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Protein Page:
eIF2B-epsilon (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
eIF2B-epsilon eukaryotic translation initiation factor 2B, subunit 5. A translational regulatory protein that functions in the early steps of protein synthesis by catalyzing the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy. Note: This description may include information from UniProtKB.
Protein type: Translation initiation; Translation
Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; translation initiation factor binding; translation initiation factor activity; guanyl-nucleotide exchange factor activity
Biological Process: myelination; response to peptide hormone stimulus; translation; astrocyte development; positive regulation of translational initiation; cellular protein metabolic process; ovarian follicle development; astrocyte differentiation; response to heat; translational initiation; response to glucose stimulus; negative regulation of translation initiation in response to stress; gene expression; oligodendrocyte development; positive regulation of GTPase activity
Reference #:  Q13144 (UniProtKB)
Alt. Names/Synonyms: CACH; CLE; EI2BE; EIF-2B; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B5; EIF2BE; EIF2Bepsilon; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; LVWM; Translation initiation factor eIF-2B subunit epsilon
Gene Symbols: EIF2B5
Molecular weight: 80,380 Da
Basal Isoelectric point: 4.98  Predict pI for various phosphorylation states
CST Pathways:  Translation: eIF2  |  Translational Control
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

eIF2B-epsilon

Protein Structure Not Found.


STRING  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  DISEASE  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
translation, altered: S525‑p
activity, induced: S525‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 P4 ____MAAPVVAPPGV
0 2 N17 GVVVSRANKRsGAGP
0 1 R19 VVSRANKRsGAGPGG
0 1 S20-p VSRANKRsGAGPGGs
1 1 S27-p sGAGPGGsGGGGARG
1 0 K61 RRFFPISKDQPRVLL
1 0 K103 CWKAAQIKEHLLKSK
1 0 S130 ITSELYRSLGDVLRD
1 1 K141-ub VLRDVDAkALVRSDF
0 1 T182-p EKNVSVMtMIFKESS
1 0 K217 NRVLHFQKTQGLRRF
0 1 S229 RRFAFPLSLFQGSSD
0 3 Y319-p DVIRRWVyPLTPEAN
1 0 T322 RRWVyPLTPEANFTD
0 1 S447-p NITLPEGsVIsLHPP
0 1 S450-p LPEGsVIsLHPPDAE
3 3 S466-p DEDDGEFsDDsGADQ
2 3 S469-p DGEFsDDsGADQEKD
0 1 K481 EKDKVKMKGyNPAEV
0 1 Y483-p DKVKMKGyNPAEVGA
0 1 K493-ub AEVGAAGkGYLWKAA
1 0 - gap
1 1 S525-p KINMEEEsEsEsEQs
0 1 S527-p NMEEEsEsEsEQsMD
0 1 S529-p EEEsEsEsEQsMDsE
0 1 S532-p sEsEsEQsMDsEEPD
0 1 S535-p EsEQsMDsEEPDsRG
9 2 S540-p MDsEEPDsRGGsPQM
5 50 S544-p EPDsRGGsPQMDDIK
0 1 Y583-p LEINSLKyAYNISLK
0 1 S610-p FPLQQMDsPLDSSRY
0 1 K626 ALLLPLLKAWSPVFR
3 6 S717-p LKEAEEEssEDD___
3 6 S718-p KEAEEEssEDD____
3596 : Phospho-eIF2B-epsilon (Ser540) Antibody
  mouse

 
T4-p ____MAAtAAVPGAA
S16-p GAAAGRAsKrGGGGS
R18-m1 AAGRAsKrGGGGSGG
G19 AGRAsKrGGGGSGGG
S23 sKrGGGGSGGGGTQG
K57 RRFFPISKDQPRVLL
K99 CWKAAQIKEHLQKSK
S126 ITSELYRSLGDVLRD
K137 VLRDVDAKALVRSDF
T178 EKNVSVMTMVFKESS
K213 NRVLHFQKTQGLRRF
S225-p RRFSFPLsLFQGSGD
Y315 DVIRRWVYPLTPEVN
T318 RRWVYPLTPEVNFTD
S443-p DITLPEGsVIsLHPP
S446-p LPEGsVIsLHPPDAE
S462-p DEDDGQFsDDsGADQ
S465-p DGQFsDDsGADQEKE
K477-ub EKEKVKLkGYNPAEV
Y479 EKVKLkGYNPAEVGL
Q489 AEVGLEGQGYLWKAE
K501 KAEGVNSKEDEELRQ
S521 MIKTEEESETESEGS
T523 KTEEESETESEGSVD
S525 EEESETESEGSVDPE
S528 SETESEGSVDPEELD
P531 ESEGSVDPEELDsRA
S536-p VDPEELDsRAGsPQL
S540-p ELDsRAGsPQLDDIR
H579 LEINSLKHAYNISLK
G606 FPLQQVDGLLDPNRY
K622-ub ALLLPLLkAWSPVLR
S713-p LREAEEEssEDD___
S714-p REAEEEssEDD____
  rat

 
T4 ____MAATAAVPSAV
N16 SAVGGRANKRGGGsG
R18 VGGRANKRGGGsGGG
G19 GGRANKRGGGsGGGG
S22-p ANKRGGGsGGGGTQG
K56-ub RRFFPISkDQPRVLL
K98-ub CWKAAQIkEHLQKSK
S125-p ITSDLYRsLGDVLRD
K136-ub VLRDVDAkALVRSDF
T177 EKNVSVMTMVFKESS
K212-ub NRILHFQkTQGLRHF
G224 RHFSFPLGLFQGSLD
Y314 DVIRRWVYPLtPEVN
T317-p RRWVYPLtPEVNFTD
S442 DIILPEGSVISLHPP
S445 LPEGSVISLHPPDAE
S461-p DEDDGQFsDDsGADQ
S464-p DGQFsDDsGADQEKE
K476 EKEKVKLKGYNPAEV
Y478 EKVKLKGYNPAEVGP
Q488 AEVGPEGQGYLWKAE
K500-ub KAEDVDEkEDEELRQ
S520 MINMEEESETESERS
T522 NMEEESETESERSVD
S524 EEESETESERSVDPE
S527 SETESERSVDPEELD
P530 ESERSVDPEELDsRA
S535-p VDPEELDsRAGsPQL
S539-p ELDsRAGsPQLDDIR
Y578 LEINSLKYAYNISLK
G605 FPLQQVDGVLDPNRY
K621 ALLLPLLKAWSPVFR
S712-p LREAEEEssDDD___
S713-p REAEEEssDDD____
3596 : Phospho-eIF2B-epsilon (Ser540) Antibody
  rabbit

 
T4 ____MATTVVAPPGA
N17 GAVSDRANKRGGGPG
R19 VSDRANKRGGGPGGG
G20 SDRANKRGGGPGGGG
G27 GGGPGGGGGGGGARG
K61 RRFFPISKDQPRVLL
K103 CWKAAQIKEHLQKSK
S130 ITSELYRSLGDVLRD
K141 VLRDVDAKALVRSDF
T182 EKNVSVMTMIFKESS
K217 NRILHFQKTQGLRRF
S229 RRFSFPLSLFQGSGA
Y319 DVIRRWVYPLTPEAN
T322 RRWVYPLTPEANFTD
S447 NITLPEGSVISLHPP
S450 LPEGSVISLHPPDAE
S466 DEDDGQFSDDSGVNQ
S469 DGQFSDDSGVNQAKE
K481 AKEKAKLKGYNPAEV
Y483 EKAKLKGYNPAEVGV
K493 AEVGVAGKGYLWKAA
E505 KAADMNTEKEEELRQ
S525 TINEEEESETESERS
T527 NEEEESETESERSMD
S529 EEESETESERSMDSE
S532 SETESERSMDSEELD
S535 ESERSMDSEELDSRA
S540 MDSEELDSRAGSPQL
S544 ELDSRAGSPQLDDIK
Y583 LEINSLKYAYNISLK
S610 FPLQQMDSPLEANRY
K626 ALLLPLLKAWSPVFR
S717-p LKEAEEEssEDD___
S718-p KEAEEEssEDD____
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