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Protein Page:
LC3B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LC3B is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin-like modifier; Vesicle protein; Autophagy; Microtubule binding protein
Chromosomal Location of Human Ortholog: 16q24.2
Cellular Component: microtubule; endomembrane system; autophagic vacuole; axoneme; intracellular; cytoplasmic vesicle
Molecular Function: protein binding
Biological Process: autophagy
Reference #:  Q9GZQ8 (UniProtKB)
Alt. Names/Synonyms: Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B
Gene Symbols: MAP1LC3B
Molecular weight: 14,688 Da
Basal Isoelectric point: 8.89  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

LC3B

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 1 T6-p __MPSEKtFKQRRTF
1 0 T29-p LIREQHPtKIPVIIE
0 1 K42-ub IERYKGEkQLPVLDK
0 2 K51-ub LPVLDKTkFLVPDHV
0 4 K65-ub VNMSELIkIIRRRLQ
  mouse

 
T6 __MPSEKTFKQRRSF
T29 LIREQHPTKIPVIIE
K42 IERYKGEKQLPVLDK
K51-ub LPVLDKTkFLVPDHV
K65-ub VNMSELIkIIRRRLQ
  rat

 
T6 __MPSEKTFKQRRSF
T29 LIREQHPTKIPVIIE
K42 IERYKGEKQLPVLDK
K51 LPVLDKTKFLVPDHV
K65 VNMSELIKIIRRRLQ
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