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Protein Page:
AML3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
AML3 Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: transcription factor complex; cytoplasm; nuclear chromatin; nucleolus; nucleus
Molecular Function: protein domain specific binding; protein binding; bHLH transcription factor binding; chromatin binding; transcription factor activity; ATP binding
Biological Process: embryonic forelimb morphogenesis; transcription initiation from RNA polymerase II promoter; ossification; positive regulation of transcription, DNA-dependent; cell maturation; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; embryonic cranial skeleton morphogenesis; stem cell differentiation; odontogenesis of dentine-containing teeth; osteoblast development; BMP signaling pathway; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; negative regulation of smoothened signaling pathway; positive regulation of cell proliferation; gene expression; negative regulation of transcription, DNA-dependent; T cell differentiation; regulation of odontogenesis of dentine-containing teeth; endochondral ossification; osteoblast fate commitment
Reference #:  Q13950 (UniProtKB)
Alt. Names/Synonyms: Acute myeloid leukemia 3 protein; AML3; CBF-alpha 1; CBF-alpha-1; CBFA1; CCD; CCD1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120022; MGC120023; Oncogene AML-3; OSF-2; OSF2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEA2aA; PEBP2-alpha A; PEBP2A; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; polyomavirus enhancer binding protein 2 alpha A subunit; Polyomavirus enhancer-binding protein 2 alpha A subunit; Runt-related transcription factor 2; RUNX2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
Gene Symbols: RUNX2
Molecular weight: 56,648 Da
Basal Isoelectric point: 9.03  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AML3

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: S465‑p
cell growth, altered: S465‑p
transcription, altered: S28‑p, S118‑p, S340‑p, S347‑p, S465‑p
transcription, induced: S196‑p, T198‑p, T200‑p, S294‑p, S312‑p
activity, inhibited: S118‑p
molecular association, regulation: S118‑p, S196‑p, T198‑p, T200‑p
protein degradation: S118‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 S24-p FFWDPSTsRRFsPPS
5 6 S28-p PSTsRRFsPPSSSLQ
0 1 S32 RRFsPPSSSLQPGKM
1 1 S43-p PGKMSDVsPVVAAQQ
3 0 S118-p AELVRTDsPNFLCSV
0 8 K176-ub RNASAVMkNQVARFN
1 0 S196-p GRSGRGKsFtLtITV
1 0 T198-p SGRGKsFtLtITVFT
1 0 T200-p RGKsFtLtITVFTNP
1 0 S237-p HRQKLDDsKPSLFSD
1 0 S240 KLDDsKPSLFSDRLs
1 0 S247-p SLFSDRLsDLGRIPH
0 1 R267 GVPPQNPRPSLNSAP
2 1 S275-p PSLNSAPsPFNPQGQ
6 1 S294-p DPRQAQSsPPWSYDQ
0 1 S302 PPWSYDQSYPSYLSQ
6 1 S312-p SYLSQMTsPsIHSTT
1 0 S314-p LSQMTsPsIHSTTPL
1 0 T319 sPsIHSTTPLSSTRG
2 4 S340-p TDVPRRIsDDDTATs
0 1 T344 RRIsDDDTATsDFCL
0 1 T346 IsDDDTATsDFCLWP
1 1 S347-p sDDDTATsDFCLWPS
0 1 S365 KKSQAGASELGPFSD
1 0 S388-p SLTESRFsNPRMHYP
0 1 R391 ESRFsNPRMHYPATF
4 0 S465-p MVPGGDRsPSRMLPP
1 0 S499-p DGVDADGsHSSsPTV
2 1 S503-p ADGsHSSsPTVLNSS
0 2 Y521-p DESVWRPy_______
  AML3 iso3  
S24 FFWDPSTSRRFSPPS
S28 PSTSRRFSPPSSSLQ
S32 RRFSPPSSSLQPGKM
S43 PGKMSDVSPVVAAQQ
S118 AELVRTDSPNFLCSV
K176 RNASAVMKNQVARFN
S196 GRSGRGKSFTLTITV
T198 SGRGKSFTLTITVFT
T200 RGKSFTLTITVFTNP
S237 HRQKLDDSKPSLFSD
S240 KLDDSKPSLFSDRLS
S247 SLFSDRLSDLGRIPH
R267 GVPPQNPRPSLNSAP
S275 PSLNSAPSPFNPQGQ
S294 DPRQAQSSPPWSYDQ
S302 PPWSYDQSYPSYLSQ
S312 SYLSQMTSPSIHSTT
S314 LSQMTSPSIHSTTPL
T319 SPSIHSTTPLSSTRG
S340-p TDVPRRIsGAsELGP
- gap
- gap
- gap
S343-p PRRIsGAsELGPFSD
S366 SLTESRFSNPRMHYP
R369 ESRFSNPRMHYPATF
S443 MVPGGDRSPSRMLPP
S477 DGVDADGSHSSSPTV
S481 ADGSHSSSPTVLNSS
Y499 DESVWRPY_______
  mouse

► Hide Isoforms
 
S103 FFWDPSTSRRFsPPS
S107-p PSTSRRFsPPSsSLQ
S111-p RRFsPPSsSLQPGKM
S122 PGKMSDVSPVVAAQQ
S204-p AELVRTDsPNFLCSV
K262 RNASAVMKNQVARFN
S282 GRSGRGKSFTLTITV
T284 SGRGKSFTLTITVFT
T286 RGKSFTLTITVFTNP
S323 HRQKLDDSKPsLFSD
S326-p KLDDSKPsLFSDRLS
S333 sLFSDRLSDLGRIPH
R353-m2 GVPPQNPrPSLNSAP
S361-p PSLNSAPsPFNPQGQ
S380-p DPRQAQSsPPWSYDQ
S388-p PPWSYDQsYPSYLSQ
S398-p SYLSQMTsPSIHSTT
S400 LSQMTsPSIHSTTPL
T405 sPSIHSTTPLSSTRG
S426-p TDVPRRIsDDDtAtS
T430-p RRIsDDDtAtSDFCL
T432-p IsDDDtAtSDFCLWP
S433 sDDDtAtSDFCLWPS
S451 KKSQAGASELGPFSD
S474 SLTESRFSNPrMHYP
R477-m1 ESRFSNPrMHYPATF
S551-p MVPGGDRsPSRMVPP
S585 DGVDADGSHSSSPTV
S589 ADGSHSSSPTVLNSS
Y607 DESVWRPY_______
  AML3 iso5  
S24 FFWDPSTSRRFSPPS
S28 PSTSRRFSPPSSSLQ
S32 RRFSPPSSSLQPGKM
S43 PGKMSDVSPVVAAQQ
S125 AELVRTDSPNFLCSV
K183 RNASAVMKNQVARFN
S203 GRSGRGKSFTLTITV
T205 SGRGKSFTLTITVFT
T207 RGKSFTLTITVFTNP
S244 HRQKLDDSKPSLFSD
S247 KLDDSKPSLFSDRLS
S254 SLFSDRLSDLGRIPH
R274 GVPPQNPRPSLNSAP
S282 PSLNSAPSPFNPQGQ
S301-p DPRQAQSsPPWSYDQ
S309 PPWSYDQSYPSYLSQ
S319-p SYLSQMTsPSIHSTt
S321 LSQMTsPSIHSTtPL
T326-p sPSIHSTtPLSSTRG
S347 TDVPRRISDDDTATS
T351 RRISDDDTATSDFCL
T353 ISDDDTATSDFCLWP
S354 SDDDTATSDFCLWPS
S372 KKSQAGASELGPFSD
S395 SLTESRFSNPRMHYP
R398 ESRFSNPRMHYPATF
S472 MVPGGDRSPSRMVPP
S506 DGVDADGSHSSSPTV
S510 ADGSHSSSPTVLNSS
Y528 DESVWRPY_______
  rat

 
S24 FFWDPSTSRRFSPPS
S28 PSTSRRFSPPSSSLQ
S32 RRFSPPSSSLQPGKM
S43 PGKMSDVSPVVAAQQ
S126 AELVRTDSPNFLCSV
K184 RNASAVMKNQVARFN
S204 GRSGRGKSFTLTITV
T206 SGRGKSFTLTITVFT
T208 RGKSFTLTITVFTNP
S245 HRQKLDDSKPSLFSD
S248 KLDDSKPSLFSDRLS
S255 SLFSDRLSDLGRIPH
R275 GVPPQNPRPSLNSAP
S283 PSLNSAPSPFNPQGQ
S302 DPRQTQSSPPWSYDQ
S310 PPWSYDQSYPSYLSQ
S320 SYLSQMTSPSIHSTT
S322 LSQMTSPSIHSTTPL
T327 SPSIHSTTPLSSTRG
S348 TDVPRRISDDDTATS
T352 RRISDDDTATSDFCL
T354 ISDDDTATSDFCLWP
S355 SDDDTATSDFCLWPS
S373 KKSQAGASELGPFSD
S396 SLTESRFSNPRMHYP
R399 ESRFSNPRMHYPATF
S473 MVPGGDRSPSRMVPP
S507 DGVDADGSHSSSPTV
S511 ADGSHSSSPTVLNSS
Y529 DESVWRPY_______
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