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Protein Page:
POLR3B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
POLR3B DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Defects in POLR3B are the cause of leukodystrophy, hypomyelinating, type 8, with or without oligodontia and/or hypogonadotropic hypogonadism (HLD8). An autosomal recessive neurodegenerative disorder characterized by early childhood onset of cerebellar ataxia and mild intellectual disabilities associated with diffuse hypomyelination apparent on brain MRI. Variable features include oligodontia and/or hypogonadotropic hypogonadism. Belongs to the RNA polymerase beta chain family. Note: This description may include information from UniProtKB.
Protein type: Nucleotide Metabolism - purine; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Transferase; Transcription initiation complex
Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol
Molecular Function: DNA binding; ribonucleoside binding; DNA-directed RNA polymerase activity; metal ion binding
Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter
Reference #:  Q9NW08 (UniProtKB)
Alt. Names/Synonyms: C128; DKFZp686B10117; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B; DNA-directed RNA polymerase III subunit RPC2; FLJ10388; POLR3B; polymerase (RNA) III (DNA directed) polypeptide B; RNA polymerase III subunit C2; RPC2
Gene Symbols: POLR3B
Molecular weight: 127,785 Da
Basal Isoelectric point: 8.77  Predict pI for various phosphorylation states
Select Structure to View Below

POLR3B

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T12-p AEEFGNLtPEQLAAP
0 2 S202-p RKGAVGAsVTSsTHE
0 1 S206-p VGAsVTSsTHEkKSR
0 1 K210-ub VTSsTHEkKSRTNMA
0 1 T304-p WGGGPKKtKIEEARE
0 1 K324-ub ILTHVPVkEFNFRAK
0 1 S680-p PYPHHNQsPRNTYQC
0 1 K691 TYQCAMGKQAMGTIG
0 1 K723-ub QKPMVKTkTIELIEF
0 1 S816-p LDADGICsPGEKVEN
0 1 K852-ub VPQQPQYkDVPITYK
0 1 S872-p YIEKVMIssNAEDAF
0 1 S873-p IEKVMIssNAEDAFL
0 2 K950-ub LIELLAGkAGVLDGR
0 6 K1013-m1 PVYYQKLkHMVLDkM
0 1 K1019-ub LkHMVLDkMHARARG
0 1 T1036-p AVLTRQPtEGRSRDG
  mouse

 
T12 AEEFGSLTPEQLTAP
S202 RKGAVGASVTSSTHE
S206 VGASVTSSTHEKKSR
K210 VTSSTHEKKSRTNMA
T304 WGGGPKKTKIEEARE
K324 ILTHVPVKEFNFRAK
S680 PYPHHNQSPRNTYQC
K691-ub TYQCAMGkQAMGTIG
K723 QKPMVKTKTIELIDF
S816 LDADGICSPGEKVEN
K852 VPQQPQYKDVPITYK
S872 YIEKVMISSNAEDAF
S873 IEKVMISSNAEDAFL
K950-ub LIELLAGkAGVLDGR
K1013 PVYYQKLKHMVLDKM
K1019 LKHMVLDKMHARARG
T1036 AVLTRQPTEGRSRDG
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