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MCF2 (human)

Overview MCF2 Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42. Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4- phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1. Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis. Belongs to the MCF2 family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Guanine nucleotide exchange factor, Rac/Rho; Cell development/differentiation; Motility/polarity/chemotaxis; Apoptosis Cellular Component: cytoskeleton; membrane; cytosol Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; phospholipid binding Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; apoptosis; small GTPase mediated signal transduction; regulation of Rho protein signal transduction Reference #:  P10911 (UniProtKB) Alt. Names/Synonyms: ARHGEF21; DBL; DBL-transforming protein; MCF.2 cell line derived transforming sequence; MCF2; MCF2-transforming protein; Oncogene MCF2 (oncogene DBL); Proto-oncogene DBL; Proto-oncogene MCF-2 Gene Symbols: MCF2 Molecular weight: 107,673 Da Basal Isoelectric point: 5.72  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below MCF2

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human ► Hide Isoforms
0	1		S48-p	TDIGFWFsQEDFMLK
0	1		K154	NDITAVTKEGKILLT
0	1		S655-p	YSKDCEGsALLKKAL
0	1		K693-a	GNLNELGkMIMQGGF
0	1		K708-a	SVWIGHKkGAtKMKD
0	1		T711-p	IGHKkGAtKMKDLAR
0	1		Y787-p	YGEKEEVyIVQAsNV
0	1		S792-p	EVyIVQAsNVDVKMT

	MCF2 iso3
S108	TDIGFWFSQEDFMLK
K214	NDITAVTKEGKILLT
S715	YSKDCEGSALLKKAL
K753	GNLNELGKMIMQGGF
K768	SVWIGHKKGATKMKD
T771	IGHKKGATKMKDLAR
Y847	YGEKEEVYIVQASNV
S852	EVYIVQASNVDVKMT

	mouse
S48	TDIGFRFSQEDFMLK
K154-u	NDLTAVTkEGKVLLM
T670	YSKEGEGTTKLKEAL
K708	GNINELGKMVLQGSF
K723	NVWLGHRKGATKMKD
T726	LGHRKGATKMKDFAR
Y802	YSEKEEIYIVQAPNV
P807	EIYIVQAPNVDVKML

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