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Protein Page:
USP4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
USP4 Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Ubiquitin conjugating system; EC 3.1.2.15; Protease
Cellular Component: lysosome; cytoplasm; plasma membrane; nucleus
Molecular Function: ubiquitin thiolesterase activity; identical protein binding; protein binding; metal ion binding; ubiquitin-specific protease activity; adenosine receptor binding
Biological Process: ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of protein stability; negative regulation of protein ubiquitination
Reference #:  Q13107 (UniProtKB)
Alt. Names/Synonyms: Deubiquitinating enzyme 4; MGC149848; MGC149849; ubiquitin carboxyl-terminal esterase 4; Ubiquitin carboxyl-terminal hydrolase 4; ubiquitin specific peptidase 4 (proto-oncogene); ubiquitin specific protease 4; ubiquitin specific protease 4 (proto-oncogene); Ubiquitin thioesterase 4; ubiquitin thiolesterase 4; ubiquitin-specific processing protease 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog; UBP4; UNP; UNPH; USP4
Gene Symbols: USP4
Molecular weight: 108,565 Da
Basal Isoelectric point: 5.47  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

USP4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y136-p KHCKVEVyLLELKLC
0 2 K186-ub RETRLWNkYMSNTyE
0 6 Y192-p NkYMSNTyEQLsKLD
0 1 S196-p SNTyEQLsKLDNTVQ
0 1 K232 PRQTLQSKSSTAPsR
0 1 S238-p SKSSTAPsRNFTTsP
0 1 S244-p PsRNFTTsPKSSAsP
0 1 S250-p TsPKSSAsPySSVSA
0 2 Y252-p PKSSAsPySSVSASL
0 1 K348-ub RDNPLGMkGEIAEAY
0 1 S364-p ELIKQMWsGRDAHVA
0 1 K376 HVAPRMFKTQVGRFA
1 2 S445-p NHRLRNDsVIVDTFH
0 1 T458-p FHGLFKStLVCPECA
0 1 Y476 VTFDPFCYLTLPLPL
0 1 Y539-p NMVVADVyNHRFHKI
0 1 K588 PVYFRERKSRPSSTs
0 1 S595-p KSRPSSTsSAsALYG
0 1 S598-p PSSTsSAsALYGQPL
0 6 S675-p EEGKEQLsETEGsGE
0 7 S680-p QLsETEGsGEDEPGN
0 1 K696 PSETTQKKIKGQPCP
0 1 A725 ADINSLAADGkLLKL
0 1 K728-ub NSLAADGkLLKLNSR
0 1 K731 AADGkLLKLNSRSTL
0 1 Y750-p DSETRRLyyDEQEsE
0 1 Y751-p SETRRLyyDEQEsEA
0 1 S756-p LyyDEQEsEAyEKHV
0 1 Y759-p DEQEsEAyEKHVSML
0 1 K770 VSMLQPQKKKKTTVA
0 2 K811-ub KHQQATKkFDLWSLP
0 1 S816 TKkFDLWSLPKILVV
0 4 K837-ub YNRYWRDkLDTVVEF
  mouse

 
Y136 KHCKVEVYLLELKLC
K186-ub RETRLWNkYMSNTyE
Y192-p NkYMSNTyEQLSKLD
S196 SNTyEQLSKLDNTIQ
K232-ub PRQSLQSkSSTAPSR
S238 SkSSTAPSRNFTTSS
S244 PSRNFTTSSKPSASP
S250 TSSKPSASPYCSVSA
Y252 SKPSASPYCSVSASL
K348 RDNPLGMKGEIAEAY
S364 ELIKQMWSGRDTHVA
K376-ub HVAPRMFkTQVGRFA
S445-p NHRLRNDsVIVDTFH
T458 FHGLFKSTLVCPECA
Y476-p VTFDPFCyLTLPLPL
Y539 NMVVTDVYNHRFHKI
K588-ub PVYFREKkSRPSSAS
S595 kSRPSSASSGAVLYG
A598 PSSASSGAVLYGQPL
S675-p EEGKEQLsEVEGsGE
S680-p QLsEVEGsGEDDQGD
K695-ub DHSESAQkVKGQPRH
T724-p ADINSLAtDGKLLkL
K727 NSLAtDGKLLkLNSR
K730-ub AtDGKLLkLNSRSTL
Y749 DSETRSLYFDEQESE
F750 SETRSLYFDEQESEA
S755 LYFDEQESEACEKHL
C758 DEQESEACEKHLSMS
K769-ub LSMSQPQkKKKAAVA
K810-ub KHQQATKkFDLWsLP
S815-p TKkFDLWsLPKILVV
K836-ub YNRYWRDkLDTVVEF
  rat

 
Y136 KHCKVEVYLLELKLC
K186 RETRLWNKYMSNTYE
Y192 NKYMSNTYEQLSKLD
S196 SNTYEQLSKLDNTIQ
K232 PRQTLQSKSSTAPSR
S238 SKSSTAPSRNFTTSS
S244 PSRNFTTSSKPSASP
S250 TSSKPSASPYSSMSA
Y252 SKPSASPYSSMSASL
K348 RDNPLGMKGEIAEAY
S364 ELIKQMWSGRDTHVA
K376 HVAPRMFKTQVGRFA
S445 NHRLRNDSVIVDTFH
T458 FHGLFKSTLVCPECA
Y476 VTFDPFCYLTLPLPL
Y539 NMVVTDVYNHRFHKI
K588 PVYFREKKSRPSSTS
S595 KSRPSSTSSGAVLYG
A598 PSSTSSGAVLYGQPL
S674 EEGKEQLSEVEESGE
S679 QLSEVEESGEDSQGG
K694 DPTETTQKAKGPPRH
T723 ADINSLATDGKLLKL
K726 NSLATDGKLLKLNSR
K729 ATDGKLLKLNSRSTL
Y748 DSETRSLYFDEQESE
F749 SETRSLYFDEQESEA
S754 LYFDEQESEACEKHT
C757 DEQESEACEKHTSMS
K768 TSMSQPQKKKKAAIA
K809 KHQQATKKFDLWSLP
S814 TKKFDLWSLPKILVV
K835 YNRYWRDKLDTVVEF
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