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Protein Page:
p70S6K (fruit fly)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
p70S6K an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, AGC; EC 2.7.11.1; Translation; Kinase, protein; EC 2.7.11.-; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; p70 subfamily
Cellular Component: presynaptic active zone; ribosome
Molecular Function: protein serine/threonine kinase activity; protein binding; ribosomal protein S6 kinase activity; ATP binding
Biological Process: oogenesis; axon guidance; positive regulation of cell size; multicellular organism growth; dendrite morphogenesis; cellular response to stress; actin filament organization; determination of adult life span; positive regulation of multicellular organism growth; signal transduction; positive regulation of cell growth; protein amino acid phosphorylation; positive regulation of organ growth; somatic muscle development; phagocytosis; synaptic growth at neuromuscular junction; lipid metabolic process; positive regulation of growth; regulation of cell size; response to DNA damage stimulus; positive regulation of macroautophagy; larval feeding behavior; response to nutrient; myoblast fusion; positive regulation of autophagy; growth
Reference #:  NP_523941 (RefSeq)
Alt. Names/Synonyms: P70s6k protein kinase homolog; RPS6-p70-protein kinase; S6k; S6K1; s6k11; Tor
Gene Symbols: S6k
Molecular weight: 55,427 Da
Basal Isoelectric point: 5.66  Predict pI for various phosphorylation states
Select Structure to View Below

p70S6K

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

STRING  |  Scansite  |  Pfam  |  ENZYME  |  Phospho.ELM  |  UniProtKB  |  Entrez-Gene  |  GenPept


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       fruit fly

 
2 6 - gap
1 0 - gap
1 0 - gap
0 1 K109 ANKYFAMKVLKKASI
0 1 K112 YFAMKVLKKASIVTN
0 1 T126 NQKDTAHTRAERNIL
0 2 R203 HKLGIIYRDLKPENI
0 6 H229 DFGLCKEHIQEGIVT
0 5 I234 KEHIQEGIVTHTFCG
0 12 T236 HIQEGIVTHTFCGTI
27 18 T238 QEGIVTHTFCGTIEY
0 6 T242 VTHTFCGTIEYMAPE
0 1 T290 NRKKTIETILKAKLN
2 11 I376 DTRFTRQIPVDSPDD
11 9 S380 TRQIPVDSPDDTTLS
1 1 S389 DDTTLSESANLIFQG
308 3 T398 NLIFQGFTYVAPSIL
0 2 Y399 LIFQGFTYVAPSILE
0 1 S403 GFTYVAPSILEDMHR
0 2 D407 VAPSILEDMHRANRM
5 3 R413 EDMHRANRMPARSPR
25 1 S418 ANRMPARSPRRTPRQ
8 103 Q425 SPRRTPRQLPDSSFR
94 124 S429 TPRQLPDSSFRLQFP
90 257 S430 PRQLPDSSFRLQFPS
1 83 S437 SFRLQFPSANVGANA
1 0 S455 MHGHSQRSGMFARAT
1 0 - gap
1 0 - gap
2 0 - gap
9209 : Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody
  human

► Hide Isoforms
 
S40-p DQPEDAGsEDELEEG
S53-p EGGQLNEsMDHGGVG
Y62 DHGGVGPYELGMEHC
K123 TGKIFAMKVLKKAMI
K126 IFAMKVLKKAMIVRN
T140 NAKDTAHTKAERNIL
R217 HQKGIIYRDLKPENI
S243-p DFGLCKEsIHDGtVt
T248-p KEsIHDGtVtHtFCG
T250-p sIHDGtVtHtFCGtI
T252-p HDGtVtHtFCGtIEY
T256-p VtHtFCGtIEYMAPE
K304-ac NRKKTIDkILKCKLN
T390-p DSKFTRQtPVDsPDD
S394-p TRQtPVDsPDDSTLS
S403-p DDSTLSEsANQVFLG
T412-p NQVFLGFtyVAPsVL
Y413-p QVFLGFtyVAPsVLE
S417-p GFtyVAPsVLEsVKE
S421-p VAPsVLEsVKEKFsF
S427-p EsVKEKFsFEPKIRs
S434-p sFEPKIRsPRRFIGs
S441-p sPRRFIGsPRtPVsP
T444-p RFIGsPRtPVsPVKF
S447-p GsPRtPVsPVKFsPG
S452-p PVsPVKFsPGDFWGR
T470-p ASTANPQtPVEYPME
K507 QPNSGPYKKQAFPMI
K508 PNSGPYKKQAFPMIS
K516-ac QAFPMISkRPEHLRM
9208 : Phospho-p70 S6 Kinase (Ser371) Antibody
9205 : Phospho-p70 S6 Kinase (Thr389) Antibody
9206 : Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb
9209 : Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody
9234 : Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
  p70S6K iso2  
S17 DQPEDAGSEDELEEG
S30 EGGQLNESMDHGGVG
Y39 DHGGVGPYELGMEHC
K100 TGKIFAMKVLKKAMI
K103 IFAMKVLKKAMIVRN
T117 NAKDTAHTKAERNIL
R194 HQKGIIYRDLKPENI
S220 DFGLCKESIHDGTVT
T225 KESIHDGTVTHtFCG
T227 SIHDGTVTHtFCGTI
T229-p HDGTVTHtFCGTIEY
T233 VTHtFCGTIEYMAPE
K281 NRKKTIDKILKCKLN
T367 DSKFTRQTPVDSPDD
S371 TRQTPVDSPDDSTLS
S380 DDSTLSESANQVFLG
T389-p NQVFLGFtYVAPSVL
Y390 QVFLGFtYVAPSVLE
S394 GFtYVAPSVLESVKE
S398 VAPSVLESVKEKFSF
S404 ESVKEKFSFEPKIRS
S411 SFEPKIRSPRRFIGS
S418 SPRRFIGSPRtPVsP
T421-p RFIGSPRtPVsPVKF
S424-p GSPRtPVsPVKFSPG
S429 PVsPVKFSPGDFWGR
T447 ASTANPQTPVEYPME
K484-ac QPNSGPYkkQAFPMI
K485-ac PNSGPYkkQAFPMIS
K493-ac QAFPMISkRPEHLRM
9209 : Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody
  mouse

 
S40 DQPEDAGSEDELEEG
S53 EGGQLNESMDHGGVG
Y62 DHGGVGPYELGMEHC
K123-ac TGKIFAMkVLkKAMI
K126-ac IFAMkVLkKAMIVRN
T140-p NAKDTAHtKAERNIL
R217-m1 HQKGIIYrDLKPENI
S243-p DFGLCKEsIHDGtVt
T248-p KEsIHDGtVtHtFCG
T250-p sIHDGtVtHtFCGtI
T252-p HDGtVtHtFCGtIEY
T256-p VtHtFCGtIEYMAPE
K304 NRKKTIDKILKCKLN
T390-p DSKFTRQtPVDsPDD
S394-p TRQtPVDsPDDSTLS
S403 DDSTLSESANQVFLG
T412-p NQVFLGFtYVAPSVL
Y413 QVFLGFtYVAPSVLE
S417 GFtYVAPSVLESVKE
S421 VAPSVLESVKEKFsF
S427-p ESVKEKFsFEPKIRs
S434-p sFEPKIRsPRRFIGs
S441-p sPRRFIGsPRtPVsP
T444-p RFIGsPRtPVsPVKF
S447-p GsPRtPVsPVKFsPG
S452-p PVsPVKFsPGDFWGR
T470 ASTANPQTPVEYPME
K507 QPNSGPYKKQAFPMI
K508 PNSGPYKKQAFPMIS
K516 QAFPMISKRPEHLRM
9208 : Phospho-p70 S6 Kinase (Ser371) Antibody
9205 : Phospho-p70 S6 Kinase (Thr389) Antibody
9206 : Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb
9234 : Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
  rat

► Hide Isoforms
 
S40-p DQPEDAGsEDELEEG
S53 EGGQLNESMDHGGVG
Y62-p DHGGVGPyELGMEHC
K123 TGKIFAMKVLKKAMI
K126 IFAMKVLKKAMIVRN
T140 NAKDTAHTKAERNIL
R217 HQKGIIYRDLKPENI
S243 DFGLCKESIHDGTVT
T248 KESIHDGTVTHtFCG
T250 SIHDGTVTHtFCGTI
T252-p HDGTVTHtFCGTIEY
T256 VTHtFCGTIEYMAPE
K304-ac NRKKTIDkILKCKLN
T390 DSKFTRQTPVDsPDD
S394-p TRQTPVDsPDDSTLS
S403 DDSTLSESANQVFLG
T412-p NQVFLGFtYVAPSVL
Y413 QVFLGFtYVAPSVLE
S417 GFtYVAPSVLESVKE
S421 VAPSVLESVKEKFSF
S427 ESVKEKFSFEPKIRs
S434-p SFEPKIRsPRRFIGs
S441-p sPRRFIGsPRtPVsP
T444-p RFIGsPRtPVsPVKF
S447-p GsPRtPVsPVKFsPG
S452-p PVsPVKFsPGDFWGR
T470 ASTANPQTPVEYPME
K507 QPNSGPYKKQAFPMI
K508 PNSGPYKKQAFPMIS
K516 QAFPMISKRPEHLRM
9208 : Phospho-p70 S6 Kinase (Ser371) Antibody
9205 : Phospho-p70 S6 Kinase (Thr389) Antibody
9206 : Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb
9234 : Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
9204 : Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody
  p70S6K iso2  
S17-p DQPEDAGsEDELEEG
S30 EGGQLNESMDHGGVG
Y39 DHGGVGPYELGMEHC
K100 TGKIFAMKVLKKAMI
K103 IFAMKVLKKAMIVRN
T117 NAKDTAHTKAERNIL
R194 HQKGIIYRDLKPENI
S220 DFGLCKESIHDGTVT
T225 KESIHDGTVTHTFCG
T227 SIHDGTVTHTFCGTI
T229 HDGTVTHTFCGTIEY
T233 VTHTFCGTIEYMAPE
K281 NRKKTIDKILKCKLN
T367 DSKFTRQTPVDSPDD
S371 TRQTPVDSPDDSTLS
S380 DDSTLSESANQVFLG
T389-p NQVFLGFtYVAPSVL
Y390 QVFLGFtYVAPSVLE
S394 GFtYVAPSVLESVKE
S398 VAPSVLESVKEKFSF
S404 ESVKEKFSFEPKIRS
S411 SFEPKIRSPRRFIGS
S418 SPRRFIGSPRtPVsP
T421-p RFIGSPRtPVsPVKF
S424-p GSPRtPVsPVKFSPG
S429 PVsPVKFSPGDFWGR
T447 ASTANPQTPVEYPME
K484 QPNSGPYKKQAFPMI
K485 PNSGPYKKQAFPMIS
K493 QAFPMISKRPEHLRM
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