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Protein Page:
KCC3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
KCC3 Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. Homomultimer and heteromultimer with other K-Cl cotransporters. Up-regulated by VEGF. Down-regulated by TNF. Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney. Activated by N-ethylmaleimide (NEM). Inhibited by DIOA, bumetanide and furosemide. Inhibited by WNK3. Belongs to the SLC12A transporter family. 6 isoforms of the human protein are produced by alternative promoter. Note: This description may include information from UniProtKB.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family
Chromosomal Location of Human Ortholog: 15q13
Cellular Component: basolateral plasma membrane; integral to membrane; plasma membrane
Molecular Function: potassium ion transmembrane transporter activity; protein kinase binding; potassium:chloride symporter activity
Biological Process: potassium ion import; chloride transport; ion transport; angiogenesis; transmembrane transport; potassium ion transport
Reference #:  Q9UHW9 (UniProtKB)
Alt. Names/Synonyms: ACCPN; DKFZp434D2135; Electroneutral potassium-chloride cotransporter 3; K-Cl cotransporter 3; KCC3; KCC3A; KCC3B; potassium chloride cotransporter 3; potassium chloride cotransporter KCC3a-S3; potassium-chloride transporter-3a; potassium-chloride transporter-3b; S12A6; SLC12A6; solute carrier family 12 (potassium/chloride transporters), member 6; Solute carrier family 12 member 6
Gene Symbols: SLC12A6
Molecular weight: 127,617 Da
Basal Isoelectric point: 6.64  Predict pI for various phosphorylation states
Select Structure to View Below

KCC3

Protein Structure Not Found.


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Sites Implicated In
activity, inhibited: T991‑p, T1048‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K9 HPPETTTKMASVRFM
1 0 T18-p ASVRFMVtPTKIDDI
0 1 - gap
0 1 - gap
0 1 - gap
0 4 S29-p IDDIPGLsDtsPDLS
1 2 T31-p DIPGLsDtsPDLSSR
1 16 S32-p IPGLsDtsPDLSSRS
0 1 S36 sDtsPDLSSRSSSRV
0 1 S37 DtsPDLSSRSSSRVR
0 2 S46 SSSRVRFSsREsVPE
0 3 S47-p SSRVRFSsREsVPET
1 6 S50-p VRFSsREsVPETSRS
0 4 S93 QDVIEDLSQNsItGE
2 5 S96-p IEDLSQNsItGEHsQ
1 1 T98-p DLSQNsItGEHsQLL
1 2 S102-p NsItGEHsQLLDDGH
1 2 S120-p RNAYLNNsNyEEGDE
0 2 Y122-p AYLNNsNyEEGDEyF
0 4 Y128-p NyEEGDEyFDKNLAL
1 0 S147-p MDTRPKVssLLNRMA
1 6 S148-p DTRPKVssLLNRMAN
0 3 Y156-p LLNRMANyTNLtQGA
1 0 T160-p MANyTNLtQGAKEHE
0 1 K176-ub AENITEGkKKPTKTP
0 1 Y321-p MLNNMRVyGtAFLVL
0 1 T323-p NNMRVyGtAFLVLMV
0 1 K394 IDVCSKTKEINNMTV
0 1 K460-ac PKGEIIEkPSAkSSD
0 1 K464-ac IIEkPSAkSSDVLGS
0 3 K600-ub RLLQAIAkDNIIPFL
0 1 T778-p VKHPRLLtFAsQLKA
0 1 S781-p PRLLtFAsQLKAGKG
0 1 S940-p HKVWRKCsIRIFTVA
3 10 T991-p SAYTYERtLMMEQRS
0 2 S1007-p MLRHMRLsKTERDRE
2 2 S1023-p QLVKDRNsMLRLtsI
1 6 T1028-p RNsMLRLtsIGsDED
2 10 S1029-p NsMLRLtsIGsDEDE
1 26 S1032-p LRLtsIGsDEDEETE
0 1 T1038 GsDEDEETETYQEKV
0 1 T1040 DEDEETETYQEKVHM
0 1 Y1041 EDEETETYQEKVHMt
3 7 T1048-p YQEKVHMtWtKDKYM
1 2 T1050-p EKVHMtWtKDKYMAS
1 4 S1064-p SRGQKAKsMEGFQDL
0 1 T1129-p MEFLEVLtEGLERVL
  KCC3 iso2  
- gap
T5-p ___MPHFtVTKVEDP
S19-p PEEGAAAsISQEPSL
S21 EGAAAsISQEPSLAD
K30-ub EPSLADIkARIQDSD
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S42-p DSDEPDLsQNsItGE
S45-p EPDLsQNsItGEHsQ
T47-p DLsQNsItGEHsQLL
S51-p NsItGEHsQLLDDGH
S69 RNAYLNNSNYEEGDE
Y71 AYLNNSNYEEGDEYF
Y77 NYEEGDEYFDKNLAL
S96 MDTRPKVSSLLNRMA
S97 DTRPKVSSLLNRMAN
Y105 LLNRMANYTNLTQGA
T109 MANYTNLTQGAKEHE
K125 AENITEGKKKPTKTP
Y270 MLNNMRVYGTAFLVL
T272 NNMRVYGTAFLVLMV
K343 IDVCSKTKEINNMTV
K409 PKGEIIEKPSAKSSD
K413 IIEKPSAKSSDVLGS
K549 RLLQAIAKDNIIPFL
T727 VKHPRLLTFASQLKA
S730 PRLLTFASQLKAGKG
S889 HKVWRKCSIRIFTVA
T940 SAYTYERTLMMEQRS
S956 MLRHMRLSKTERDRE
S972 QLVKDRNSMLRLTSI
T977 RNSMLRLTSIGSDED
S978 NSMLRLTSIGSDEDE
S981 LRLTSIGSDEDEETE
T987 GSDEDEETETYQEKV
T989 DEDEETETYQEKVHM
Y990 EDEETETYQEKVHMT
T997 YQEKVHMTWTKDKYM
T999 EKVHMTWTKDKYMAS
S1013 SRGQKAKSMEGFQDL
T1078 MEFLEVLTEGLERVL
  mouse

► Hide Isoforms
 
K9-ac HPPEATTkMSSVRFM
T18 SSVRFMVTPTKIDDI
- gap
- gap
- gap
S29-p IDDIPGLsDtsPDLs
T31-p DIPGLsDtsPDLssR
S32-p IPGLsDtsPDLssRS
S36-p sDtsPDLssRSSSRV
S37-p DtsPDLssRSSSRVR
S46-p SSSRVRFssREsVPE
S47-p SSRVRFssREsVPET
S50-p VRFssREsVPETSRS
S93 QDVTEDPSQNSITGE
S96 TEDPSQNSITGEHSQ
T98 DPSQNSITGEHSQLL
S102 NSITGEHSQLLDDGH
S120 RNAYLNNSNYEEGDE
Y122 AYLNNSNYEEGDEYF
Y128 NYEEGDEYFDKNLAL
S147 MDTRPKVSsLLNRMA
S148-p DTRPKVSsLLNRMAN
Y156 LLNRMANYTNLTQGA
T160 MANYTNLTQGAKEHE
K176 AENITEGKKKPTKSP
Y321 MLNNMRVYGTAFLVL
T323 NNMRVYGTAFLVLMV
K394-ac LDICSKTkEVDNMTV
K460 PKGEIIEKPSAKSSD
K464 IIEKPSAKSSDVLGN
K600-ub RLLQAIAkDNIIPFL
T778 VKHPRLLTFASQLKA
S781 PRLLTFASQLKAGKG
S940 HKVWRKCSIRIFTVA
T991-p SAYTYERtLMMEQRS
S1007-p MLRHMRLsKTERDRE
S1023-p QLVKDRNsMLRLtsI
T1028-p RNsMLRLtsIGsDED
S1029-p NsMLRLtsIGsDEDE
S1032-p LRLtsIGsDEDEETE
T1038 GsDEDEETETYQEKV
T1040 DEDEETETYQEKVHM
Y1041 EDEETETYQEKVHMt
T1048-p YQEKVHMtWtKDKYM
T1050-p EKVHMtWtKDKYMAS
S1064-p SRGQKVKsMEGFQDL
T1129 MEFLEVLTEGLERVL
  KCC3 iso2  
- gap
T5 ___MPHFTVTKVEDP
P19 PEEGAAGPLsPEPSS
S21-p EGAAGPLsPEPSSAE
K30 EPSSAEVKARIQDPQ
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S42 DPQEPDPSQNSITGE
S45 EPDPSQNSITGEHSQ
T47 DPSQNSITGEHSQLL
S51 NSITGEHSQLLDDGH
S69 RNAYLNNSNYEEGDE
Y71 AYLNNSNYEEGDEYF
Y77 NYEEGDEYFDKNLAL
S96 MDTRPKVSSLLNRMA
S97 DTRPKVSSLLNRMAN
Y105 LLNRMANYTNLTQGA
T109 MANYTNLTQGAKEHE
K125 AENITEGKKKPTKSP
Y270 MLNNMRVYGTAFLVL
T272 NNMRVYGTAFLVLMV
K343 LDICSKTKEVDNMTV
K409 PKGEIIEKPSAKSSD
K413 IIEKPSAKSSDVLGN
K549 RLLQAIAKDNIIPFL
T727 VKHPRLLTFASQLKA
S730 PRLLTFASQLKAGKG
S889 HKVWRKCSIRIFTVA
T940 SAYTYERTLMMEQRS
S956 MLRHMRLSKTERDRE
S972 QLVKDRNSMLRLTSI
T977 RNSMLRLTSIGSDED
S978 NSMLRLTSIGSDEDE
S981 LRLTSIGSDEDEETE
T987 GSDEDEETETYQEKV
T989 DEDEETETYQEKVHM
Y990 EDEETETYQEKVHMT
T997 YQEKVHMTWTKDKYM
T999 EKVHMTWTKDKYMAS
S1013 SRGQKVKSMEGFQDL
T1078 MEFLEVLTEGLERVL
  rat

 
K9 HPPETTTKMSSVRFM
T18 SSVRFMVTPTKIDDI
- gap
- gap
- gap
S29 IDDIPGLSDTsPDLS
T31 DIPGLSDTsPDLSSR
S32-p IPGLSDTsPDLSSRS
S36 SDTsPDLSSRSSSRV
S37 DTsPDLSSRSSSRVR
S46 SSSRVRFSsREsVPE
S47-p SSRVRFSsREsVPET
S50-p VRFSsREsVPETSRS
N93 QDVTEDPNQNsITGE
S96-p TEDPNQNsITGEHSQ
T98 DPNQNsITGEHSQLL
S102 NsITGEHSQLLDDGH
S120 RNAYLNNSNYEEGDE
Y122 AYLNNSNYEEGDEYF
Y128 NYEEGDEYFDKNLAL
S147 MDTRPKVSSLLNRMA
S148 DTRPKVSSLLNRMAN
Y156 LLNRMANYTNLTQGA
T160 MANYTNLTQGAKEHE
K176 AENITEGKKKPTKSP
Y321 MLNNMRVYGTAFLVL
T323 NNMRVYGTAFLVLMV
K394 LDICSKTKEVNNMTI
K460 PKGEIIEKPSAKSSD
K464 IIEKPSAKSSDVLGN
K600 RLLQAIAKDNIIPFL
T778 VKHPRLLTFASQLKA
S781 PRLLTFASQLKAGKG
S940 HKVWRKCSIRIFTVA
T991-p SAYTYERtLMMEQRS
S1007 MLRHMRLSKTERDRE
S1023 QLVKDRNSMLRLtsI
T1028-p RNSMLRLtsIGsDED
S1029-p NSMLRLtsIGsDEDE
S1032-p LRLtsIGsDEDEEtE
T1038-p GsDEDEEtEtyQEKV
T1040-p DEDEEtEtyQEKVHM
Y1041-p EDEEtEtyQEKVHMT
T1048 yQEKVHMTWTKDKYM
T1050 EKVHMTWTKDKYMAS
S1064 SRGQKVKSMEGFQDL
T1129 MEFLEVLTEGLERVL
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