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Protein Page:
SLP76 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
SLP76 an adaptor protein involved in signaling through the T cell antigen receptor complex and signaling in other hematopoietic lineages including platelets. Originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation. Platelets deficient in SLP76 do not couple GPVI engagement with the signaling cascade that induces degranulation and aggregation in response to collagen. Contains four domains: a COOH-terminal SH2 domain, a central proline-rich domain, an NH2-terminal sterile {alpha} motif domain, and acidic region that contains three tyrosine phosphorylation motifs: Y112ESP, Y128ESP, and Y145EPP. pY112 and pY128 bind SH2 domains of Vav and Nck. pY145 is recognized by SH2 domains of the Tec family kinases Itk and Btk. Highly expressed in spleen, thymus, peripheral blood leukocytes, mast cells, platelets, and T-cell and monocytic cell lines. Expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Cellular Component: cytosol
Molecular Function: protein binding
Biological Process: platelet activation; immune response; blood coagulation; T cell receptor signaling pathway; mast cell activation; cytokine secretion; transmembrane receptor protein tyrosine kinase signaling pathway
Reference #:  Q13094 (UniProtKB)
Alt. Names/Synonyms: 76 kDa tyrosine phosphoprotein; LCP2; Lymphocyte cytosolic protein 2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa); SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76
Gene Symbols: LCP2
Molecular weight: 60,188 Da
Basal Isoelectric point: 5.89  Predict pI for various phosphorylation states
CST Pathways:  T Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SLP76
Protein Structure Not Found.


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Sites Implicated In
cytoskeletal reorganization: Y113‑p, Y128‑p, Y145‑p
transcription, altered: Y113‑p, Y128‑p, Y145‑p, S376‑p
molecular association, regulation: Y113‑p, Y128‑p, Y145‑p, S376‑p
phosphorylation: Y113‑p, Y128‑p, Y145‑p
protein degradation: S376‑p
ubiquitination: K30‑u, S376‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 Y23 DPDSLADYFKKLNyk
0 6 Y29-p DYFKKLNykDCEKAV
1 1 K30-u YFKKLNykDCEKAVK
0 2 Y39-p CEKAVKKyHIDGARF
0 2 S106-p EEDNGGWssFEEDDy
0 2 S107-p EDNGGWssFEEDDyE
14 0 Y113-p ssFEEDDyESPNDDQ
16 0 Y128-p DGEDDGDyEsPNEEE
1 1 S130-p EDDGDyEsPNEEEEA
14 0 Y145-p PVEDDADyEPPPSND
0 6 S169-p PAKPFPNsNsMyIDR
0 6 S171-p KPFPNsNsMyIDRPP
1 122 Y173-p FPNsNsMyIDRPPSG
1 9 S207-p PPAGRNHsPLPPPQT
0 3 P210 GRNHsPLPPPQTNHE
0 2 S220-p QTNHEEPsRsRNHKt
0 1 S222-p NHEEPsRsRNHKtAK
0 1 T227-p sRsRNHKtAKLPAPS
0 1 S234 tAKLPAPSIDRSTKP
0 1 S246-p TKPPLDRsLAPFDRE
0 1 K260-u EPFTLGKkPPFsDKP
0 1 S264-p LGKkPPFsDKPsIPA
0 3 S268-p PPFsDKPsIPAGRSL
0 2 S339-p PALLPMSsNtFPsRS
0 16 T341-p LLPMSsNtFPsRSTK
0 2 S344-p MSsNtFPsRSTKPSP
0 1 S374-p SNSSFPQsAsLPPYF
3 0 S376-p SSFPQsAsLPPYFSQ
1 4 S410-p PNKPRPPsPAEEENS
0 1 Y423-p NSLNEEWyVsYItRP
0 1 S425-p LNEEWyVsYItRPEA
0 1 T428-p EWyVsYItRPEAEAA
0 1 T454-p VRDSSKKtttNPyVL
0 1 T455-p RDSSKKtttNPyVLM
0 1 T456-p DSSKKtttNPyVLMV
0 13 Y459-p KKtttNPyVLMVLyK
0 1 Y465-p PyVLMVLyKDKVyNI
0 1 Y470-p VLyKDKVyNIQIRyQ
0 1 Y476-p VyNIQIRyQKESQVy
0 2 Y483-p yQKESQVyLLGTGLR
0 5 K492-u LGTGLRGkEDFLSVS
0 8 Y532-p TLTHAAGyP______
  mouse

 
Y23-p NSDNLADyFRKLNyR
Y29-p DyFRKLNyRDCEKAV
R30 yFRKLNyRDCEKAVK
Y39 CEKAVKKYHIDGARF
S106 EEDDGGWSSFEDDyE
S107 EDDGGWSSFEDDyES
Y112-p WSSFEDDyESPNDDD
Y128-p DGEDDGDyESPNEEE
S130 EDDGDyESPNEEEQA
Y145-p LVDDAADyEPPPSNN
T169 PPNSFHNTNSMYIDR
S171 NSFHNTNSMYIDRPP
Y173 FHNTNSMYIDRPPTG
S207-p LPTGRNHsPLsPPHP
S210-p GRNHsPLsPPHPNHE
S220-p HPNHEEPsRSGNNKT
S222 NHEEPsRSGNNKTAK
T227 sRSGNNKTAKLPAPs
S234-p TAKLPAPsIDRSTKP
S246 TKPPLDRSLAPLDRE
K260 EPFILGKKPPFSDKP
S264 LGKKPPFSDKPSAPL
S268 PPFSDKPSAPLGREH
S339 PSLPSMSSNTFPSRS
T341 LPSMSSNTFPSRSVQ
S344 MSSNTFPSRSVQPSS
S374 SNIGFQQSAsLPPYF
S376-p IGFQQSAsLPPYFSQ
S410-p PNRPQPPsPGEEETP
Y423 TPLDEEWYVSYITRP
S425 LDEEWYVSYITRPEA
T428 EWYVSYITRPEAEAA
T454 VRDSSKKTANNPyVL
A455 RDSSKKTANNPyVLM
N456 DSSKKTANNPyVLMV
Y459-p KKTANNPyVLMVLYK
Y465 PyVLMVLYKDKVYNI
Y470 VLYKDKVYNIQIRYQ
Y476 VYNIQIRYQEESQVy
Y483-p YQEESQVyLLGTGLR
K492 LGTGLRGKEDFLSVS
C532 TLTHAAGCL______
  rat

 
Y23 NPDNLADYFRKLNYR
Y29 DYFRKLNYRDCEKAV
R30 YFRKLNYRDCEKAVK
Y39 CEKAVKKYHIDGARF
S106 EEDDGGWSSFEDDYE
S107 EDDGGWSSFEDDYES
Y112 WSSFEDDYESPNDDD
Y128-p DGEDDGDyESPNEEE
S130 EDDGDyESPNEEEET
Y145 LVDDAADYEPPPSND
T169 PAKPFPNTNSMYIDR
S171 KPFPNTNSMYIDRPP
Y173 FPNTNSMYIDRPPTG
S207 LPTGRNHSPNHEEPS
- gap
S214 SPNHEEPSRNRNHKT
N216 NHEEPSRNRNHKTAK
T221 SRNRNHKTAKLPAPS
S228 TAKLPAPSIDRSTKP
S240 TKPPLDRSLAPPLDR
K254 REPLLGKKPSDKPSA
S256 PLLGKKPSDKPSAPL
S260 KKPSDKPSAPLGREH
S331 PSLPSMSSNTFPSRS
T333 LPSMSSNTFPSRSTK
S336 MSSNTFPSRSTKPSP
S366 SNIGFQQSASLPSYF
S368 IGFQQSASLPSYFSQ
S402 PNRPQPPSPGEEESP
Y415 SPLDEEWYVSYITRP
S417 LDEEWYVSYITRPEA
T420 EWYVSYITRPEAEAA
T446 VRDSSKKTVNNPYVL
V447 RDSSKKTVNNPYVLM
N448 DSSKKTVNNPYVLMV
Y451 KKTVNNPYVLMVLYK
Y457 PYVLMVLYKDKVYNI
Y462 VLYKDKVYNIQIRYQ
Y468 VYNIQIRYQEESQVY
Y475 YQEESQVYLLGTGLR
K484 LGTGLRGKEDFLSVS
C524 TLTHAAGCL______
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