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Protein Page:
EphA2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
EphA2 a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; Eph family
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: focal adhesion; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding
Biological Process: neural tube development; axial mesoderm formation; cell migration; negative regulation of protein kinase B signaling cascade; peptidyl-tyrosine phosphorylation; viral reproduction; activation of Rac GTPase; multicellular organismal development; notochord formation; mammary gland epithelial cell proliferation; osteoclast differentiation; bone remodeling; regulation of blood vessel endothelial cell migration; regulation of angiogenesis; neuron differentiation; keratinocyte differentiation; osteoblast differentiation; regulation of cell adhesion mediated by integrin; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development; protein kinase B signaling cascade; ephrin receptor signaling pathway; angiogenesis; cell adhesion; vasculogenesis; skeletal development
Reference #:  P29317 (UniProtKB)
Alt. Names/Synonyms: ARCC2; ECK; EPH receptor A2; EPHA2; ephrin receptor EphA2; Ephrin type-A receptor 2; Epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; protein tyrosine kinase; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK
Gene Symbols: EPHA2
Molecular weight: 108,266 Da
Basal Isoelectric point: 5.86  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

EphA2

Protein Structure Not Found.


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Sites Implicated In
carcinogenesis, induced: S897‑p
cell adhesion, altered: Y588‑p
cell differentiation, altered: Y588‑p, Y594‑p, Y735‑p, Y930‑p
cell motility, altered: Y588‑p, Y594‑p, Y735‑p, S897‑p
cell motility, induced: Y930‑p
enzymatic activity, induced: Y588‑p, Y594‑p, Y930‑p
intracellular localization: S897‑p
molecular association, regulation: Y588‑p, Y594‑p, Y735‑p, Y921‑p, Y930‑p, Y960‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y67-p NDMPIYMySVCNVMS
0 1 S113 NSFPGGASSCKETFN
0 1 S114 SFPGGASSCKETFNL
0 1 K136-ub DYGTNFQkRLFTkID
0 1 K141-ub FQkRLFTkIDTIAPD
0 1 S153-p APDEITVsSDFEARH
0 1 K162-ub DFEARHVkLNVEERS
0 1 S373-p CEQCWPEsGECGPCE
0 1 S426-p SGLVTSRsFRTASVs
0 1 S433-p sFRTASVsINQtEPP
0 1 T437-p ASVsINQtEPPKVRL
0 11 S570-p KNQRARQsPEDVyFs
0 329 Y575-p RQsPEDVyFsksEQL
0 2 S577-p sPEDVyFsksEQLkP
0 4 K578-ub PEDVyFsksEQLkPL
0 5 S579-p EDVyFsksEQLkPLk
0 6 K583-ub FsksEQLkPLktyVD
0 1 K586-ub sEQLkPLktyVDPHt
0 78 T587-p EQLkPLktyVDPHty
2 653 Y588-p QLkPLktyVDPHtyE
0 149 T593-p ktyVDPHtyEDPNQA
2 748 Y594-p tyVDPHtyEDPNQAV
0 1 K603 DPNQAVLKFTTEIHP
0 4 K617-ub PSCVTRQkVIGAGEF
0 48 Y628-p AGEFGEVyKGMLKTS
0 1 K639-ub LKTSSGKkEVPVAIK
0 3 T647-p EVPVAIKtLKAGYTE
0 1 K655-ub LKAGYTEkQRVDFLG
0 2 Y685-p LEGVISKykPMMIIt
0 2 K686-ub EGVISKykPMMIItE
0 2 T692-p ykPMMIItEyMENGA
0 18 Y694-p PMMIItEyMENGALD
0 1 S712-p REKDGEFsVLQLVGM
1 15 Y735-p KYLANMNyVHRDLAA
0 3 S761-p KVSDFGLsRVLEDDP
0 30 T771-p LEDDPEAtyttsGGk
2 873 Y772-p EDDPEAtyttsGGkI
0 24 T773-p DDPEAtyttsGGkIP
0 18 T774-p DPEAtyttsGGkIPI
0 2 S775-p PEAtyttsGGkIPIR
0 5 K778-ub tyttsGGkIPIRWTA
0 1 S790-p WTAPEAIsyRKFTSA
0 8 Y791-p TAPEAIsyRKFTSAS
0 1 S869-p PKFADIVsILDKLIR
0 1 K873 DIVsILDKLIRAPDs
0 1 S880-p KLIRAPDsLkTLADF
0 2 K882-ub IRAPDsLkTLADFDP
0 58 S892-p ADFDPRVsIRLPsts
1 181 S897-p RVsIRLPstsGsEGV
0 58 T898-p VsIRLPstsGsEGVP
0 83 S899-p sIRLPstsGsEGVPF
0 109 S901-p RLPstsGsEGVPFRT
0 2 S910-p GVPFRTVsEWLESIK
1 31 Y921-p ESIKMQQytEHFMAA
0 15 T922-p SIKMQQytEHFMAAG
3 16 Y930-p EHFMAAGyTAIEKVV
0 3 T940-p IEKVVQMtNDDIkRI
0 1 K945-ub QMtNDDIkRIGVRLP
1 60 Y960-p GHQKRIAysLLGLKD
0 1 S961-p HQKRIAysLLGLKDQ
7423 : Phospho-EphA2 (Tyr588) Antibody
12677 : Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb
3970 : Phospho-EphA2 (Tyr594) Antibody
8244 : Phospho-EphA2 (Tyr772) Antibody
8862 : Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb
6347 : Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb
  mouse

 
Y66 DDMPIYMYSVCNVVS
S112-p NSFPGGAssCKETFN
S113-p SFPGGAssCKETFNL
K135 DYGTNFQKRQFTKID
K140 FQKRQFTKIDTIAPD
S152 APDEITVSSDFEARN
K161 DFEARNVKLNVEERM
S374 CEQCWPESGECGPCE
S427 SGLVTSRSFRTASVS
S434 SFRTASVSINQTEPP
T438 ASVSINQTEPPKVRL
S571-p RNLRARQsSEDVRFS
R576 RQsSEDVRFSksEQL
S578 sSEDVRFSksEQLkP
K579-ub SEDVRFSksEQLkPL
S580-p EDVRFSksEQLkPLK
K584-ub FSksEQLkPLKtyVD
K587 sEQLkPLKtyVDPHt
T588-p EQLkPLKtyVDPHty
Y589-p QLkPLKtyVDPHtyE
T594-p KtyVDPHtyEDPNQA
Y595-p tyVDPHtyEDPNQAV
K604-ub DPNQAVLkFTTEIHP
K618-ub PSCVARQkVIGAGEF
Y629-p AGEFGEVyKGTLKAS
K640 LKASSGKKEIPVAIK
T648 EIPVAIKTLKAGYTE
K656 LKAGYTEKQRVDFLS
Y686 LEGVVSKYKPMMIIT
K687 EGVVSKYKPMMIITE
T693 YKPMMIITEYMENGA
Y695 PMMIITEYMENGALD
S713 REKDGEFSVLQLVGM
Y736-p KYLANMNyVHRDLAA
S762-p KVSDFGLsRVLEDDP
T772-p LEDDPEAtyTtsGGK
Y773-p EDDPEAtyTtsGGKI
T774 DDPEAtyTtsGGKIP
T775-p DPEAtyTtsGGKIPI
S776-p PEAtyTtsGGKIPIR
K779 tyTtsGGKIPIRWTA
S791 WTAPEAISYRKFTSA
Y792 TAPEAISYRKFTSAS
S870 PKFADIVSILDkLIR
K874-ub DIVSILDkLIRAPDS
S881 kLIRAPDSLkTLADF
K883-ub IRAPDSLkTLADFDP
S893-p ADFDPRVsIRLPsts
S898-p RVsIRLPstsGsEGV
T899-p VsIRLPstsGsEGVP
S900-p sIRLPstsGsEGVPF
S902-p RLPstsGsEGVPFRT
S911 GVPFRTVSEWLESIK
Y922-p ESIKMQQyTEHFMVA
T923 SIKMQQyTEHFMVAG
Y931 EHFMVAGYTAIEKVV
S941 IEKVVQMSNEDIKRI
K946 QMSNEDIKRIGVRLP
Y961-p GHQKRIAySLLGLKD
S962 HQKRIAySLLGLKDQ
7423 : Phospho-EphA2 (Tyr588) Antibody
12677 : Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb
3970 : Phospho-EphA2 (Tyr594) Antibody
8244 : Phospho-EphA2 (Tyr772) Antibody
8862 : Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb
6347 : Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb
  rat

 
Y66 NDMPIYMYSVCNVVS
S112 NSFPGGASSCKETFN
S113 SFPGGASSCKETFNL
K135 DYGTNFQKRQFTKID
K140 FQKRQFTKIDTIAPD
S152 APDEITVSSDFEARN
K161 DFEARNVKLNVEERM
S374 CEQCWPESGECGSCE
S427 SDLVNSRSFRTASVS
S434 SFRTASVSINQTEPP
T438 ASVSINQTEPPKVRL
S571-p RNLRARQsSEDVYFS
Y576 RQsSEDVYFSKSEQL
S578 sSEDVYFSKSEQLKP
K579 SEDVYFSKSEQLKPL
S580 EDVYFSKSEQLKPLK
K584 FSKSEQLKPLKTYVD
K587 SEQLKPLKTYVDPHT
T588 EQLKPLKTYVDPHTY
Y589 QLKPLKTYVDPHTYE
T594 KTYVDPHTYEDPNQA
Y595 TYVDPHTYEDPNQAV
K604 DPNQAVLKFTTEIHP
K618 PSCVSRQKVIGAGEF
Y629 AGEFGEVYKGTLKSS
K640 LKSSSGKKEIPVAIK
T648 EIPVAIKTLKAGYTE
K656 LKAGYTEKQRVDFLS
Y686 LEGVVSKYKPMMIIT
K687 EGVVSKYKPMMIITE
T693 YKPMMIITEYMENGA
Y695 PMMIITEYMENGALD
S713 REKDGEFSVLQLVGM
Y736 KYLANMNYVHRDLAA
S762 KVSDFGLSRVLEDDP
T772 LEDDPEATyTTSGGK
Y773-p EDDPEATyTTSGGKI
T774 DDPEATyTTSGGKIP
T775 DPEATyTTSGGKIPI
S776 PEATyTTSGGKIPIR
K779 TyTTSGGKIPIRWTA
S791 WTAPEAISYRKFTSA
Y792 TAPEAISYRKFTSAS
S870 PKFADIVSILDKLIR
K874 DIVSILDKLIRAPDS
S881 KLIRAPDSLKTLADF
K883 IRAPDSLKTLADFDP
S893 ADFDPRVSIRLPSTS
S898 RVSIRLPSTSGSEGV
T899 VSIRLPSTSGSEGVP
S900 SIRLPSTSGSEGVPF
S902 RLPSTSGSEGVPFRT
S911 GVPFRTVSEWLESIK
Y922 ESIKMQQYTEHFMVA
T923 SIKMQQYTEHFMVAG
Y931 EHFMVAGYTAIEKVV
S941 IEKVVQMSNDDIKRI
K946 QMSNDDIKRIGVRLP
Y961 GHQKRIAYSLLGLKD
S962 HQKRIAYSLLGLKDQ
7423 : Phospho-EphA2 (Tyr588) Antibody
12677 : Phospho-EphA2 (Tyr588) (D7X2L) Rabbit mAb
3970 : Phospho-EphA2 (Tyr594) Antibody
8244 : Phospho-EphA2 (Tyr772) Antibody
8862 : Phospho-EphA3 (Tyr779) (D10H1) Rabbit mAb
6347 : Phospho-EphA2 (Ser897) (D9A1) Rabbit mAb
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