required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation
Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; nucleus; cytosol
Molecular Function: protein binding; protein homodimerization activity
Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; mitotic cell cycle checkpoint; M phase of mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; negative regulation of protein catabolic process; cell cycle checkpoint; mitotic prometaphase; negative regulation of apoptosis
SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.