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Protein Page:
EWS (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
EWS an oncogenic RNA-binding protein. Might normally function as a repressor. Binds RPB3, SF1, calmodulin and RNA. Interacts with and regulated by Pyk2. Relocates from cytoplasm to ribosomes upon Pyk2 activation. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. 5 alternatively-spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: RNA binding protein; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nucleus
Molecular Function: calmodulin binding; identical protein binding; protein binding; zinc ion binding; RNA binding; nucleotide binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Disease: Ewing Sarcoma
Reference #:  Q01844 (UniProtKB)
Alt. Names/Synonyms: bK984G1.4; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS-Fli1 (type 1) oncogene; EWS; EWS oncogene; EWSR1; RNA-binding protein EWS
Gene Symbols: EWSR1
Molecular weight: 68,478 Da
Basal Isoelectric point: 9.37  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

EWS

Protein Structure Not Found.


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Sites Implicated In
transcription, induced: S266‑p
intracellular localization: S266‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 T79-p QPPTGYTtPTAPQAY
2 0 S266-p SSYGQQSsFRQDHPS
1 4 Y278-p HPSSMGVyGQESGGF
1 0 R300-m1 SMSGPDNrGrGrGGF
2 0 R300-m2 SMSGPDNrGrGrGGF
2 0 R302-m2 SGPDNrGrGrGGFDr
0 1 R304-m1 PDNrGrGrGGFDrGG
2 0 R304-m2 PDNrGrGrGGFDrGG
1 1 R309-m1 rGrGGFDrGGMSrGG
2 0 R309-m2 rGrGGFDrGGMSrGG
2 1 R314-m2 FDrGGMSrGGrGGGr
2 1 R317-m2 GGMSrGGrGGGrGGM
2 1 R321-m2 rGGrGGGrGGMGSAG
0 1 - gap
1 0 R330-m1 GMGSAGErGGFNKPG
1 0 R330-m2 GMGSAGErGGFNKPG
0 1 S371-p YVQGLNDsVTLDDLA
0 13 Y417-p KGDATVSyEDPPTAk
0 41 K424-ub yEDPPTAkAAVEWFD
0 2 K433-ac AVEWFDGkDFQGSkL
0 8 K433-ub AVEWFDGkDFQGSkL
0 3 K439-ac GkDFQGSkLKVSLAR
0 2 K439-ub GkDFQGSkLKVSLAR
0 1 K441 DFQGSkLKVSLARKK
1 1 R455-m2 KPPMNSMrGGLPPrE
0 1 R455-m1 KPPMNSMrGGLPPrE
0 1 R461-m1 MrGGLPPrEGrGMPP
0 1 R464-m1 GLPPrEGrGMPPPLr
1 0 R464-m2 GLPPrEGrGMPPPLr
0 29 R471-m1 rGMPPPLrGGPGGPG
1 4 R471-m2 rGMPPPLrGGPGGPG
0 5 R486-m1 GPGGPMGrMGGrGGD
1 0 R490-m2 PMGrMGGrGGDrGGF
0 2 R494-m1 MGGrGGDrGGFPPrG
2 1 R494-m2 MGGrGGDrGGFPPrG
0 2 R500-m1 DrGGFPPrGPrGSrG
2 0 R500-m2 DrGGFPPrGPrGSrG
0 1 R503-m1 GFPPrGPrGSrGNPS
2 2 R503-m2 GFPPrGPrGSrGNPS
0 3 R506-m1 PrGPrGSrGNPSGGG
2 3 R506-m2 PrGPrGSrGNPSGGG
0 1 R563-m1 FPPPGGDrGrGGPGG
1 0 R563-m2 FPPPGGDrGrGGPGG
0 2 R565-m1 PPGGDrGrGGPGGMr
2 0 R565-m2 PPGGDrGrGGPGGMr
1 0 R572-m2 rGGPGGMrGGrGGLM
1 0 R575-m2 PGGMrGGrGGLMDrG
1 1 R581-m2 GrGGLMDrGGPGGMF
1 2 R589-m1 GGPGGMFrGGrGGDr
2 0 R589-m2 GGPGGMFrGGrGGDr
1 0 R592-m2 GGMFrGGrGGDrGGF
2 0 R596-m2 rGGrGGDrGGFrGGr
2 0 R600-m2 GGDrGGFrGGrGMDr
2 0 R603-m2 rGGFrGGrGMDrGGF
1 0 R607-m1 rGGrGMDrGGFGGGr
2 2 R607-m2 rGGrGMDrGGFGGGr
0 1 R614-m2 rGGFGGGrrGGPGGP
1 8 R615-m1 GGFGGGrrGGPGGPP
2 4 R615-m2 GGFGGGrrGGPGGPP
0 1 R632-m1 LMEQMGGrrGGrGGP
2 2 R633-m2 MEQMGGrrGGrGGPG
2 2 R636-m2 MGGrrGGrGGPGkMD
0 1 K641-ac GGrGGPGkMDKGEHR
0 1 K644 GGPGkMDKGEHRQER
1 0 Y656-p QERRDRPy_______
  EWS iso2  
T79 QPPTGYTTPTAPQAY
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S298 YVQGLNDSVTLDDLA
Y344 KGDATVSYEDPPTAK
K351 YEDPPTAKAAVEWFD
K360 AVEWFDGKDFQGSKL
K360 AVEWFDGKDFQGSKL
K366 GKDFQGSKLKVSLAR
K366 GKDFQGSKLKVSLAR
K368 DFQGSKLKVSLARKK
R382 KPPMNSMRGGLPPRE
R382 KPPMNSMRGGLPPRE
R388 MRGGLPPREGRGMPP
R391 GLPPREGRGMPPPLR
R391 GLPPREGRGMPPPLR
R398 RGMPPPLRGGPGGPG
R398 RGMPPPLRGGPGGPG
R413 GPGGPMGRMGGRGGD
R417 PMGRMGGRGGDRGGF
R421 MGGRGGDRGGFPPRG
R421 MGGRGGDRGGFPPRG
R427 DRGGFPPRGPRGSRG
R427 DRGGFPPRGPRGSRG
R430 GFPPRGPRGSRGNPS
R430 GFPPRGPRGSRGNPS
R433 PRGPRGSRGNPSGGG
R433 PRGPRGSRGNPSGGG
R490 FPPPGGDRGRGGPGG
R490 FPPPGGDRGRGGPGG
R492 PPGGDRGRGGPGGMR
R492 PPGGDRGRGGPGGMR
R499 RGGPGGMRGGRGGLM
R502 PGGMRGGRGGLMDRG
R508 GRGGLMDRGGPGGMF
R516 GGPGGMFRGGRGGDR
R516 GGPGGMFRGGRGGDR
R519 GGMFRGGRGGDRGGF
R523 RGGRGGDRGGFRGGR
R527 GGDRGGFRGGRGMDR
R530 RGGFRGGRGMDRGGF
R534 RGGRGMDRGGFGGGR
R534 RGGRGMDRGGFGGGR
R541 RGGFGGGRRGGPGGP
R542 GGFGGGRRGGPGGPP
R542 GGFGGGRRGGPGGPP
R559 LMEQMGGRRGGRGGP
R560 MEQMGGRRGGRGGPG
R563 MGGRRGGRGGPGKMD
K568 GGRGGPGKMDKGEHR
K571 GGPGKMDKGEHRQER
Y583 QERRDRPY_______
  EWS iso3  
T79 QPPTGYTTPTAPQAY
S266 SSYGQQSSFRQDHPS
Y278 HPSSMGVYGQESGGF
R300 SMSGPDNRGRGRGGF
R300 SMSGPDNRGRGRGGF
R302 SGPDNRGRGRGGFDR
R304 PDNRGRGRGGFDRGG
R304 PDNRGRGRGGFDRGG
R309 RGRGGFDRGGMSRGG
R309 RGRGGFDRGGMSRGG
R314 FDRGGMSRGGRGGGR
R317 GGMSRGGRGGGRGGM
R321 RGGRGGGRGGMGAGE
- gap
R329 GGMGAGERGGFNKPG
R329 GGMGAGERGGFNKPG
S370 YVQGLNDSVTLDDLA
Y416 KGDATVSYEDPPTAK
K423 YEDPPTAKAAVEWFD
K432 AVEWFDGKDFQGSKL
K432 AVEWFDGKDFQGSKL
K438 GKDFQGSKLKVSLAR
K438 GKDFQGSKLKVSLAR
K440 DFQGSKLKVSLARKK
R454 KPPMNSMRGGLPPRE
R454 KPPMNSMRGGLPPRE
R460 MRGGLPPREGRGMPP
R463 GLPPREGRGMPPPLR
R463 GLPPREGRGMPPPLR
R470 RGMPPPLRGGPGGPG
R470 RGMPPPLRGGPGGPG
R485 GPGGPMGRMGGRGGD
R489 PMGRMGGRGGDRGGF
R493 MGGRGGDRGGFPPRG
R493 MGGRGGDRGGFPPRG
R499 DRGGFPPRGPRGSRG
R499 DRGGFPPRGPRGSRG
R502 GFPPRGPRGSRGNPS
R502 GFPPRGPRGSRGNPS
R505 PRGPRGSRGNPSGGG
R505 PRGPRGSRGNPSGGG
R562 FPPPGGDRGRGGPGG
R562 FPPPGGDRGRGGPGG
R564 PPGGDRGRGGPGGMR
R564 PPGGDRGRGGPGGMR
R571 RGGPGGMRGGRGGLM
R574 PGGMRGGRGGLMDRG
R580 GRGGLMDRGGPGGMF
R588 GGPGGMFRGGRGGDR
R588 GGPGGMFRGGRGGDR
R591 GGMFRGGRGGDRGGF
R595 RGGRGGDRGGFRGGR
R599 GGDRGGFRGGRGMDR
R602 RGGFRGGRGMDRGGF
R606 RGGRGMDRGGFGGGR
R606 RGGRGMDRGGFGGGR
R613 RGGFGGGRRGGPGGP
R614 GGFGGGRRGGPGGPP
R614 GGFGGGRRGGPGGPP
R631 LMEQMGGRRGGRGGP
R632 MEQMGGRRGGRGGPG
R635 MGGRRGGRGGPGKMD
K640 GGRGGPGKMDKGEHR
K643 GGPGKMDKGEHRQER
Y655 QERRDRPY_______
  EWS iso4  
T79 QPPTGYTTPTAPQAY
S266 SSYGQQSSFRQDHPS
Y278 HPSSMGVYGQESGGF
R300 SMSGPDNRGRGRGGF
R300 SMSGPDNRGRGRGGF
R302 SGPDNRGRGRGGFDR
R304 PDNRGRGRGGFDRGG
R304 PDNRGRGRGGFDRGG
R309 RGRGGFDRGGMSRGG
R309 RGRGGFDRGGMSRGG
R314 FDRGGMSRGGRGGGR
R317 GGMSRGGRGGGRGGM
R321 RGGRGGGRGGMGLQs
S328-p RGGMGLQsESLVYTS
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- under review  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- under review  
- under review  
- gap
  mouse

 
T79-p QPPTGYStPTAPQAY
S266 SSYGQQSSFRQDHPS
Y278 HPSSMGVYGQESGGF
R300 SLSGPDNRGRGRGGF
R300 SLSGPDNRGRGRGGF
R302 SGPDNRGRGRGGFDR
R304 PDNRGRGRGGFDRGG
R304 PDNRGRGRGGFDRGG
R309 RGRGGFDRGGMSRGG
R309 RGRGGFDRGGMSRGG
R314 FDRGGMSRGGRGGGR
R317 GGMSRGGRGGGRGGL
R321 RGGRGGGRGGLGAGE
- gap
R329 GGLGAGERGGFNKPG
R329 GGLGAGERGGFNKPG
N370 YVQGLNDNVTLDDLA
Y416 KGDATVSYEDPPTAk
K423-ub YEDPPTAkAAVEWFD
K432 AVEWFDGKDFQGSKL
K432-ub AVEWFDGkDFQGSKL
K438 GkDFQGSKLKVSLAR
K438 GkDFQGSKLKVSLAR
K440 DFQGSKLKVSLARKK
R454 KPPMNSMRGGMPPRE
R454-m1 KPPMNSMrGGMPPRE
R460 MrGGMPPREGRGMPP
R463 GMPPREGRGMPPPLr
R463 GMPPREGRGMPPPLr
R470-m1 RGMPPPLrGGPGGPG
R470-m2 RGMPPPLrGGPGGPG
R485-m1 GPGGPMGrMGGRGGD
R489 PMGrMGGRGGDRGGF
R493 MGGRGGDRGGFPPRG
R493 MGGRGGDRGGFPPRG
R499 DRGGFPPRGPrGSrG
R499 DRGGFPPRGPrGSrG
R502 GFPPRGPRGSrGNPS
R502-m2 GFPPRGPrGSrGNPS
R505-m1 PRGPrGSrGNPSGGG
R505-m2 PRGPrGSrGNPSGGG
R562 FPPPGGDRGrGGPGG
R562 FPPPGGDRGrGGPGG
R564-m1 PPGGDRGrGGPGGMR
R564 PPGGDRGRGGPGGMR
R571 rGGPGGMRGGRGGLM
R574 PGGMRGGRGGLMDRG
R580 GRGGLMDRGGPGGMF
R588 GGPGGMFRGGRGGDR
R588 GGPGGMFRGGRGGDR
R591 GGMFRGGRGGDRGGF
R595 RGGRGGDRGGFRGGR
R599 GGDRGGFRGGRGMDr
R602 RGGFRGGRGMDrGGF
R606 RGGRGMDRGGFGGGR
R606-m2 RGGRGMDrGGFGGGR
R613 rGGFGGGRrGGPGGP
R614-m1 GGFGGGRrGGPGGPP
R614-m2 GGFGGGRrGGPGGPP
R631 LMEQMGGRrGGrGGP
R632-m2 MEQMGGRrGGrGGPG
R635-m2 MGGRrGGrGGPGKMD
K640 GGrGGPGKMDKGEHR
K643 GGPGKMDKGEHRQER
Y655 QERRDRPY_______
  rat

 
T79 QPPTGYTTPTAPQAY
S266 SSYGQQSSFRQDHPS
Y278 HPSSMGVYGQESGGF
R300 SLSGPDNRGRGRGGF
R300 SLSGPDNRGRGRGGF
R302 SGPDNRGRGRGGFDR
R304 PDNRGRGRGGFDRGG
R304 PDNRGRGRGGFDRGG
R309 RGRGGFDRGGMSRGG
R309 RGRGGFDRGGMSRGG
R314 FDRGGMSRGGRGGGR
R317 GGMSRGGRGGGRGGM
R321 RGGRGGGRGGMGAGE
- gap
R329 GGMGAGERGGFNKPG
R329 GGMGAGERGGFNKPG
S370 YVQGLNDSVTLDDLA
Y416 KGDATVSYEDPPTAK
K423 YEDPPTAKAAVEWFD
K432-ac AVEWFDGkDFQGSkL
K432 AVEWFDGKDFQGSkL
K438-ac GkDFQGSkLkVSLAR
K438 GkDFQGSKLkVSLAR
K440-ac DFQGSkLkVSLARKK
R454 KPPLNSMRGGMPPRE
R454 KPPLNSMRGGMPPRE
R460 MRGGMPPREGRGMPP
R463 GMPPREGRGMPPPLr
R463 GMPPREGRGMPPPLr
R470-m1 RGMPPPLrGGPGGPG
R470 RGMPPPLRGGPGGPG
R485-m1 GPGGPMGrMGGRGGD
R489 PMGrMGGRGGDRGGF
R493 MGGRGGDRGGFPPRG
R493 MGGRGGDRGGFPPRG
R499 DRGGFPPRGPRGSRG
R499 DRGGFPPRGPRGSRG
R502 GFPPRGPRGSRGNPS
R502 GFPPRGPRGSRGNPS
R505 PRGPRGSRGNPSGGG
R505 PRGPRGSRGNPSGGG
R562 FPPPGGDRGRGGPGG
R562 FPPPGGDRGRGGPGG
R564 PPGGDRGRGGPGGMR
R564 PPGGDRGRGGPGGMR
R571 RGGPGGMRGGRGGLM
R574 PGGMRGGRGGLMDRG
R580 GRGGLMDRGGPGGMF
R588 GGPGGMFRGGRGGDR
R588 GGPGGMFRGGRGGDR
R591 GGMFRGGRGGDRGGF
R595 RGGRGGDRGGFRGGR
R599 GGDRGGFRGGRGMDR
R602 RGGFRGGRGMDRGGF
R606 RGGRGMDRGGFGGGR
R606 RGGRGMDRGGFGGGR
R613 RGGFGGGRrGGPGGP
R614-m1 GGFGGGRrGGPGGPP
R614 GGFGGGRRGGPGGPP
R631 LMEQMGGRRGGRGGP
R632 MEQMGGRRGGRGGPG
R635 MGGRRGGRGGPGkMD
K640-ac GGRGGPGkMDkGEHR
K643-ac GGPGkMDkGEHRQER
Y655 QERRDRPY_______
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