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Protein Page:
USP14 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
USP14 Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily. Note: This description may include information from UniProtKB.
Protein type: EC 3.4.19.12; Ubiquitin-specific protease; EC 3.1.2.15; Ubiquitin conjugating system; Protease
Cellular Component: proteasome complex; cell surface; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; synapse
Molecular Function: ubiquitin thiolesterase activity; tRNA guanylyltransferase activity; protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; endopeptidase inhibitor activity
Biological Process: ubiquitin-dependent protein catabolic process; synaptic transmission; regulation of chemotaxis; protein deubiquitination
Reference #:  P54578 (UniProtKB)
Alt. Names/Synonyms: Deubiquitinating enzyme 14; TGT; tRNA-guanine transglycosylase, 60-kD subunit; Ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14; ubiquitin specific protease 14 (tRNA-guanine transglycosylase); Ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14; UBP14; USP14
Gene Symbols: USP14
Molecular weight: 56,069 Da
Basal Isoelectric point: 5.2  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

USP14

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 21 Y4-p ____MPLySVTVKWG
0 1 K14-ac TVKWGKEkFEGVELN
0 1 K14 TVKWGKEKFEGVELN
0 1 K49-ac ARQKVMVkGGtLkDD
0 2 K49-ub ARQKVMVkGGtLkDD
0 6 T52-p KVMVkGGtLkDDDWG
0 2 K54 MVkGGtLKDDDWGNI
0 1 K54-sc MVkGGtLkDDDWGNI
0 1 K62 DDDWGNIKIKNGMTL
0 6 K62-ub DDDWGNIkIKNGMTL
0 1 K64 DWGNIkIKNGMTLLM
0 3 K130-ub IRSVPELkDALKRyA
0 1 Y136-p LkDALKRyAGALRAs
0 27 S143-p yAGALRAsGEMAsAQ
0 4 S148-p RAsGEMAsAQyITAA
0 1 Y151-p GEMAsAQyITAALRD
0 6 K214-ub MMRVLQQkLEAIEDD
0 2 S222-p LEAIEDDsVkETDsS
0 8 K224-ub AIEDDsVkETDsSSA
0 1 T226 EDDsVkETDsSSAsA
0 2 S228-p DsVkETDsSSAsAAt
0 2 S232-p ETDsSSAsAAtPskk
1 18 T235-p sSSAsAAtPskkKSL
0 1 S237-p SAsAAtPskkKSLID
0 1 K238-ub AsAAtPskkKSLIDQ
0 3 K239-ub sAAtPskkKSLIDQF
0 5 K269-ub EEEVTKGkENQLQLs
0 2 S276-p kENQLQLsCFINQEV
0 1 K284-ac CFINQEVkyLFTGLk
0 2 K284-ub CFINQEVkyLFTGLk
0 4 Y285-p FINQEVkyLFTGLkL
0 1 K291-ac kyLFTGLkLRLQEEI
0 37 K291-ub kyLFTGLkLRLQEEI
0 1 K291-sc kyLFTGLkLRLQEEI
0 1 T299-p LRLQEEItkQsPTLQ
0 34 K300-ub RLQEEItkQsPTLQR
0 1 S302-p QEEItkQsPTLQRNA
0 1 K313-ac QRNALYIkssKISRL
0 9 K313-ub QRNALYIkssKISRL
0 1 S314-p RNALYIkssKISRLP
0 1 S315-p NALYIkssKISRLPA
0 4 K342-ub EKESVNAkVLKDVkF
0 1 K348-ac AkVLKDVkFPLMLDM
0 5 S393-p PNTSDKKssPQKEVK
0 1 S394-p NTSDKKssPQKEVKY
0 1 S412-p SFADDIGsNNCGyYD
0 1 Y417-p IGsNNCGyYDLQAVL
0 1 S432-p THQGRSSsSGHyVSW
0 1 Y436-p RSSsSGHyVSWVkRk
0 27 K441-ub GHyVSWVkRkQDEWI
0 5 K443-ub yVSWVkRkQDEWIkF
0 2 K449-ac RkQDEWIkFDDDkVS
0 9 K449-ub RkQDEWIkFDDDkVS
0 1 K454-ac WIkFDDDkVSIVtPE
0 4 K454-ub WIkFDDDkVSIVtPE
0 1 T459-p DDkVSIVtPEDILRL
  mouse

 
Y4 ____MPLYSVTVKWG
K14 TVKWGKEKFEGVELN
K14-ub TVKWGKEkFEGVELN
K49 ARQKVMVKGGtLkDD
K49-ub ARQKVMVkGGtLkDD
T52-p KVMVkGGtLkDDDWG
K54-ac MVkGGtLkDDDWGNI
K54 MVkGGtLKDDDWGNI
K62-ac DDDWGNIkMkNGMTV
K62-ub DDDWGNIkMkNGMTV
K64-ub DWGNIkMkNGMTVLM
K130-ub IRSVPELkDALKRyA
Y136-p LkDALKRyAGALRAs
S143-p yAGALRAsGEMAsAQ
S148-p RAsGEMAsAQYITAA
Y151 GEMAsAQYITAALRD
K214-ub MMRVLQQkLEAIEDD
S222-p LEAIEDDsGREtDSS
R224 AIEDDsGREtDSSSA
T226-p EDDsGREtDSSSAPA
S228 DsGREtDSSSAPAVt
P232 EtDSSSAPAVtPSKk
T235-p SSSAPAVtPSKkKSL
S237 SAPAVtPSKkKSLID
K238 APAVtPSKkKSLIDQ
K239-ub PAVtPSKkKSLIDQY
K269 EEEVTKGKENQLQLS
S276 KENQLQLSCFINQEV
K284 CFINQEVKYLFTGLk
K284 CFINQEVKYLFTGLk
Y285 FINQEVKYLFTGLkL
K291 KYLFTGLKLRLQEEI
K291-ub KYLFTGLkLRLQEEI
K291 KYLFTGLKLRLQEEI
T299 LRLQEEITkQSPTLQ
K300-ub RLQEEITkQSPTLQR
S302 QEEITkQSPTLQRNA
K313 QRNALYIKSSKISRL
K313-ub QRNALYIkSSKISRL
S314 RNALYIkSSKISRLP
S315 NALYIkSSKISRLPA
K342-ub EKESVNAkVLKDVKF
K348 AkVLKDVKFPLMLDV
S393-p PNANDKNsPPKEIKY
P394 NANDKNsPPKEIKYE
S411 SFADDIGSNNCGYYD
Y416 IGSNNCGYYDLQAVL
S431 THQGRSSSSGHYVSW
Y435 RSSSSGHYVSWVRRK
R440 GHYVSWVRRKQDEWI
K442 YVSWVRRKQDEWIkF
K448-ac RKQDEWIkFDDDkVS
K448-ub RKQDEWIkFDDDkVS
K453 WIkFDDDKVSIVTPE
K453-ub WIkFDDDkVSIVTPE
T458 DDkVSIVTPEDILRL
  rat

 
Y4 ____MPLYSVTVKWG
K14-ac TVKWGKEkFEGVELN
K14 TVKWGKEKFEGVELN
K49 ARQKVMVKGGTLKDD
K49 ARQKVMVKGGTLKDD
T52 KVMVKGGTLKDDDWG
K54 MVKGGTLKDDDWGNI
K54 MVKGGTLKDDDWGNI
K62 DDDWGNIKMKNGMTV
K62 DDDWGNIKMKNGMTV
K64 DWGNIKMKNGMTVLM
K130 IRSVPELKDALKRYA
Y136 LKDALKRYAGALRAs
S143-p YAGALRAsGEMASAQ
S148 RAsGEMASAQYITAA
Y151 GEMASAQYITAALRD
K214 MMRVLQQKLEAIEDD
S222 LEAIEDDSARETESS
R224 AIEDDSARETESSSA
T226 EDDSARETESSSASA
S228 DSARETESSSASAVT
S232 ETESSSASAVTPSKK
T235 SSSASAVTPSKKKSL
S237 SASAVTPSKKKSLID
K238 ASAVTPSKKKSLIDQ
K239 SAVTPSKKKSLIDQF
K269 EEEVTKGKENQLQLS
S276 KENQLQLSCFINQEV
K284 CFINQEVKYLFTGLK
K284 CFINQEVKYLFTGLK
Y285 FINQEVKYLFTGLKL
K291 KYLFTGLKLRLQEEI
K291 KYLFTGLKLRLQEEI
K291 KYLFTGLKLRLQEEI
T299 LRLQEEITKQSPTLQ
K300 RLQEEITKQSPTLQR
S302 QEEITKQSPTLQRNA
K313 QRNALYIKSSKISRL
K313 QRNALYIKSSKISRL
S314 RNALYIKSSKISRLP
S315 NALYIKSSKISRLPA
K342 EKESVNAKVLKDVKF
K348 AKVLKDVKFPLMLDV
S393-p PNANDKNsPPKEIKY
P394 NANDKNsPPKEIKYE
S411 SFADDIGSNNCGYYD
Y416 IGSNNCGYYDLQAVL
S431 THQGRSSSSGHYVSW
Y435 RSSSSGHYVSWVKRK
K440 GHYVSWVKRKEDEWI
K442 YVSWVKRKEDEWIKF
K448 RKEDEWIKFDDDKVS
K448 RKEDEWIKFDDDKVS
K453 WIKFDDDKVSIVTPE
K453 WIKFDDDKVSIVTPE
T458 DDKVSIVTPEDILRL
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