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Protein Page:
Mad1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Mad1 Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with RNF17. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription, coactivator/corepressor
Cellular Component: nucleus
Molecular Function: protein dimerization activity; transcription cofactor activity; transcription factor activity; transcription corepressor activity
Biological Process: cell proliferation; transcription, DNA-dependent; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter
Reference #:  Q05195 (UniProtKB)
Alt. Names/Synonyms: MAD; MAD protein (MAX-binding protein); MAD1; Max dimerization protein 1; Max dimerizer 1; MGC104659; MXD1; Protein MAD
Gene Symbols: MXD1
Molecular weight: 25,254 Da
Basal Isoelectric point: 8.68  Predict pI for various phosphorylation states
CST Pathways:  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Mad1

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: S145‑p
cell growth, altered: S145‑p
transcription, induced: S145‑p
molecular association, regulation: S145‑p
protein degradation: S145‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K39-ac SMLPYNNkDRDALKR
0 6 S89-p LVPLGPEssRHTTLS
0 6 S90-p VPLGPEssRHTTLSL
2 0 S145-p IERIRMDsIGSTVSS
0 1 S191-p SDSDERGsMQsLGSD
0 1 S194-p DERGsMQsLGSDEGy
0 1 Y201-p sLGSDEGySSTSIKR
  mouse

 
K38 ASMLPYSKDRDAFKR
S88-p LVPLGPEssRHTTLS
S89-p VPLGPEssRHTTLSL
S144 AERTRMDSVGSVVSS
S197 SDSDERGSMQSLGSD
S200 DERGSMQSLGSDEGY
Y207 SLGSDEGYSSATVKR
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