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Protein Page:
ASH2L (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ASH2L Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. Interacts with HCFC1. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, KAT8/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with DPY30 and RBBP5; the interaction is direct. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with SETD1A and SETD1B. Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Methyltransferase; Transcription regulation
Cellular Component: histone methyltransferase complex; nucleus
Molecular Function: protein binding; DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); metal ion binding
Biological Process: transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to estrogen stimulus; positive regulation of cell proliferation; histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; response to DNA damage stimulus
Reference #:  Q9UBL3 (UniProtKB)
Alt. Names/Synonyms: ASH2; ash2 (absent, small, or homeotic)-like (Drosophila); ASH2-like protein; ASH2L; ASH2L1; ASH2L2; Bre2; Set1/Ash2 histone methyltransferase complex subunit ASH2
Gene Symbols: ASH2L
Molecular weight: 68,723 Da
Basal Isoelectric point: 5.45  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ASH2L

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 T84-p ESSNGKDtLEGAGDT
0 4 S101-p VMDTQAGsVDEENGR
0 1 Y204-p IIPFIDKyWECMTTR
0 3 S316-p GTTKKARsDPLFsAQ
0 1 S321-p ARsDPLFsAQRLPPH
0 1 K338-u PLEHPFNkDGYRYIL
0 1 Y407-p TVVGEKGySMVRASH
0 1 S459-p GYDKFSYsWRSKKGT
0 1 S516-p KALIKFKsyLyFEEK
0 7 Y517-p ALIKFKsyLyFEEKD
0 8 Y519-p IKFKsyLyFEEKDFV
0 5 S623-p TEVDGRRsPPWEP__
  mouse

 
T79 ESSNGKDTLEGTGDT
S96-p VMDTQAGsVDEENGR
Y199 IIPFIDKYWECMTTR
S311 GTTKKARSDPLFSAQ
S316 ARSDPLFSAQRLPPH
K333 PLEHPFNKDGYRYIL
Y402 TVVGEKGYSMVRASH
S454 GYDKFSYSWRSKKGT
S511 KALIKFKSYLYFEEK
Y512 ALIKFKSYLYFEEKD
Y514 IKFKSYLYFEEKDFV
S618-p TEVDGRRsPPWEP__
  rat

 
T79 ESSNGKDTLEGAGDT
S96 VMDTQAGSVDEENGR
Y199 IIPFIDKYWECMTTR
S311 GTTKKARSDPLFSAQ
S316 ARSDPLFSAQRLPPH
K333 PLEHPFNKDGYRYIL
Y402 TVVGEKGYSMVRASH
S454 GYDKFSYSWRSKKGT
S511 KALIKFKSYLYFEEK
Y512 ALIKFKSYLYFEEKD
Y514 IKFKSYLYFEEKDFV
S618-p TEVDGRRsPPWEP__
  fruit fly

 
- gap
- gap
Y156 IIPYIEQYWEAMTTM
S260 GKKGRPSSDITANVK
A264 RPSSDITANVKLPPH
K281 PLEHPFNKDGYRYIL
Y351 AVTGERGYCMVRATH
S403 GYDKFGYSWRSRKGT
S459 RPLVKFKSHLYYEDK
H460 PLVKFKSHLYYEDKD
Y462 VKFKSHLYYEDKDKI
- gap
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