Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily. Note: This description may include information from UniProtKB.
Protein type: Nucleolus; Helicase; DNA repair, damage; EC 126.96.36.199; DNA-binding; Cell cycle regulation
Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; 3'-5' DNA helicase activity; magnesium ion binding; bubble DNA binding; helicase activity; four-way junction helicase activity; Y-form DNA binding; ATP-dependent DNA helicase activity; DNA helicase activity; protein binding; ATP-dependent 3'-5' DNA helicase activity; DNA binding; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding
Biological Process: nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; multicellular organismal aging; cell aging; post-translational protein modification; cellular response to starvation; replicative cell aging; response to UV-C; DNA duplex unwinding; DNA recombination; replication fork processing; regulation of apoptosis; DNA synthesis during DNA repair; protein sumoylation; cellular protein metabolic process; base-excision repair; double-strand break repair; regulation of growth rate; response to oxidative stress; DNA replication; telomere maintenance; DNA metabolic process; response to DNA damage stimulus; aging
LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.