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Protein Page:
IRF7 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
IRF7 Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88- independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF3. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1; this interaction leads to the proteasome- dependent degradation of IRF7. Interacts with Epstein-Barr virus LF2. Interacts with MYD88 AND TRAF6. By type I interferon (IFN) and viruses. Expressed predominantly in spleen, thymus and peripheral blood leukocytes. In the absence of viral infection, maintained as a monomer in an autoinhibited state and phosphorylation disrupts this autoinhibition leading to the liberation of the DNA- binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: nucleoplasm; cytoplasm; endosome membrane; cytosol; nucleus
Molecular Function: protein binding; DNA binding
Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; establishment of viral latency; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; regulation of adaptive immune response; regulation of MyD88-independent toll-like receptor signaling pathway; positive regulation of interferon-beta production; positive regulation of interferon type I production; interferon type I biosynthetic process; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; regulation of interferon type I production; regulation of immune response; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; response to virus; interferon-alpha production; regulation of MyD88-dependent toll-like receptor signaling pathway; regulation of monocyte differentiation; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immunoglobulin mediated immune response; toll-like receptor 9 signaling pathway; response to DNA damage stimulus; interferon-beta production
Reference #:  Q92985 (UniProtKB)
Alt. Names/Synonyms: Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7; IRF-7H; IRF7; IRF7A
Gene Symbols: IRF7
Molecular weight: 54,278 Da
Basal Isoelectric point: 5.89  Predict pI for various phosphorylation states
CST Pathways:  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

IRF7

Protein Structure Not Found.


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Sites Implicated In
transcription, induced: S477‑p, S479‑p
transcription, inhibited: S471‑p, S472‑p, S483‑p, S487‑p
intracellular localization: S477‑p, S479‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S53-p HFARKDLsEADARIF
0 1 K61 EADARIFKAWAVARG
1 0 K92-ac TAERAGWkTNFRCAL
0 1 K120-ub GDPADPHkVYALSRE
0 4 K341-ub PAELPDQkQLRYTEE
0 1 T391-p PGSASPStPACLLPR
1 0 K444-ub DLSAGRPkEkSLVLV
1 0 K446-ub SAGRPkEkSLVLVkL
1 0 K452-ub EkSLVLVkLEPWLCR
1 0 K452 EkSLVLVKLEPWLCR
0 1 R459 kLEPWLCRVHLEGTQ
3 0 S471-p GTQREGVssLDSSsL
3 0 S472-p TQREGVssLDSSsLs
1 0 S475 EGVssLDSSsLsLCL
5 0 S477-p VssLDSSsLsLCLsS
4 0 S479-p sLDSSsLsLCLsSAN
2 0 S483-p SsLsLCLsSANsLYD
1 0 S484 sLsLCLsSANsLYDD
2 0 S487-p LCLsSANsLYDDIEC
5184 : Phospho-IRF-7 (Ser471/472) Antibody
5184 : Phospho-IRF-7 (Ser471/472) Antibody
12390 : Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb
  mouse

 
D51 HFGRRDLDEEDAQIF
K59-ub EEDAQIFkAWAVARG
K92 RRERRGWKTNFRCAL
K120 GDPVDPHKVYELSRE
K295 PAELPDQKQLHYTET
T345 MGTTGPSTPPQLLER
K398 DLSAGRPKEKTLILV
K400 SAGRPKEKTLILVkL
K406 EKTLILVKLEPWVCk
K406-sm EKTLILVkLEPWVCk
K413-ub kLEPWVCkAYLEGVQ
S425-p GVQREGVssLDsSsL
S426-p VQREGVssLDsSsLG
S429-p EGVssLDsSsLGLCL
S431-p VssLDsSsLGLCLss
G433 sLDsSsLGLCLssTN
S437-p SsLGLCLssTNsLYE
S438-p sLGLCLssTNsLYED
S441-p LCLssTNsLYEDIEH
5184 : Phospho-IRF-7 (Ser471/472) Antibody
5184 : Phospho-IRF-7 (Ser471/472) Antibody
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