSSFSQNFS
S588 SNGKSSFSQNFSHCF
N590 GKSSFSQNFSHCFLH
S646 IPDSLPISPDDGQHA
T684 FNVHPAPTRKIAVAK
T703 CAGCGIRTDPDYIKR
Y707 GIRTDPDYIKRLRYC
Y747 KWDFSKYYVSNFSKD
S749 DFSKYYVSNFSKDLL
S752 KYYVSNFSKDLLIKI
S826 TEDLHLYSLNDLTAA
T831 LYSLNDLTAAKKGEL
S927 QSLESYLSDYEEEPT
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