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Protein Page:
cortactin (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
cortactin a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein; Actin binding protein; Motility/polarity/chemotaxis
Cellular Component: ruffle; cytoskeleton; lamellipodium; cytoplasm; cell cortex
Molecular Function: protein binding
Reference #:  Q14247 (UniProtKB)
Alt. Names/Synonyms: 1110020L01Rik; Amplaxin; cortactin; CTTN; EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); FLJ34459; Oncogene EMS1; Src substrate cortactin; SRC8
Gene Symbols: CTTN
Molecular weight: 61,586 Da
Basal Isoelectric point: 5.24  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Regulation of Actin Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

cortactin
Protein Structure Not Found.


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Sites Implicated In
apoptosis, inhibited: Y446‑p
carcinogenesis, altered: S405‑p, S418‑p
cell adhesion, altered: S405‑p, S418‑p
cell motility, altered: S298‑p, S405‑p, S418‑p
cytoskeletal reorganization: S298‑p, S405‑p, S418‑p, Y421‑p, Y470‑p, Y486‑p
endocytosis, altered: S405‑p, S418‑p
molecular association, regulation: S298‑p, S405‑p, S418‑p, Y421‑p, Y446‑p, Y470‑p, Y486‑p
phosphorylation: S298‑p
protein conformation: S405‑p, S418‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 S5 ___MWKASAGHAVsI
0 4 S11-p ASAGHAVsIAQDDAG
0 1 A13 AGHAVsIAQDDAGAD
0 6 T24-p AGADDWEtDPDFVND
0 1 S33-p PDFVNDVsEKEQRWG
0 4 T43-p EQRWGAKtVQGSGHQ
0 4 Y84-p GPKASHGyGGkFGVE
1 4 K87-a ASHGyGGkFGVEQDR
0 1 K87-u ASHGyGGkFGVEQDR
0 63 Y104-p KSAVGHEyQSKLsKH
0 2 S109-p HEyQSKLsKHCsQVD
1 11 S113-p SKLsKHCsQVDsVrG
0 1 S117-p KHCsQVDsVrGFGGk
0 3 R119-m1 CsQVDsVrGFGGkFG
1 5 K124-a sVrGFGGkFGVQMDR
0 2 K124-u sVrGFGGkFGVQMDR
0 82 Y141-p QSAVGFEyQGKTEKH
1 4 S150-p GKTEKHAsQKDyssG
0 188 Y154-p KHAsQKDyssGFGGk
0 8 S155-p HAsQKDyssGFGGky
0 23 S156-p AsQKDyssGFGGkyG
1 2 K161-a yssGFGGkyGVQADR
0 1 K161-u yssGFGGkyGVQADR
0 2 Y162-p ssGFGGkyGVQADRV
0 2 K171-a VQADRVDkSAVGFDy
0 1 K171-u VQADRVDkSAVGFDy
0 1 S172 QADRVDkSAVGFDyQ
0 120 Y178-p kSAVGFDyQGktEKH
0 4 K181-a VGFDyQGktEKHESQ
0 2 T182-p GFDyQGktEKHESQR
0 1 Y191-p KHESQRDySKGFGGk
1 2 K198-a ySKGFGGkYGIDkDK
0 1 K198-u ySKGFGGkYGIDkDK
1 0 K203-a GGkYGIDkDKVDkSA
0 1 K208-u IDkDKVDkSAVGFEy
0 72 Y215-p kSAVGFEyQGKtEKH
0 2 T219-p GFEyQGKtEKHESQK
0 44 Y228-p KHESQKDyVKGFGGk
1 13 K235-a yVKGFGGkFGVQTDR
0 1 K235-u yVKGFGGkFGVQTDR
0 5 S261-p EKLQLHEsQKDyKtG
0 103 Y265-p LHEsQKDyKtGFGGk
0 1 T267-p EsQKDyKtGFGGkFG
1 11 K272-a yKtGFGGkFGVQSER
0 1 K272-u yKtGFGGkFGVQSER
1 1 S282-p VQSERQDsAAVGFDy
0 11 Y289-p sAAVGFDykEKLAKH
0 1 K290-a AAVGFDykEKLAKHE
2 4 S298-p EKLAKHEsQQDyskG
0 46 Y302-p KHEsQQDyskGFGGk
0 7 S303-p HEsQQDyskGFGGky
0 1 K304-a EsQQDyskGFGGkyG
1 6 K309-a yskGFGGkyGVQKDR
0 1 K309 yskGFGGKyGVQKDR
0 3 Y310-p skGFGGkyGVQKDRM
1 0 K319-a VQKDRMDkNAstFED
0 2 S322-p DRMDkNAstFEDVTQ
0 2 T323-p RMDkNAstFEDVTQV
1 284 Y334-p VTQVSSAyQkTVPVE
0 2 K336-u QVSSAyQkTVPVEAV
0 1 S345-p VPVEAVTskTsNIRA
0 1 K346-u PVEAVTskTsNIRAN
1 1 S348-p EAVTskTsNIRANFE
0 55 T399-p EEQARAKtQtPPVsP
0 147 T401-p QARAKtQtPPVsPAP
9 152 S405-p KtQtPPVsPAPQPtE
0 30 A407 QtPPVsPAPQPtEER
0 21 T411-p VsPAPQPtEERLPss
0 74 S417-p PtEERLPssPVyEDA
7 218 S418-p tEERLPssPVyEDAA
23 741 Y421-p RLPssPVyEDAAsFK
0 1 A424 ssPVyEDAAsFKAEL
0 7 S426-p PVyEDAAsFKAELsy
0 26 S432-p AsFKAELsyRGPVsG
0 26 Y433-p sFKAELsyRGPVsGt
0 4 S438-p LsyRGPVsGtEPEPV
0 3 T440-p yRGPVsGtEPEPVys
2 901 Y446-p GtEPEPVysMEAADy
0 51 S447-p tEPEPVysMEAADyR
0 213 Y453-p ysMEAADyREASSQQ
0 18 Y464-p SSQQGLAyATEAVyE
15 12 Y470-p AyATEAVyESAEAPG
1 2 Y479-p SAEAPGHyPAEDSty
0 1 T485-p HyPAEDStyDEYEND
16 4 Y486-p yPAEDStyDEYENDL
0 2 Y538-p RGVCKGRyGLFPANy
0 17 Y545-p yGLFPANyVELRQ__
4569 : Phospho-Cortactin (Tyr421) Antibody
  cortactin iso3  
S5 ___MWKASAGHAVSI
S11 ASAGHAVSIAQDDAG
A13 AGHAVSIAQDDAGAD
T24-p AGADDWEtDPDFVND
S33 PDFVNDVSEKEQRWG
T43 EQRWGAKTVQGSGHQ
Y84 GPKASHGYGGKFGVE
K87 ASHGYGGKFGVEQDR
K87 ASHGYGGKFGVEQDR
Y104-p KSAVGHEyQSKLSKH
S109 HEyQSKLSKHCSQVD
S113 SKLSKHCSQVDSVRG
S117 KHCSQVDSVRGFGGK
R119 CSQVDSVRGFGGKFG
K124 SVRGFGGKFGVQMDR
K124 SVRGFGGKFGVQMDR
Y141-p QSAVGFEyQGKTEKH
S150 GKTEKHASQKDySSG
Y154-p KHASQKDySSGFGGK
S155 HASQKDySSGFGGKY
S156 ASQKDySSGFGGKYG
K161 ySSGFGGKYGVQADR
K161 ySSGFGGKYGVQADR
Y162 SSGFGGKYGVQADRV
K171 VQADRVDKSAVGFDy
K171 VQADRVDKSAVGFDy
S172 QADRVDKSAVGFDyQ
Y178-p KSAVGFDyQGkTEKH
K181-a VGFDyQGkTEKHESQ
T182 GFDyQGkTEKHESQR
Y191 KHESQRDYSKGFGGK
K198 YSKGFGGKYGIDKDK
K198 YSKGFGGKYGIDKDK
K203 GGKYGIDKDKVDKSA
K208 IDKDKVDKSAVGFEy
Y215-p KSAVGFEyQGKTEKH
T219 GFEyQGKTEKHESQK
Y228 KHESQKDYVKGFGGK
K235 YVKGFGGKFGVQTDR
K235 YVKGFGGKFGVQTDR
S261-p EKLQLHEsQKDysKG
Y265-p LHEsQKDysKGFGGK
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S266-p HEsQKDysKGFGGKY
K267 EsQKDysKGFGGKYG
K272 ysKGFGGKYGVQKDR
K272 ysKGFGGKYGVQKDR
Y273 sKGFGGKYGVQKDRM
K282 VQKDRMDKNAstFED
S285-p DRMDKNAstFEDVTQ
T286-p RMDKNAstFEDVTQV
Y297-p VTQVSSAyQKTVPVE
K299 QVSSAyQKTVPVEAV
S308 VPVEAVTSKTSNIRA
K309 PVEAVTSKTSNIRAN
S311 EAVTSKTSNIRANFE
T362 EEQARAKTQtPPVsP
T364-p QARAKTQtPPVsPAP
S368-p KTQtPPVsPAPQPTE
A370 QtPPVsPAPQPTEER
T374 VsPAPQPTEERLPss
S380-p PTEERLPssPVyEDA
S381-p TEERLPssPVyEDAA
Y384-p RLPssPVyEDAASFK
A387 ssPVyEDAASFKAEL
S389 PVyEDAASFKAELSy
S395 ASFKAELSyRGPVSG
Y396-p SFKAELSyRGPVSGT
S401 LSyRGPVSGTEPEPV
T403 yRGPVSGTEPEPVyS
Y409-p GTEPEPVySMEAADy
S410 TEPEPVySMEAADyR
Y416-p ySMEAADyREASSQQ
Y427-p SSQQGLAyATEAVYE
Y433 AyATEAVYESAEAPG
Y442 SAEAPGHYPAEDSTY
T448 HYPAEDSTYDEYEND
Y449 YPAEDSTYDEYENDL
Y501 RGVCKGRYGLFPANY
Y508 YGLFPANYVELRQ__
  mouse

 
S5-p ___MWKAsAGHAVsI
S11-p AsAGHAVsItQDDGG
T13-p AGHAVsItQDDGGAD
T24 GGADDWETDPDFVND
S33 PDFVNDVSEKEQRWG
T43 EQRWGAKTVQGSGHQ
Y84 GPKASHGYGGKFGVE
K87 ASHGYGGKFGVEQDR
K87 ASHGYGGKFGVEQDR
Y104-p RSAVGHEyQSKLSKH
S109 HEyQSKLSKHCsQVD
S113-p SKLSKHCsQVDSVrG
S117 KHCsQVDSVrGFGGk
R119-m1 CsQVDSVrGFGGkFG
K124 SVrGFGGKFGVQMDR
K124-u SVrGFGGkFGVQMDR
Y141-p QSAVGFEyQGKTEKH
S150-p GKTEKHAsQKDySSG
Y154-p KHAsQKDySSGFGGK
S155 HAsQKDySSGFGGKY
S156 AsQKDySSGFGGKYG
K161 ySSGFGGKYGVQADR
K161 ySSGFGGKYGVQADR
Y162 SSGFGGKYGVQADRV
K171 VQADRVDKsAVGFDY
K171 VQADRVDKsAVGFDY
S172-p QADRVDKsAVGFDYQ
Y178 KsAVGFDYQGkTEKH
K181-a VGFDYQGkTEKHESQ
T182 GFDYQGkTEKHESQK
Y191 KHESQKDYSKGFGGK
K198 YSKGFGGKYGIDKDK
K198 YSKGFGGKYGIDKDK
K203 GGKYGIDKDKVDKSA
K208 IDKDKVDKSAVGFEy
Y215-p KSAVGFEyQGKTEKH
T219 GFEyQGKTEKHESQK
Y228 KHESQKDYVKGFGGk
K235-a YVKGFGGkFGVQTDR
K235 YVKGFGGKFGVQTDR
S261 EKLQLHESQKDYKTG
Y265 LHESQKDYKTGFGGK
T267 ESQKDYKTGFGGKFG
K272 YKTGFGGKFGVQSER
K272 YKTGFGGKFGVQSER
S282-p VQSERQDsSAVGFDy
Y289-p sSAVGFDyKERLAKH
K290 SAVGFDyKERLAKHE
S298-p ERLAKHEsQQDyAKG
Y302-p KHEsQQDyAKGFGGk
A303 HEsQQDyAKGFGGkY
K304 EsQQDyAKGFGGkYG
K309 yAKGFGGKYGVQKDR
K309-u yAKGFGGkYGVQKDR
Y310 AKGFGGkYGVQKDRM
K319 VQKDRMDKNASTFEE
S322 DRMDKNASTFEEVVQ
T323 RMDKNASTFEEVVQV
Y334-p VVQVPSAyQkTVPIE
K336-u QVPSAyQkTVPIEAV
S345 VPIEAVTSKTSNIRA
K346 PIEAVTSKTSNIRAN
S348 EAVTSKTSNIRANFE
K399 EEQARAKKQtPPAsP
T401-p QARAKKQtPPAsPsP
S405-p KKQtPPAsPsPQPIE
S407-p QtPPAsPsPQPIEDR
I411 AsPsPQPIEDRPPss
S417-p PIEDRPPssPIyEDA
S418-p IEDRPPssPIyEDAA
Y421-p RPPssPIyEDAAPFK
A424 ssPIyEDAAPFKAEP
P426 PIyEDAAPFKAEPSy
S432 APFKAEPSyRGSEPE
Y433-p PFKAEPSyRGSEPEP
- gap
S436 AEPSyRGSEPEPEyS
Y442-p GSEPEPEySIEAAGI
S443 SEPEPEySIEAAGIP
I449 ySIEAAGIPEAGSQQ
Y460-p GSQQGLTyTSEPVyE
Y466-p TyTSEPVyETTEAPG
Y475-p TTEAPGHyQAEDDTy
T481 HyQAEDDTyDGYESD
Y482-p yQAEDDTyDGYESDL
Y534 RGVCKGRYGLFPANY
Y541 YGLFPANYVELRQ__
4569 : Phospho-Cortactin (Tyr421) Antibody
  rat

 
S5 ___MWKASAGHAVSI
S11 ASAGHAVSITQDDGE
T13 AGHAVSITQDDGEAD
T24 GEADDWETDPDFVND
S33 PDFVNDVSEKEQRWG
T43 EQRWGAKTVQGSGHQ
Y84 GPKASHGYGGKFGVE
K87 ASHGYGGKFGVEQDR
K87 ASHGYGGKFGVEQDR
Y104 KSAVGHEYQSKLsKH
S109-p HEYQSKLsKHCSQVD
S113 SKLsKHCSQVDSVRG
S117 KHCSQVDSVRGFGGK
R119 CSQVDSVRGFGGKFG
K124 SVRGFGGKFGVQMDR
K124 SVRGFGGKFGVQMDR
Y141 QSAVGFEYQGKTEKH
S150 GKTEKHASQKDYSSG
Y154 KHASQKDYSSGFGGK
S155 HASQKDYSSGFGGKY
S156 ASQKDYSSGFGGKYG
K161 YSSGFGGKYGVQADR
K161 YSSGFGGKYGVQADR
Y162 SSGFGGKYGVQADRV
K171 VQADRVDKSAVGFDY
K171 VQADRVDKSAVGFDY
S172 QADRVDKSAVGFDYQ
Y178 KSAVGFDYQGKTEKH
K181 VGFDYQGKTEKHESQ
T182 GFDYQGKTEKHESQK
Y191 KHESQKDYSKGFGGK
K198 YSKGFGGKYGIDKDK
K198 YSKGFGGKYGIDKDK
K203 GGKYGIDKDKVDKSA
K208 IDKDKVDKSAVGFEY
Y215 KSAVGFEYQGKTEKH
T219 GFEYQGKTEKHESQK
Y228 KHESQKDYVKGFGGK
K235 YVKGFGGKFGVQTDR
K235 YVKGFGGKFGVQTDR
S261-p EKLQLHEsQKDYAKG
Y265 LHEsQKDYAKGFGGK
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
A266 HEsQKDYAKGFGGKY
K267 EsQKDYAKGFGGKYG
K272 YAKGFGGKYGVQKDR
K272 YAKGFGGKYGVQKDR
Y273 AKGFGGKYGVQKDRM
K282 VQKDRMDKNASTFEE
S285 DRMDKNASTFEEVVQ
T286 RMDKNASTFEEVVQV
Y297 VVQVPSAYQKTVPIE
K299 QVPSAYQKTVPIEAV
S308 VPIEAVTSKTSNIRA
K309 PIEAVTSKTSNIRAN
S311 EAVTSKTSNIRANFE
K361 EEQARAKKQtPPAsP
T363-p QARAKKQtPPAsPsP
S367-p KKQtPPAsPsPQPAE
S369-p QtPPAsPsPQPAEDR
A373 AsPsPQPAEDRPPsS
S379-p PAEDRPPsSPIyEDt
S380 AEDRPPsSPIyEDtA
Y383-p RPPsSPIyEDtAPLK
T386-p sSPIyEDtAPLKAEP
P388 PIyEDtAPLKAEPSY
S394 APLKAEPSYGSSEPE
Y395 PLKAEPSYGSSEPEP
- gap
S398 AEPSYGSSEPEPEYS
Y404 SSEPEPEYSTEAAGL
S405 SEPEPEYSTEAAGLP
L411 YSTEAAGLPEASNQQ
Y422 SNQQGLAYTSEPVyE
Y428-p AYTSEPVyETTEVPG
Y437 TTEVPGHYQAEDDTy
T443 HYQAEDDTyDGYESD
Y444-p YQAEDDTyDGYESDL
Y496 RGVCKGRYGLFPANY
Y503 YGLFPANYVELRQ__
4569 : Phospho-Cortactin (Tyr421) Antibody
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