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Protein Page:
HIRA (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HIRA a histone chaperone. Necessary to mediate DNA-synthesis-independent nucleosome assembly. Interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner. Required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation. Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive. While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence. HIRA is ubiquitously expressed during mouse embryonic development. In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver. A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development. Two isoforms of the human protein result from alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cell development/differentiation; Cell cycle regulation; Transcription regulation
Cellular Component: nucleoplasm; PML body; protein complex; nuclear chromatin; nucleus
Molecular Function: protein binding; chromatin binding; transcription corepressor activity; transcription factor activity
Biological Process: osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; anatomical structure morphogenesis; DNA replication-independent nucleosome assembly; transcription, DNA-dependent; gastrulation; chromatin modification
Reference #:  P54198 (UniProtKB)
Alt. Names/Synonyms: DGCR1; DiGeorge critical region gene 1; HIR; HIR histone cell cycle regulation defective homolog A (S. cerevisiae); HIRA; Protein HIRA; TUP1; TUP1-like enhancer of split protein 1; TUPLE1
Gene Symbols: HIRA
Molecular weight: 111,835 Da
Basal Isoelectric point: 8.4  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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HIRA

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T7 _MKLLKPTWVNHNGK
0 1 K27-ub DIHPDGTkFATGGQG
0 1 S111-p GPSTVFGsSGkLANV
0 3 K114-ac TVFGsSGkLANVEQW
0 1 K114-ub TVFGsSGkLANVEQW
0 1 K178-ub RGHSGLVkGLTWDPV
0 1 K187-ub LTWDPVGkYIASQAD
0 1 T393-p MTEAQLStAVIENPE
0 3 Y404-p ENPEMLKyQRRQQQQ
0 1 T544-p DSMNATStPAALsPS
0 5 S549-p TStPAALsPSVLttP
0 2 T554-p ALsPSVLttPsKIEP
1 5 T555-p LsPSVLttPsKIEPM
0 1 S557-p PSVLttPsKIEPMKA
0 1 K563 PsKIEPMKAFDSRFT
0 2 S573-p DSRFTERsKAtPGAP
0 3 T576-p FTERsKAtPGAPALt
0 1 A581 KAtPGAPALtsMtPt
1 2 T583-p tPGAPALtsMtPtAV
1 6 S584-p PGAPALtsMtPtAVE
1 7 T586-p APALtsMtPtAVERL
1 1 T588-p ALtsMtPtAVERLKE
0 5 S610-p RPRDLLEsssDsDEK
0 5 S611-p PRDLLEsssDsDEKV
0 6 S612-p RDLLEsssDsDEKVP
0 5 S614-p LLEsssDsDEKVPLA
0 1 T637-p KLELEVEtVEKKKKG
0 29 S650-p KGRPRKDsRLMPVsL
0 1 S656-p DsRLMPVsLsVQsPA
0 1 S658-p RLMPVsLsVQsPAAL
0 63 S661-p PVsLsVQsPAALTAE
0 1 S675-p EKEAMCLsAPALALK
0 3 S687-p ALKLPIPsPQRAFTL
0 1 S697-p RAFTLQVsSDPSMYI
0 1 T711-p IEVENEVtVVGGVKL
0 1 S812-p VVVVKEEsLHsILAG
0 1 S815-p VKEEsLHsILAGSDM
0 1 Y945-p WLLVYARyLVNEGFE
0 1 Y953-p LVNEGFEyRLREICK
  mouse

 
T7-p _MKLLKPtWVNHNGK
K27 DIHPDGTKFATGGQG
S111 GPSTVFGSSGKLANV
K114 TVFGSSGKLANVEQW
K114 TVFGSSGKLANVEQW
K178 RGHSGLVKGLTWDPV
K187 LTWDPVGKYIASQAD
T393 MTEAQLSTAVIENPE
Y404 ENPEMLKYQRRQQQQ
T543 DSMNATSTPAASSPS
S548 TSTPAASSPSVLTTP
T553 ASSPSVLTTPSKIEP
T554 SSPSVLTTPSKIEPM
S556 PSVLTTPSKIEPMKA
K562 PSKIEPMKAFDSRFT
S572-p DSRFTERsKAtPGAP
T575-p FTERsKAtPGAPsLt
S580-p KAtPGAPsLtSVIPT
T582-p tPGAPsLtSVIPTAV
S583 PGAPsLtSVIPTAVE
I585 APsLtSVIPTAVERL
T587 sLtSVIPTAVERLKE
S608 LRSRELESSSDSDEK
S609 RSRELESSSDSDEKV
S610 SRELESSSDSDEKVH
S612 ELESSSDSDEKVHLA
T635 KLELEVETVEKKKKG
S648 KGRPRKDSRLLPMSL
S654 DSRLLPMSLSVQsPA
S656 RLLPMSLSVQsPAAL
S659-p PMSLSVQsPAALSTE
S673 EKEAMCLSAPALALK
G685 ALKLPIPGPQRAFTL
S695 RAFTLQVSSDPSMYI
T709 IEVENEVTTVGGIRL
S810 VVVVKEESLHSILSG
S813 VKEESLHSILSGSDM
Y943 WLLLYARYLVNEGFE
Y951 LVNEGFEYRLREICK
  rat

 
T7 _MKLLKPTWVNHNGK
K27 DIHPDGTKFATGGQG
S111 GPSTVFGSSGKLANV
K114 TVFGSSGKLANVEQW
K114 TVFGSSGKLANVEQW
K178 RGHSGLVKGLTWDPV
K187 LTWDPVGKYIASQAD
T393 MTEAQLSTAVIENPE
Y404 ENPEMLKYQRRQQQQ
T543 DSMNATSTPAALsPS
S548-p TSTPAALsPSVLTTP
T553 ALsPSVLTTPSKIEP
T554 LsPSVLTTPSKIEPM
S556 PSVLTTPSKIEPMkA
K562-ub PSKIEPMkAFDSRFT
S572 DSRFTERSKATPGAP
T575 FTERSKATPGAPSLT
S580 KATPGAPSLTSVIPT
T582 TPGAPSLTSVIPTAV
S583 PGAPSLTSVIPTAVE
I585 APSLTSVIPTAVERL
T587 SLTSVIPTAVERLKE
S608 LRSRELDSSSDSDEK
S609 RSRELDSSSDSDEKV
S610 SRELDSSSDSDEKVH
S612 ELDSSSDSDEKVHLA
T635 KLELEVETVEKKKKG
S648 KGRPRKDSRLLPVSL
S654 DSRLLPVSLSVQsPA
S656 RLLPVSLSVQsPAAL
S659-p PVSLSVQsPAALSAE
S673 EKEAMCLSAPALALK
G685 ALKLPIPGPQRAFTL
S695 RAFTLQVSSDPSMYI
T709 IEVENEVTTVGGIRL
S810 VVVVKEESLHSILSG
S813 VKEESLHSILSGSDM
Y943 WLLLYARYLVNEGFE
Y951 LVNEGFEYRLREICQ
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