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WRN
Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily. Note: This description may include information from UniProtKB.
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| Protein type: EC 3.1.-.-; EC 3.6.1.-; DNA binding protein; EC 3.6.4.12; DNA repair; Cell cycle regulation; Helicase |
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Cellular Component: nucleoplasm; MutLalpha complex; centrosome; nucleolus; nucleus
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Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; helicase activity; Y-form DNA binding; ATP-dependent DNA helicase activity; four-way junction helicase activity; DNA helicase activity; protein binding; DNA binding; ATP-dependent 3'-5' DNA helicase activity; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding
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Biological Process: ATP catabolic process; nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; cell aging; multicellular organismal aging; cellular response to starvation; replicative cell aging; response to UV-C; DNA duplex unwinding; replication fork processing; DNA recombination; regulation of apoptosis; DNA synthesis during DNA repair; base-excision repair; regulation of growth rate; response to oxidative stress; DNA replication; DNA metabolic process; response to DNA damage stimulus; telomere maintenance; aging
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Reference #:
Q14191 (UniProtKB)
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| Alt. Names/Synonyms: DKFZp686C2056; DNA helicase, RecQ-like type 3; RecQ protein-like 2; RECQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome, RecQ helicase-like; WRN |
| Gene Symbols: WRN |
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Molecular weight: 162,461 Da
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Basal Isoelectric point: 5.96
Predict pI for various phosphorylation states
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