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Protein Page:
PRC1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PRC1 translocates to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. Acts as a microtubule-binding and bundling protein both in vivo and vitro. Interacts with the C-terminal Rho-GAP domain and the basic region of MgcRacGAP. The interaction with MgcRacGAP inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. Note: This description may include information from UniProtKB.
Protein type: Microtubule binding protein; Motility/polarity/chemotaxis; Cytoskeletal protein; Cell cycle regulation
Chromosomal Location of Human Ortholog: 15q26.1
Cellular Component: microtubule cytoskeleton; spindle pole; spindle microtubule; cytoplasm; nucleolus; plasma membrane; spindle; nucleus
Molecular Function: identical protein binding; protein binding; microtubule binding; protein kinase binding
Biological Process: mitotic spindle elongation; cytokinesis
Reference #:  O43663 (UniProtKB)
Alt. Names/Synonyms: anaphase spindle elongation 1 homolog; ASE1; MGC1671; MGC3669; PRC1; protein regulating cytokinesis 1; Protein regulator of cytokinesis 1
Gene Symbols: PRC1
Molecular weight: 71,607 Da
Basal Isoelectric point: 6.29  Predict pI for various phosphorylation states
CST Pathways:  Histone Methylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PRC1

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: T470‑p, T481‑p
cytoskeletal reorganization: T470‑p, T481‑p
intracellular localization: T470‑p
molecular association, regulation: S615‑p, T616‑p
phosphorylation: S577‑p, T578‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 5 S4-p ____MRRsEVLAEES
0 1 S11 sEVLAEESIVCLQkA
0 3 K17-ub ESIVCLQkALNHLRE
0 1 T105-p QLEKDLRtQVELMRK
0 1 S195-p LDHTPDTsFERDVVC
0 1 T262-p EEREAVAtIMsGSKA
0 1 S265-p EAVAtIMsGSKAKVR
0 1 T379-p ASDPNRFtNRGGNLL
0 1 K453-ac AKQERQLkNKKQTET
0 52 Y464-p QTETEMLyGsAPRtP
0 6 S466-p ETEMLyGsAPRtPsK
4 56 T470-p LyGsAPRtPsKRRGL
0 1 S472-p GsAPRtPsKRRGLAP
4 16 T481-p RRGLAPNtPGKARKL
0 1 S500 MSNATANSSIRPIFG
0 1 T509-p IRPIFGGtVyHsPVs
0 7 Y511-p PIFGGtVyHsPVsRL
0 38 S513-p FGGtVyHsPVsRLPP
0 2 S516-p tVyHsPVsRLPPsGs
0 1 S521-p PVsRLPPsGsKPVAA
0 1 S523-p sRLPPsGsKPVAAsT
0 1 S529-p GsKPVAAsTCsGkKt
0 2 S532-p PVAAsTCsGkKtPRT
0 12 K534-ac AAsTCsGkKtPRTGR
0 8 T536-p sTCsGkKtPRTGRHG
1 5 S554-p ENLELNGsILsGGyP
0 2 S557-p ELNGsILsGGyPGsA
0 1 Y560-p GsILsGGyPGsAPLQ
0 1 S563-p LsGGyPGsAPLQRNF
1 15 S571-p APLQRNFsINsVAst
1 1 S574-p QRNFsINsVAstYSE
1 0 S577-p FsINsVAstYSEFAK
1 0 T578-p sINsVAstYSEFAKD
0 8 S587-p SEFAKDPsLsDsstV
0 5 S589-p FAKDPsLsDsstVGL
0 4 S591-p KDPsLsDsstVGLQR
0 7 S592-p DPsLsDsstVGLQRE
0 1 T593-p PsLsDsstVGLQREL
0 1 K602-ub GLQRELSkASkSDAT
0 1 K605-ub RELSkASkSDATSGI
2 0 S615-p ATSGILNstNIQs__
4 1 T616-p TSGILNstNIQs___
0 2 S620-p LNstNIQs_______
  PRC1 iso2  
S4 ____MRRSEVLAEES
S11 SEVLAEESIVCLQKA
K17 ESIVCLQKALNHLRE
T105 QLEKDLRTQVELMRK
S195 LDHTPDTSFERDVVC
T262 EEREAVATIMSGSKA
S265 EAVATIMSGSKAKVR
T379 ASDPNRFTNRGGNLL
K423 AKQERQLKNKKQTET
Y434 QTETEMLYGSAPRtP
S436 ETEMLYGSAPRtPSK
T440-p LYGSAPRtPSKRRGL
S442 GSAPRtPSKRRGLAP
T451 RRGLAPNTPGKARKL
S470 MSNATANSSIRPIFG
T479 IRPIFGGTVYHSPVS
Y481 PIFGGTVYHSPVSRL
S483 FGGTVYHSPVSRLPP
S486 TVYHSPVSRLPPSGS
S491 PVSRLPPSGSKPVAA
S493 SRLPPSGSKPVAAST
S499 GSKPVAASTCSGKKt
S502 PVAASTCSGKKtPRT
K504 AASTCSGKKtPRTGR
T506-p STCSGKKtPRTGRHG
S524 ENLELNGSILSGGYP
S527 ELNGSILSGGYPGSA
Y530 GSILSGGYPGSAPLQ
S533 LSGGYPGSAPLQRNF
S541-p APLQRNFsINsVAST
S544-p QRNFsINsVASTYSE
S547 FsINsVASTYSEFAR
T548 sINsVASTYSEFARE
- gap
- gap
- gap
- gap
- gap
K558 EFARELSKASKSDAT
K561 RELSKASKSDATSGI
S571 ATSGILNSTNIQS__
T572 TSGILNSTNIQS___
S576 LNSTNIQS_______
  PRC1 iso3  
S4 ____MRRSEVLAEES
S11 SEVLAEESIVCLQKA
K17 ESIVCLQKALNHLRE
T64 QLEKDLRTQVELMRK
S154 LDHTPDTSFERDVVC
T221 EEREAVATIMSGSKA
S224 EAVATIMSGSKAKVR
T338 ASDPNRFTNRGGNLL
K412 AKQERQLKNKKQTET
Y423 QTETEMLYGSAPRTP
S425 ETEMLYGSAPRTPSK
T429 LYGSAPRTPSKRRGL
S431 GSAPRTPSKRRGLAP
T440 RRGLAPNTPGKARKL
S459 MSNATANSSIRPIFG
T468 IRPIFGGTVYHSPVS
Y470 PIFGGTVYHSPVSRL
S472 FGGTVYHSPVSRLPP
S475 TVYHSPVSRLPPSGS
S480 PVSRLPPSGSKPVAA
S482 SRLPPSGSKPVAAST
S488 GSKPVAASTCSGKKT
S491 PVAASTCSGKKTPRT
K493 AASTCSGKKTPRTGR
T495 STCSGKKTPRTGRHG
S513-p ENLELNGsILSARTF
S516 ELNGsILSARTFKGF
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
S4-p ____MRRsEVLADEs
S11-p sEVLADEsITCLQKA
K17 EsITCLQKALTHLRE
T105 QLEKDLRTQVELMRK
S195 LEHSPDTSFERDVVC
A262 EEREPVEAIMTGSKT
T265 EPVEAIMTGSKTKIR
T379 ASDPGRFTNRGGNLL
K453 AKQERQLKNKKQTEA
Y464 QTEAEMLYGSTPRTP
S466 EAEMLYGSTPRTPSK
T470 LYGSTPRTPSKRPGQ
S472 GSTPRTPSKRPGQTP
T478 PSKRPGQTPKKSGKM
S497-p MSSATPNsSIRPVFG
S506 IRPVFGGSVYRSPMS
Y508 PVFGGSVYRSPMSRL
S510 FGGSVYRSPMSRLPP
S513 SVYRSPMSRLPPSGS
S518 PMSRLPPSGSKSVVT
S520 SRLPPSGSKSVVTSL
S526 GSKSVVTSLCsGkKt
S529-p SVVTSLCsGkKtPRA
K531-ac VTSLCsGkKtPRAAQ
T533-p SLCsGkKtPRAAQLR
S551 ENLDLNGSILSGGYP
S554 DLNGSILSGGYPGST
Y557 GSILSGGYPGSTPLQ
S560 LSGGYPGSTPLQHNC
S568 TPLQHNCSIKSVAST
S571 QHNCSIKSVASTYSE
S574 CSIKSVASTYSEFSR
T575 SIKSVASTYSEFSRE
- gap
- gap
- gap
- gap
- gap
K585 EFSRELSKASRSDAT
R588 RELSKASRSDATSRI
S598 ATSRILNSTNIQS__
T599 TSRILNSTNIQS___
S603 LNSTNIQS_______
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