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Protein Page:
PDCD8 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PDCD8 Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 1.-.-.-; Apoptosis; Oxidoreductase; Mitochondrial
Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus
Molecular Function: protein dimerization activity; oxidoreductase activity, acting on NADH or NADPH; protein binding; DNA binding; FAD binding; electron carrier activity; NAD(P)H oxidase activity
Biological Process: chromosome condensation; neuron differentiation; caspase activation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; positive regulation of apoptosis; cell redox homeostasis; apoptosis; DNA fragmentation during apoptosis; DNA catabolic process
Reference #:  O95831 (UniProtKB)
Alt. Names/Synonyms: AIF; AIFM1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; MGC111425; PDCD8; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; striatal apoptosis-inducing factor
Gene Symbols: AIFM1
Molecular weight: 66,901 Da
Basal Isoelectric point: 9.04  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  Mitochondrial Control of Apoptosis
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PDCD8

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S56-p QMTRQMAssGAsGGK
0 1 S57-p MTRQMAssGAsGGKI
0 1 S60-p QMAssGAsGGKIDNS
0 1 K89 AYAYKTMKEDEKRYN
0 2 T105-p RISGLGLtPEQkQkK
0 5 K109-ub LGLtPEQkQkKAALs
0 3 K111-ub LtPEQkQkKAALsAs
0 2 S116-p kQkKAALsAsEGEEV
0 2 S118-p kKAALsAsEGEEVPQ
0 1 T188-p FSDDPNVtktLRFKQ
0 2 K189-ub SDDPNVtktLRFKQW
0 1 T190-p DDPNVtktLRFKQWN
0 1 K199 RFKQWNGKERSIYFQ
0 1 K231 GVAVLTGKKVVQLDV
0 1 K231 GVAVLTGKKVVQLDV
0 1 K232 VAVLTGKKVVQLDVR
0 2 K244-ac DVRDNMVkLNDGSQI
0 1 T263-p CLIATGGtPRSLsAI
0 17 S268-p GGtPRSLsAIDRAGA
0 1 S279-p RAGAEVKsRTTLFRK
0 1 S292-p RKIGDFRsLEkISRE
0 1 K295-ac GDFRsLEkISREVKS
0 2 T328-p RKARALGtEVIQLFP
0 1 K337-ac VIQLFPEkGNMGKIL
0 4 S371-p MPNAIVQsVGVssGK
0 2 S375-p IVQsVGVssGKLLIK
0 1 S376-p VQsVGVssGKLLIKL
0 1 S416 TGGLEIDSDFGGFRV
0 4 Y443-p AGDAACFyDIKLGRR
0 1 S500 EAIGLVDSSLPTVGV
0 1 S501 AIGLVDSSLPTVGVF
0 1 T504 LVDSSLPTVGVFAKA
0 2 K518-ub ATAQDNPksAtEQsG
0 2 S519-p TAQDNPksAtEQsGt
0 1 T521-p QDNPksAtEQsGtGI
0 1 S524-p PksAtEQsGtGIRsE
0 1 T526-p sAtEQsGtGIRsEsE
0 1 S530-p QsGtGIRsEsETESE
0 1 S532-p GtGIRsEsETESEAS
0 1 T534 GIRsEsETESEASEI
0 6 K593-ac PIARKIIkDGEQHED
0 1 K593-sc PIARKIIkDGEQHED
  mouse

 
S55 QMARQMASSGSSGGK
S56 MARQMASSGSSGGKM
S59 QMASSGSSGGKMDNS
K88-sc AYAYKTIkEDQKRYN
S104 RVMGLGLSPEEkQRR
K108-ub LGLSPEEkQRRAIAs
R110 LSPEEkQRRAIAsAt
S115-p kQRRAIAsAtEGGSV
T117-p RRAIAsAtEGGSVPQ
T187 FSDDPNVTkTLQFRQ
K188-ub SDDPNVTkTLQFRQW
T189 DDPNVTkTLQFRQWN
K198-sc QFRQWNGkERSIYFQ
K230-ac GVAVLTGkkVVHLDV
K230-sc GVAVLTGkkVVHLDV
K231-ac VAVLTGkkVVHLDVR
K243-ac DVRGNMVkLNDGSQI
T262 CLIATGGTPRSLsAI
S267-p GGTPRSLsAIDRAGA
S278 RAGAEVKSRTTLFRK
A291 RKIGDFRALEKISRE
K294 GDFRALEKISREVKS
I327 RKSQASGIEVIQLFP
K336-ac VIQLFPEkGNMGKIL
S370-p MPNAIVQsVGVsGGR
S374-p IVQsVGVsGGRLLIK
G375 VQsVGVsGGRLLIKL
S415-p TGGLEIDsDFGGFRV
Y442 AGDAACFYDIKLGRR
S499-p EAIGLVDssLPtVGV
S500-p AIGLVDssLPtVGVF
T503-p LVDssLPtVGVFAKA
K517-ub ATAQDNPksATEQSG
S518-p TAQDNPksATEQSGT
T520 QDNPksATEQSGTGI
S523 PksATEQSGTGIRSE
T525 sATEQSGTGIRSESE
S529 QSGTGIRSESEtESE
S531 GTGIRSESEtESEAS
T533-p GIRSESEtESEASEI
K592-ac PIARKIIkDGEQHED
K592-sc PIARKIIkDGEQHED
  rat

 
S55 QMARQMASSGPSGGK
S56 MARQMASSGPSGGKM
S59 QMASSGPSGGKMDNS
K88 AYAYKTIKEDQKRYN
S104 RIMGLGLSPEEKQRR
K108 LGLSPEEKQRRAIAS
R110 LSPEEKQRRAIASAA
S115 KQRRAIASAAEGGSV
- gap
T187 FSDDPNVTKTLQFRQ
K188 SDDPNVTKTLQFRQW
T189 DDPNVTKTLQFRQWN
K198 QFRQWNGKERSIYFQ
K230 GVAVLTGKKVVHLDV
K230 GVAVLTGKKVVHLDV
K231 VAVLTGKKVVHLDVR
K243 DVRGNMVKLNDGSQI
T262 CLIATGGTPRSLSAI
S267 GGTPRSLSAIDRAGA
S278 RAGAEVKSRTTLFRK
A291 RKIGDFRALEKISRE
K294 GDFRALEKISREVKS
I327 RKSQASGIEVIQLFP
K336 VIQLFPEKGNMGKIL
S370 MPNAIVQSVGVSGGK
S374 IVQSVGVSGGKLLIK
G375 VQSVGVSGGKLLIKL
S415 TGGLEIDSDFGGFRV
Y442 AGDAACFYDIKLGRR
S499 EAIGLVDSSLPTVGV
S500 AIGLVDSSLPTVGVF
T503 LVDSSLPTVGVFAKA
K517 ATAQDNPKSATEQSG
S518 TAQDNPKSATEQSGT
T520 QDNPKSATEQSGTGI
S523 PKSATEQSGTGIRSE
T525 SATEQSGTGIRSESE
S529 QSGTGIRSESETESE
S531 GTGIRSESETESEAS
T533 GIRSESETESEASEI
K592 PIARKIIKDGEQHED
K592 PIARKIIKDGEQHED
  pig

 
S56 QMTRQMASSGASGGK
S57 MTRQMASSGASGGKI
S60 QMASSGASGGKIDNT
K89 AYAYRTIKDNKKRYN
T105 RIAGLGLTPEEKQKR
K109 LGLTPEEKQKRATSS
K111 LTPEEKQKRATSSAP
S116 KQKRATSSAPEGEPV
- gap
T188 FSDDPNVTKTLRFRQ
K189 SDDPNVTKTLRFRQW
T190 DDPNVTKTLRFRQWN
K199 RFRQWNGKERSIYFQ
K231 GVAVLTGKKVVQLDV
K231 GVAVLTGKKVVQLDV
K232 VAVLTGKKVVQLDVR
K244 DVRGNMAKLNDGSQI
T263 CLIATGGTPRSLsAI
S268-p GGTPRSLsAIDRAGA
S279 RAGAEVKSRTTLFRK
T292 RKIGDFRTLEKISRE
K295 GDFRTLEKISREVKS
T328 RKARALGTEVIQLFP
K337 VIQLFPEKGNMGKIL
S371-p LPNAIVQsVGVSGGK
S375 IVQsVGVSGGKLLIK
G376 VQsVGVSGGKLLIKL
S416 TGGLEIDSDFGGFRV
Y443 AGDAACFYDIKLGRR
S500 EAIGLVDSSLPTVGV
S501 AIGLVDSSLPTVGVF
T504 LVDSSLPTVGVFAKA
K518 ATAQDNPKSATEQSG
S519 TAQDNPKSATEQSGT
T521 QDNPKSATEQSGTGI
S524 PKSATEQSGTGIRSE
T526 SATEQSGTGIRSESE
S530 QSGTGIRSESETESE
S532 GTGIRSESETESEAP
T534 GIRSESETESEAPEI
K593 PIARKIIKDGEQHED
K593 PIARKIIKDGEQHED
  cat

 
S29 SPSRSLASTGAPVKD
T30 PSRSLASTGAPVKDG
P33 SLASTGAPVKDGSSL
K63 YYAYKTIKDDQKRYN
T79 RMSGLGLTPEEKQKM
K83 LGLTPEEKQKMATSP
K85 LTPEEKQKMATSPAP
- gap
- gap
T162 FSDDPNVTKTLRFRQ
K163 SDDPNVTKTLRFRQW
T164 DDPNVTKTLRFRQWN
K173 RFRQWNGKERSIYFQ
K205 GVAVLTGKKVVQLDV
K205 GVAVLTGKKVVQLDV
K206 VAVLTGKKVVQLDVR
K218 DVRGNMVKLNDGSQI
T237 CLLATGGTPRSLSAI
S242 GGTPRSLSAIDRAGA
S253 RAGAEVKSRTTLFRK
- gap
- gap
T302 XXARALGTEVIQLFP
K311 VIQLFPEKGNMGKIL
S345 LPNAIVQSVGVSGGK
S349 IVQSVGVSGGKLLIK
G350 VQSVGVSGGKLLIKL
S390 TGGLEIDSDFGGFRV
Y417 VRDAACFYDIKLGRR
S474 EAIGLVDSSLPTVGV
S475 AIGLVDSSLPTVGVF
T478 LVDSSLPTVGVFAKA
K492 ATAQDNPKSATEQSG
S493 TAQDNPKSATEQSGT
T495 QDNPKSATEQSGTGI
S498 PKSATEQSGTGIRSE
T500 SATEQSGTGIRSESE
S504 QSGTGIRSESETESE
S506 GTGIRSESETESEAS
T508 GIRSESETESEASEI
K567 PIARKIIKDGEQHED
K567 PIARKIIKDGEQHED
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