Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family. Note: This description may include information from UniProtKB.
Protein type: EC 220.127.116.11; EC 18.104.22.168; Cofactor and Vitamin Metabolism - retinol; Membrane protein, integral; Cell surface; Oxidoreductase; EC 22.214.171.124; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.13.-; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 126.96.36.199
Chromosomal Location of Human Ortholog: 7q21.1
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm; integral to membrane
Molecular Function: quinine 3-monooxygenase activity; taurochenodeoxycholate 6alpha-hydroxylase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; albendazole monooxygenase activity; oxidoreductase activity; oxygen binding; steroid binding; testosterone 6-beta-hydroxylase activity; vitamin D3 25-hydroxylase activity; enzyme binding; iron ion binding; heme binding; steroid hydroxylase activity; monooxygenase activity
Biological Process: steroid metabolic process; drug catabolic process; xenobiotic metabolic process; androgen metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; alkaloid catabolic process; lipid metabolic process; heterocycle metabolic process; drug metabolic process; steroid catabolic process; vitamin D metabolic process
SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.