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USP4 (human)

Overview USP4 Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Ubiquitin conjugating system; EC 3.4.19.12; EC 3.1.2.15; Protease; Ubiquitin-specific protease Cellular Component: lysosome; cytoplasm; nucleus Molecular Function: ubiquitin thiolesterase activity; protein binding; metal ion binding; ubiquitin-specific protease activity; adenosine receptor binding Biological Process: ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of protein stability; negative regulation of protein ubiquitination Reference #:  Q13107 (UniProtKB) Alt. Names/Synonyms: Deubiquitinating enzyme 4; MGC149848; MGC149849; ubiquitin carboxyl-terminal esterase 4; Ubiquitin carboxyl-terminal hydrolase 4; ubiquitin specific peptidase 4 (proto-oncogene); ubiquitin specific protease 4; ubiquitin specific protease 4 (proto-oncogene); Ubiquitin thioesterase 4; ubiquitin thiolesterase 4; ubiquitin-specific processing protease 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog; UBP4; UNP; UNPH; USP4 Gene Symbols: USP4 Molecular weight: 108,565 Da Basal Isoelectric point: 5.47  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below USP4 2Y6E - F/A/D/C/E/B=765-932 (human)

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		Y136-p	KHCKVEVyLLELKLC
0	2		K186-u	RETRLWNkYMSNTyE
0	6		Y192-p	NkYMSNTyEQLsKLD
0	1		S196-p	SNTyEQLsKLDNTVQ
0	1		K232	PRQTLQSKSSTAPsR
0	1		S238-p	SKSSTAPsRNFTTSP
0	2		Y252-p	PKSSASPySSVSASL
0	1		K348-u	RDNPLGMkGEIAEAY
0	1		S364-p	ELIKQMWsGRDAHVA
0	1		K376	HVAPRMFKTQVGRFA
1	2		S445-p	NHRLRNDsVIVDTFH
0	1		T458-p	FHGLFKStLVCPECA
0	1		Y476-p	VTFDPFCyLTLPLPL
0	1		Y539-p	NMVVADVyNHRFHKI
0	1		K588	PVYFRERKSRPSSTs
0	1		S595-p	KSRPSSTsSAsALYG
0	1		S598-p	PSSTsSAsALYGQPL
0	3		S675-p	EEGKEQLsETEGsGE
0	4		S680-p	QLsETEGsGEDEPGN
0	1		K696	PSETTQKKIKGQPCP
0	1		A725	ADINSLAADGkLLKL
0	1		K728-u	NSLAADGkLLKLNSR
0	1		K731	AADGkLLKLNSRSTL
0	1		K770	VSMLQPQKKKKTTVA
0	2		K811-u	KHQQATKkFDLWSLP
0	1		S816	TKkFDLWSLPKILVV
0	4		K837-u	YNRYWRDkLDTVVEF

	mouse
Y136	KHCKVEVYLLELKLC
K186-u	RETRLWNkYMSNTyE
Y192-p	NkYMSNTyEQLSKLD
S196	SNTyEQLSKLDNTIQ
K232-u	PRQSLQSkSSTAPSR
S238	SkSSTAPSRNFTTSS
Y252	SKPSASPYCSVSASL
K348	RDNPLGMKGEIAEAY
S364	ELIKQMWSGRDTHVA
K376-u	HVAPRMFkTQVGRFA
S445-p	NHRLRNDsVIVDTFH
T458	FHGLFKSTLVCPECA
Y476-p	VTFDPFCyLTLPLPL
Y539	NMVVTDVYNHRFHKI
K588-u	PVYFREKkSRPSSAS
S595	kSRPSSASSGAVLYG
A598	PSSASSGAVLYGQPL
S675-p	EEGKEQLsEVEGsGE
S680-p	QLsEVEGsGEDDQGD
K695-u	DHSESAQkVKGQPRH
T724-p	ADINSLAtDGKLLkL
K727	NSLAtDGKLLkLNSR
K730-u	AtDGKLLkLNSRSTL
K769-u	LSMSQPQkKKKAAVA
K810-u	KHQQATKkFDLWsLP
S815-p	TKkFDLWsLPKILVV
K836-u	YNRYWRDkLDTVVEF

	rat
Y136	KHCKVEVYLLELKLC
K186	RETRLWNKYMSNTYE
Y192	NKYMSNTYEQLSKLD
S196	SNTYEQLSKLDNTIQ
K232	PRQTLQSKSSTAPSR
S238	SKSSTAPSRNFTTSS
Y252	SKPSASPYSSMSASL
K348	RDNPLGMKGEIAEAY
S364	ELIKQMWSGRDTHVA
K376	HVAPRMFKTQVGRFA
S445	NHRLRNDSVIVDTFH
T458	FHGLFKSTLVCPECA
Y476	VTFDPFCYLTLPLPL
Y539	NMVVTDVYNHRFHKI
K588	PVYFREKKSRPSSTS
S595	KSRPSSTSSGAVLYG
A598	PSSTSSGAVLYGQPL
S674	EEGKEQLSEVEESGE
S679	QLSEVEESGEDSQGG
K694	DPTETTQKAKGPPRH
T723	ADINSLATDGKLLKL
K726	NSLATDGKLLKLNSR
K729	ATDGKLLKLNSRSTL
K768	TSMSQPQKKKKAAIA
K809	KHQQATKKFDLWSLP
S814	TKKFDLWSLPKILVV
K835	YNRYWRDKLDTVVEF

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