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LC3A (human)

Overview LC3A is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Ubiquitin-like modifier; Autophagy; Vesicle protein; Microtubule binding protein Cellular Component: microtubule; late endosome; endomembrane system; autophagic vacuole; cytoplasmic vesicle; cytosol Molecular Function: protein binding; phosphatidylethanolamine binding; phospholipid binding Biological Process: autophagic vacuole formation Reference #:  Q9H492 (UniProtKB) Alt. Names/Synonyms: Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; MAP1LC3A; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A Gene Symbols: MAP1LC3A Molecular weight: 14,272 Da Basal Isoelectric point: 8.73  Predict pI for various phosphorylation states CST Pathways:  Autophagy Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below LC3A 3ECI (human)

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	Sites Implicated In
autophagy, altered: S12‑p intracellular localization: S12‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
1	0		S12-p	RPFKQRRsFADRCKE
0	1		K42-u	IERYKGEkQLPVLDK
0	1		K51	LPVLDKTKFLVPDHV
0	1		S92-p	QHSMVSVsTPIADIY

	mouse
S12-p	RPFKQRRsFADRCKE
K42	IERYKGEKQLPVLDK
K51-u	LPVLDKTkFLVPDHV
S92	QHSMVSVSTPIADIY

	rat
S12-p	RPFKQRRsFADRCKE
K42	IERYKGEKQLPVLDK
K51	LPVLDKTKFLVPDHV
S92	QHSMVSVSTPIADIY

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