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Protein Page:
PDCD8 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PDCD8 Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Oxidoreductase; EC 1.-.-.-; Mitochondrial; Apoptosis
Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus
Molecular Function: oxidoreductase activity, acting on NADH or NADPH; protein binding; FAD binding; DNA binding; electron carrier activity; NAD(P)H oxidase activity
Biological Process: chromosome condensation; caspase activation; neuron differentiation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; cell redox homeostasis; positive regulation of apoptosis; apoptosis; DNA fragmentation during apoptosis; DNA catabolic process
Reference #:  O95831 (UniProtKB)
Alt. Names/Synonyms: AIF; AIFM1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; MGC111425; PDCD8; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; striatal apoptosis-inducing factor
Gene Symbols: AIFM1
Molecular weight: 66,901 Da
Basal Isoelectric point: 9.04  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  Mitochondrial Control of Apoptosis
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PDCD8

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 T105-p RISGLGLtPEQkQkK
0 5 K109-u LGLtPEQkQkKAALs
0 3 K111-u LtPEQkQkKAALsAs
0 1 S116-p kQkKAALsAsEGEEV
0 1 S118-p kKAALsAsEGEEVPQ
0 2 K189-u SDDPNVTkTLRFKQW
0 14 S268-p GGTPRSLsAIDRAGA
0 1 S279-p RAGAEVKsRTTLFRK
0 1 S292-p RKIGDFRsLEkISRE
0 1 K295-a GDFRsLEkISREVKS
0 2 T328-p RKARALGtEVIQLFP
0 3 S371-p MPNAIVQsVGVssGK
0 2 S375-p IVQsVGVssGKLLIK
0 1 S376-p VQsVGVssGKLLIKL
0 1 S416 TGGLEIDSDFGGFRV
0 3 Y443-p AGDAACFyDIKLGRR
0 2 K518-u ATAQDNPksATEQSG
0 1 S519-p TAQDNPksATEQSGT
0 5 K593-a PIARKIIkDGEQHED
  mouse

 
S104 RVMGLGLSPEEkQRR
K108-u LGLSPEEkQRRAIAs
R110 LSPEEkQRRAIAsAt
S115-p kQRRAIAsAtEGGSV
T117-p RRAIAsAtEGGSVPQ
K188-u SDDPNVTkTLQFRQW
S267-p GGTPRSLsAIDRAGA
S278 RAGAEVKSRTTLFRK
A291 RKIGDFRALEKISRE
K294 GDFRALEKISREVKS
I327 RKSQASGIEVIQLFP
S370-p MPNAIVQsVGVsGGR
S374-p IVQsVGVsGGRLLIK
G375 VQsVGVsGGRLLIKL
S415-p TGGLEIDsDFGGFRV
Y442 AGDAACFYDIKLGRR
K517-u ATAQDNPksATEQSG
S518-p TAQDNPksATEQSGT
K592-a PIARKIIkDGEQHED
  rat

 
S104 RIMGLGLSPEEKQRR
K108 LGLSPEEKQRRAIAS
R110 LSPEEKQRRAIASAA
S115 KQRRAIASAAEGGSV
- gap
K188 SDDPNVTKTLQFRQW
S267 GGTPRSLSAIDRAGA
S278 RAGAEVKSRTTLFRK
A291 RKIGDFRALEKISRE
K294 GDFRALEKISREVKS
I327 RKSQASGIEVIQLFP
S370 MPNAIVQSVGVSGGK
S374 IVQSVGVSGGKLLIK
G375 VQSVGVSGGKLLIKL
S415 TGGLEIDSDFGGFRV
Y442 AGDAACFYDIKLGRR
K517 ATAQDNPKSATEQSG
S518 TAQDNPKSATEQSGT
K592 PIARKIIKDGEQHED
  pig

 
T105 RIAGLGLTPEEKQKR
K109 LGLTPEEKQKRATSS
K111 LTPEEKQKRATSSAP
S116 KQKRATSSAPEGEPV
- gap
K189 SDDPNVTKTLRFRQW
S268-p GGTPRSLsAIDRAGA
S279 RAGAEVKSRTTLFRK
T292 RKIGDFRTLEKISRE
K295 GDFRTLEKISREVKS
T328 RKARALGTEVIQLFP
S371-p LPNAIVQsVGVSGGK
S375 IVQsVGVSGGKLLIK
G376 VQsVGVSGGKLLIKL
S416 TGGLEIDSDFGGFRV
Y443 AGDAACFYDIKLGRR
K518 ATAQDNPKSATEQSG
S519 TAQDNPKSATEQSGT
K593 PIARKIIKDGEQHED
  cat

 
T79 RMSGLGLTPEEKQKM
K83 LGLTPEEKQKMATSP
K85 LTPEEKQKMATSPAP
- gap
- gap
K163 SDDPNVTKTLRFRQW
S242 GGTPRSLSAIDRAGA
S253 RAGAEVKSRTTLFRK
- gap
- gap
T302 XXARALGTEVIQLFP
S345 LPNAIVQSVGVSGGK
S349 IVQSVGVSGGKLLIK
G350 VQSVGVSGGKLLIKL
S390 TGGLEIDSDFGGFRV
Y417 VRDAACFYDIKLGRR
K492 ATAQDNPKSATEQSG
S493 TAQDNPKSATEQSGT
K567 PIARKIIKDGEQHED
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