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Protein Page:
LC3A (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LC3A is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Vesicle protein; Autophagy; Ubiquitin-like modifier; Microtubule binding protein
Cellular Component: microtubule; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol
Molecular Function: protein binding; phosphatidylethanolamine binding; phospholipid binding
Biological Process: mitochondrion degradation; autophagic vacuole formation
Reference #:  Q9H492 (UniProtKB)
Alt. Names/Synonyms: Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; MAP1LC3; MAP1LC3A; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A
Gene Symbols: MAP1LC3A
Molecular weight: 14,272 Da
Basal Isoelectric point: 8.73  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

LC3A

Protein Structure Not Found.


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Sites Implicated In
autophagy, altered: S12‑p
intracellular localization: S12‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 S12-p RPFKQRRsFADRCKE
0 1 K42-ub IERYKGEkQLPVLDK
0 1 K51 LPVLDKTKFLVPDHV
0 1 S92-p QHSMVSVsTPIADIY
  mouse

 
S12-p RPFKQRRsFADRCKE
K42 IERYKGEKQLPVLDK
K51-ub LPVLDKTkFLVPDHV
S92 QHSMVSVSTPIADIY
  rat

 
S12-p RPFKQRRsFADRCKE
K42 IERYKGEKQLPVLDK
K51 LPVLDKTKFLVPDHV
S92 QHSMVSVSTPIADIY
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