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LC3B (human)

Overview LC3B is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome. Note: This description may include information from UniProtKB. Protein type: Microtubule binding protein; Ubiquitin-like modifier; Vesicle protein; Autophagy Cellular Component: microtubule; microtubule associated complex; cell soma; axon; dendrite; endomembrane system; autophagic vacuole; intracellular; cytoplasmic vesicle Molecular Function: protein domain specific binding; protein binding; microtubule binding Biological Process: positive regulation of protein binding; autophagy Reference #:  Q9GZQ8 (UniProtKB) Alt. Names/Synonyms: Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B Gene Symbols: MAP1LC3B Molecular weight: 14,688 Da Basal Isoelectric point: 8.89  Predict pI for various phosphorylation states CST Pathways:  Autophagy Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below LC3B 1V49 (human) 2ZJD (human) 1UGM (rat) 2K6Q (rat) 2Z0D (rat) 2Z0E (rat) 2ZZP (rat)

STRING  |  Scansite  |  Phospho.ELM  |  Pfam  |  RCSB PDB 1V49 2ZJD  |  Phospho3D  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
1	1		T6-p	__MPSEKtFKQRRTF
1	0		T29-p	LIREQHPtKIPVIIE
0	1		K42-u	IERYKGEkQLPVLDK
0	2		K51-u	LPVLDKTkFLVPDHV
0	4		K65-u	VNMSELIkIIRRRLQ

	mouse
T6	__MPSEKTFKQRRSF
T29	LIREQHPTKIPVIIE
K42	IERYKGEKQLPVLDK
K51-u	LPVLDKTkFLVPDHV
K65-u	VNMSELIkIIRRRLQ

	rat
T6	__MPSEKTFKQRRSF
T29	LIREQHPTKIPVIIE
K42	IERYKGEKQLPVLDK
K51	LPVLDKTKFLVPDHV
K65	VNMSELIKIIRRRLQ

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