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Protein Page:
HIF1AN (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HIF1AN Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4. Note: This description may include information from UniProtKB.
Protein type: EC 1.14.11.n4; EC 1.14.11.30; Oxidoreductase
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleolus; cytosol; nucleus
Molecular Function: NF-kappaB binding; protein binding; protein homodimerization activity; oxygen sensor activity; zinc ion binding; carboxylic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; cofactor binding; Notch binding
Biological Process: peptidyl-aspartic acid hydroxylation; transcription, DNA-dependent; peptidyl-asparagine hydroxylation; negative regulation of Notch signaling pathway; positive regulation of myoblast differentiation
Reference #:  Q9NWT6 (UniProtKB)
Alt. Names/Synonyms: DKFZp762F1811; Factor inhibiting HIF-1; factor inhibiting HIF1; FIH-1; FIH1; FLJ20615; FLJ22027; HIF1AN; HIF1N; hypoxia inducible factor 1, alpha subunit inhibitor; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
Gene Symbols: HIF1AN
Molecular weight: 40,285 Da
Basal Isoelectric point: 5.39  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HIF1AN

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y102-p ASTHKFLyYDEKKMA
0 2 K115-ub MANFQNFkPRSNREE
0 2 K131-ub KFHEFVEkLQDIQQR
0 1 K345-ub PLLNTMIkGRYN___
  mouse

 
Y102 ASTHKFLYYDEKKMG
K115 MGNFQNFKPRSNREE
K131 KFHEFVEKLQAIQQR
K345 PLLNTMIKGRYN___
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