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Protein Page:
MCF2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MCF2 Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42. Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4- phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1. Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis. Belongs to the MCF2 family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Apoptosis; GEFs; Cell development/differentiation; Motility/polarity/chemotaxis; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: Xq27
Cellular Component: cytoskeleton; membrane; cytosol
Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding
Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; dendrite development; positive regulation of Rho GTPase activity
Reference #:  P10911 (UniProtKB)
Alt. Names/Synonyms: ARHGEF21; DBL; DBL-transforming protein; MCF.2 cell line derived transforming sequence; MCF2; MCF2-transforming protein; Oncogene MCF2 (oncogene DBL); Proto-oncogene DBL; Proto-oncogene MCF-2
Gene Symbols: MCF2
Molecular weight: 107,673 Da
Basal Isoelectric point: 5.72  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MCF2

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S48-p TDIGFWFsQEDFMLK
0 1 S130-p ELPDDIPsIEEILAI
0 1 K154 NDITAVTKEGKILLT
1 0 Y510-p LIQTERVyVRELYTV
0 1 S655-p YSKDCEGsALLKKAL
0 1 K693-ac GNLNELGkMIMQGGF
0 1 K708-ac SVWIGHKkGAtKMKD
0 1 T711-p IGHKkGAtKMKDLAR
0 1 Y787-p YGEKEEVyIVQAsNV
0 1 S792-p EVyIVQAsNVDVKMT
  MCF2 iso3  
S108 TDIGFWFSQEDFMLK
S190 ELPDDIPSIEEILAI
K214 NDITAVTKEGKILLT
Y570 LIQTERVYVRELYTV
S715 YSKDCEGSALLKKAL
K753 GNLNELGKMIMQGGF
K768 SVWIGHKKGATKMKD
T771 IGHKKGATKMKDLAR
Y847 YGEKEEVYIVQASNV
S852 EVYIVQASNVDVKMT
  mouse

 
S48 TDIGFRFSQEDFMLK
S130 ELPQDILSIEEILAG
K154-ub NDLTAVTkEGKVLLM
Y525 LIQTERAYVRELFTV
T670 YSKEGEGTTKLKEAL
K708 GNINELGKMVLQGSF
K723 NVWLGHRKGATKMKD
T726 LGHRKGATKMKDFAR
Y802 YSEKEEIYIVQAPNV
P807 EIYIVQAPNVDVKML
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