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Ago2 (human)

Overview Ago2 the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi). Note: This description may include information from UniProtKB. Protein type: RNA processing; EC 3.1.26.n2 Cellular Component: polysome; mRNA cap complex; cytoplasm; cytosol; ribonucleoprotein complex; nucleus Molecular Function: endoribonuclease activity; protein binding; siRNA binding; translation initiation factor activity; metal ion binding; RNA 7-methylguanosine cap binding Biological Process: negative regulation of translational initiation; Notch signaling pathway; translation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; pre-microRNA processing; miRNA-mediated gene silencing, mRNA cleavage; translational initiation; miRNA-mediated gene silencing, negative regulation of translation; gene expression; post-embryonic development Reference #:  AAL76093 (GenPept) Alt. Names/Synonyms: AGO2; argonaute 2; Argonaute2; eIF-2C 2; eIF2C 2; EIF2C2; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C, 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10 Gene Symbols: EIF2C2 Molecular weight: 96,798 Da Basal Isoelectric point: 9.39  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below Ago2 3LUC - A/C/B=439-575 (human) 3LUD - A/C/B=439-575 (human) 3LUG - A/C/B=439-575 (human) 3LUH - A/C/B=439-575 (human) 3LUJ - A/C/B=439-575 (human) 3LUK - A/C/B=439-575 (human) 3QX8 - A/C/B=439-575 (human) 3QX9 - A/C/B=439-575 (human) 4EI1 - A=1-859 (human) 4EI3 - A=1-859 (human) 4F3T - A=1-859 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 3LUC 3LUD 3LUG 3LUH 3LUJ 3LUK 3QX8 3QX9 4EI1 4EI3 4F3T  |  Phospho3D  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
inhibition: Y521‑p intracellular localization: S379‑p, Y521‑p molecular association, regulation: Y521‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	2		S145-p	ALSGRLPsVPFETIQ
0	1		T150	LPsVPFETIQALDVV
0	1		S163	VVMRHLPSMRYTPVG
0	2		K240-u	KSIEEQQkPLTDsQR
1	1		S245-p	QQkPLTDsQRVKFTK
0	1		R247	kPLTDsQRVKFTKEI
1	1		T295-p	LQQESGQtVECtVAQ
1	1		T299-p	SGQtVECtVAQYFKD
0	1		K309-a	QYFKDRHkLVLRYPH
0	3		Y330-p	GQEQKHTyLPLEVCN
0	3		S377-p	EISKLMRsAsFNtDP
2	23		S379-p	SKLMRsAsFNtDPyV
0	1		T382-p	MRsAsFNtDPyVREF
1	1		Y385-p	AsFNtDPyVREFGIM
0	2		K417-u	ILYGGRNkAIATPVQ
0	1		T518-p	VVILPGKtPVyAEVK
1	1		Y521-p	LPGKtPVyAEVKRVG
0	3		K712-u	TRLFCTDkNERVGkS
0	2		K718-u	DkNERVGkSGNIPAG
0	1		Y741-p	HPTEFDFyLCsHAGI
0	1		S744-p	EFDFyLCsHAGIQGt
0	1		T751-p	sHAGIQGtsRPSHYH
0	1		S752-p	HAGIQGtsRPSHYHV
1	1		S790-p	VRCTRSVsIPAPAYY
0	1		S816-p	LVDKEHDsAEGSHTS
0	1		S823	sAEGSHTSGQSNGRD
0	1		K836-u	RDHQALAkAVQVHQD

	mouse
S154-p	ALSGRLPsVPFEtIQ
T159-p	LPsVPFEtIQALDVV
S172-p	VVMRHLPsMRYTPVG
K249-u	KSIEEQQkPLTDSQr
S254	QQkPLTDSQrVKFTK
R256-m2	kPLTDSQrVKFTKEI
T304	LQQESGQTVECTVAQ
T308	SGQTVECTVAQYFKD
K318	QYFKDRHKLVLRYPH
Y339	GQEQKHTYLPLEVCN
S386-p	EISKLMRsAsFNTDP
S388-p	SKLMRsAsFNTDPYV
T391	MRsAsFNTDPYVREF
Y394	AsFNTDPYVREFGIM
K426-u	ILYGGRNkAIATPVQ
T527	VVILPGKTPVYAEVK
Y530	LPGKTPVYAEVKRVG
K721-u	TRLFCTDkNERVGkS
K727-u	DkNERVGkSGNIPAG
Y750	HPTEFDFYLCSHAGI
S753	EFDFYLCSHAGIQGT
T760	SHAGIQGTSRPSHYH
S761	HAGIQGTSRPSHYHV
S799	VRCTRSVSIPAPAYY
S825-p	LVDKEHDsAEGSHTs
S832-p	sAEGSHTsGQSNGRD
K845	RDHQALAKAVQVHQD

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