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Protein Page:
AMPKB1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
AMPKB1 a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity. Hematopoietic AMPKB1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity. Note: This description may include information from UniProtKB.
Protein type: Autophagy; Protein kinase, regulatory subunit
Cellular Component: cytosol; nucleus; AMP-activated protein kinase complex
Molecular Function: protein binding; protein kinase binding; protein kinase activity
Biological Process: regulation of catalytic activity; protein heterooligomerization; insulin receptor signaling pathway; cell cycle arrest; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process
Reference #:  Q9Y478 (UniProtKB)
Alt. Names/Synonyms: 5'-AMP-activated protein kinase beta-1 subunit; 5'-AMP-activated protein kinase subunit beta-1; AAKB1; AMP-activated protein kinase beta subunit; AMPK; AMPK beta -1 chain; AMPK beta 1; AMPK subunit beta-1; AMPKb; HAMPKb; MGC17785; PRKAB1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1
Gene Symbols: PRKAB1
Molecular weight: 30,382 Da
Basal Isoelectric point: 5.94  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AMPKB1

Protein Structure Not Found.


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Sites Implicated In
activity, induced: S108‑p
molecular association, regulation: S108‑p
phosphorylation: S108‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T4-p ____MGNtssERAAL
0 1 S5-p ___MGNtssERAALE
0 1 S6-p __MGNtssERAALER
0 15 T19-p ERHGGHKtPRRDssG
4 14 S24-p HKtPRRDssGGTKDG
3 5 S25-p KtPRRDssGGTKDGD
0 7 S40-p RPKILMDsPEDADLF
0 1 K57-u EEIKAPEkEEFLAWQ
0 1 K72 HDLEVNDKAPAQARP
1 0 T80-p APAQARPtVFRWTGG
0 4 Y92-p TGGGKEVyLSGsFNN
0 8 S96-p KEVyLSGsFNNWsKL
1 2 S101-p SGsFNNWsKLPLTRs
0 1 T106 NWsKLPLTRsHNNFV
8 35 S108-p sKLPLTRsHNNFVAI
0 3 S144-p PSEPIVTsQLGtVNN
0 2 T148-p IVTsQLGtVNNIIQV
1 0 T158-p NIIQVKKtDFEVFDA
1 1 S174-p MVDSQKCsDVsELss
1 0 S177-p SQKCsDVsELsssPP
0 1 S180-p CsDVsELsssPPGPy
0 1 S181-p sDVsELsssPPGPyH
2 9 S182-p DVsELsssPPGPyHQ
0 7 Y187-p sssPPGPyHQEPYVC
0 1 T254-p GVMVLSAtHRYKKKY
4181 : Phospho-AMPKbeta1 (Ser108) Antibody
4186 : Phospho-AMPKbeta1 (Ser182) Antibody
  mouse

 
T4 ____MGNTSSERAAL
S5 ___MGNTSSERAALE
S6 __MGNTSSERAALER
T19 ERQAGHKTPRRDssG
S24-p HKTPRRDssGGAKDG
S25-p KTPRRDssGGAKDGD
S40-p RPKILMDsPEDADIF
K57 EEIKAPEKEEFLAWQ
K72-u HDLEANDkAPAQARP
T80 APAQARPTVFRWTGG
Y92 TGGGKEVYLSGsFNN
S96-p KEVYLSGsFNNWsKL
S101-p SGsFNNWsKLPLtRs
T106-p NWsKLPLtRsQNNFV
S108-p sKLPLtRsQNNFVAI
S144-p PSEPIVTsQLGTVNN
T148 IVTsQLGTVNNIIQV
T158 NIIQVKKTDFEVFDA
S174-p MVDSQKCsDVsELss
S177-p SQKCsDVsELsssPP
S180-p CsDVsELsssPPGPY
S181-p sDVsELsssPPGPYH
S182-p DVsELsssPPGPYHQ
Y187 sssPPGPYHQEPYMS
T254 GVMVLSATHRYKKKY
4181 : Phospho-AMPKbeta1 (Ser108) Antibody
4186 : Phospho-AMPKbeta1 (Ser182) Antibody
  rat

 
T4 ____MGNTSSERAAL
S5 ___MGNTSSERAALE
S6 __MGNTSSERAALER
T19 ERQAGHKTPRRDssG
S24-p HKTPRRDssGGTKDG
S25-p KTPRRDssGGTKDGD
S40 RPKILMDSPEDADIF
K57 EEMKAPEKEEFLAWQ
K72 HDLEVNEKAPAQARP
T80 APAQARPTVFRWTGG
Y92 TGGGKEVYLSGsFNN
S96-p KEVYLSGsFNNWsKL
S101-p SGsFNNWsKLPLTRs
T106 NWsKLPLTRsQNNFV
S108-p sKLPLTRsQNNFVAI
S144 PSEPIVTSQLGTVNN
T148 IVTSQLGTVNNIIQV
T158 NIIQVKKTDFEVFDA
S174 MVDSQKCSDVSELSs
S177 SQKCSDVSELSssPP
S180 CSDVSELSssPPGPY
S181-p SDVSELSssPPGPYH
S182-p DVSELSssPPGPYHQ
Y187 SssPPGPYHQEPYIS
T254 GVMVLSATHRYKKKY
4186 : Phospho-AMPKbeta1 (Ser182) Antibody
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