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Protein Page:
HSF1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HSF1 a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Cellular Component: protein complex; pronucleus; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; chromatin binding; transcription factor activity
Biological Process: negative regulation of cell proliferation; embryonic placenta development; transcription, DNA-dependent; embryonic process involved in female pregnancy; female meiosis; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; defense response; spermatogenesis; positive regulation of multicellular organism growth; negative regulation of tumor necrosis factor production; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation
Reference #:  Q00613 (UniProtKB)
Alt. Names/Synonyms: Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSF1; HSTF 1; HSTF1
Gene Symbols: HSF1
Molecular weight: 57,260 Da
Basal Isoelectric point: 5.02  Predict pI for various phosphorylation states
CST Pathways:  SAPK/JNK Signaling Cascades
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HSF1

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: S216‑p
transcription, altered: T142‑p, S303‑p, S326‑p
transcription, induced: S307‑p, S320‑p, S326‑p
transcription, inhibited: K298‑sm, S303‑p, S307‑p
activity, induced: T142‑p, S320‑p, S326‑p
activity, inhibited: S121‑p, S303‑p, S307‑p
intracellular localization: S216‑p, S303‑p, S307‑p, S320‑p
molecular association, regulation: S121‑p, T142‑p, S216‑p, S303‑p, S307‑p
protein degradation: S216‑p, S307‑p
protein processing: S303‑p
sumoylation: S307‑p
ubiquitination: S216‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
2 0 K80-ac LNMYGFRkVVHIEQG
1 0 K118-ac LLENIKRkVTsVSTL
2 3 S121-p NIKRkVTsVSTLKsE
0 1 S127-p TsVSTLKsEDIKIRQ
2 0 T142-p DSVTKLLtDVQLMKG
1 0 K208-ac RILGVKRkIPLMLND
2 3 S216-p IPLMLNDsGsAHSMP
0 1 S218-p LMLNDsGsAHSMPKY
8 5 S230-p PKYSRQFsLEHVHGS
1 1 S275-p ITELAPAsPMAsPGG
0 2 S279-p APAsPMAsPGGsIDE
0 1 S283-p PMAsPGGsIDERPLS
2 4 S292-p DERPLSSsPLVRVkE
1 0 K298-ac SsPLVRVkEEPPsPP
4 0 K298-sm SsPLVRVkEEPPsPP
13 30 S303-p RVkEEPPsPPQsPRV
12 26 S307-p EPPsPPQsPRVEEAs
2 24 S314-p sPRVEEAsPGRPssV
1 5 S319-p EAsPGRPssVDtLLs
3 2 S320-p AsPGRPssVDtLLsP
0 6 T323-p GRPssVDtLLsPTAL
9 22 S326-p ssVDtLLsPTALIDs
0 2 T328 VDtLLsPTALIDsIL
1 1 S333-p sPTALIDsILRESEP
1 3 S344-p ESEPAPAsVTALTDA
0 1 T355-p LTDARGHtDtEGRPP
0 3 T357-p DARGHtDtEGRPPsP
1 49 S363-p DtEGRPPsPPPtstP
0 11 T367-p RPPsPPPtstPEKCL
0 7 S368-p PPsPPPtstPEKCLS
0 37 T369-p PsPPPtstPEKCLSV
0 1 S403 DNLQTMLSSHGFSVD
0 1 S404 NLQTMLSSHGFSVDT
2 1 S419-p SALLDLFsPSVTVPD
1 1 S444-p ASIQELLsPQEPPRP
0 3 S501-p LGEGSYFsEGDGFAE
1 0 K524-ac GSEPPKAkDPTVS__
  mouse

► Hide Isoforms
 
K80 LNMYGFRKVVHIEQG
K118 LLENIKRKVTSVSTL
S121 NIKRKVTSVSTLKSE
S127 TSVSTLKSEDIKIRQ
T142-p DSVTRLLtDVQLMKG
K208 RILGVKRKIPLMLSD
S216 IPLMLSDSNSAHSVP
S218 LMLSDSNSAHSVPKY
S230-p PKYGRQYsLEHVHGP
S275 ITELAPTSPLASPGR
S279 APTSPLASPGRSIDE
S283 PLASPGRSIDERPLS
S292 DERPLSSSTLVRVKQ
K298 SSTLVRVKQEPPsPP
K298 SSTLVRVKQEPPsPP
S303-p RVKQEPPsPPHsPRV
S307-p EPPsPPHsPRVLEAs
S314-p sPRVLEAsPGRPssM
S319-p EAsPGRPssMDtPLs
S320-p AsPGRPssMDtPLsP
T323-p GRPssMDtPLsPtAF
S326-p ssMDtPLsPtAFIDs
T328-p MDtPLsPtAFIDsIL
S333-p sPtAFIDsILRESEP
S345 SEPTPAASNTAPMDT
- gap
- gap
A359 TTGAQAPALPTPStP
P363 QAPALPTPStPEKCL
S364 APALPTPStPEKCLS
T365-p PALPTPStPEKCLSV
T399 DNLQTMLTSHGFSVD
S400 NLQTMLTSHGFSVDT
S415-p SALLDLFsPSVTMPD
S440 ASIQELLSPQEPPRP
S497 LGESSYFSEGDDYTD
K520 GTEPHKAKDPTVS__
  HSF1 iso2  
K80 LNMYGFRKVVHIEQG
K118 LLENIKRKVTSVSTL
S121 NIKRKVTSVSTLKSE
S127 TSVSTLKSEDIKIRQ
T142 DSVTRLLTDVQLMKG
K208 RILGVKRKIPLMLSD
S216 IPLMLSDSNSAHSVP
S218 LMLSDSNSAHSVPKY
S230 PKYGRQYSLEHVHGP
S275 ITELAPTSPLASPGR
S279 APTSPLASPGRSIDE
S283 PLASPGRSIDERPLS
S292 DERPLSSSTLVRVKQ
K298 SSTLVRVKQEPPSPP
K298 SSTLVRVKQEPPSPP
S303 RVKQEPPSPPHSPRV
S307 EPPSPPHSPRVLEAS
S314 SPRVLEASPGRPSSM
S319 EASPGRPSSMDTPLS
S320 ASPGRPSSMDTPLSP
T323 GRPSSMDTPLSPTAF
S326 SSMDTPLSPTAFIDS
T328 MDTPLSPTAFIDSIL
S333 SPTAFIDSILRESEP
S345 SEPTPAASNTAPMDT
- gap
- gap
A359 TTGAQAPALPTPSTP
P363 QAPALPTPSTPEKCL
S364 APALPTPSTPEKCLS
T365 PALPTPSTPEKCLSV
T399-p DNLQTMLtsHGFSVD
S400-p NLQTMLtsHGFSVDT
- gap
S418 LDIQELLSPQEPPRP
S475 LGESSYFSEGDDYTD
K498 GTEPHKAKDPTVS__
  rat

 
K80 LNMYGFRKVVHIEQG
K118 LLENIKRKVTSVSTL
S121 NIKRKVTSVSTLKSE
S127 TSVSTLKSEDIKIRQ
T142 DSVTRLLTDVQLMKG
K208 RILGVKRKIPLMLSD
S216 IPLMLSDSSSAHSVP
S218 LMLSDSSSAHSVPKY
S230-p PKYGRQYsLEHVHGP
S275 ITELAPTSPLASPGR
S279 APTSPLASPGRSIDE
S283 PLASPGRSIDERPLS
S292 DERPLSSSTLVRVKE
K298 SSTLVRVKEEPPsPP
K298 SSTLVRVKEEPPsPP
S303-p RVKEEPPsPPHsPRV
S307-p EPPsPPHsPRVLEAs
S314-p sPRVLEAsPGRPASM
A319 EAsPGRPASMDTPLs
S320 AsPGRPASMDTPLsP
T323 GRPASMDTPLsPTAF
S326-p ASMDTPLsPTAFIDS
T328 MDTPLsPTAFIDSIL
S333 sPTAFIDSILRESEP
S345 SEPTPAASNTAPMDT
- gap
- gap
A359 TTGAQAPAPPAPSTP
P363 QAPAPPAPSTPEKCL
S364 APAPPAPSTPEKCLS
T365 PAPPAPSTPEKCLSV
T399 DNLQTMLTSHGFSVD
S400 NLQTMLTSHGFSVDT
S415 SALLDLFSPSVTMPD
S440 ASIQELLSPQEPPRP
S497 LGESSYFSEGDDYTD
K520 GTEPHKAKDPTVS__
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