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Protein Page:
PFKFB2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PFKFB2 a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Phosphatase (non-protein); Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.46; EC 2.7.1.105; Kinase, other
Cellular Component: cytosol
Molecular Function: protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; protein kinase binding; ATP binding
Biological Process: glycolysis; positive regulation of insulin secretion; glucose metabolic process; fructose 2,6-bisphosphate metabolic process; glucose catabolic process; dephosphorylation; carbohydrate phosphorylation; lactate metabolic process; carbohydrate metabolic process; response to glucose stimulus; pyruvate metabolic process; fructose metabolic process; positive regulation of glucokinase activity
Reference #:  O60825 (UniProtKB)
Alt. Names/Synonyms: 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; DKFZp781D2217; F262; Fructose-2,6-bisphosphatase; fructose-2,6-bisphosphatase, cardiac isozyme; MGC138308; MGC138310; PFK-2/FBPase-2; PFK/FBPase 2; PFKFB, cardiac; PFKFB2
Gene Symbols: PFKFB2
Molecular weight: 58,477 Da
Basal Isoelectric point: 8.61  Predict pI for various phosphorylation states
CST Pathways:  PI3K/Akt Signaling  |  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PFKFB2

Protein Structure Not Found.


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Sites Implicated In
intracellular localization: S32‑p
molecular association, regulation: S32‑p, S483‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
3 0 S32-p EKKCSWAsYMTNSPT
0 1 S175 ILEVKVSSPDYPERN
0 1 K192 NVMEDFLKRIECYKV
0 2 Y356-p ALRDQEKyLyRYPGG
0 2 Y358-p RDQEKyLyRYPGGES
0 2 Y366-p RYPGGESyQDLVQRL
0 6 T458-p NNFPKNQtPVRMRRN
9 343 S466-p PVRMRRNsFtPLssS
0 29 T468-p RMRRNsFtPLssSNt
0 2 S471-p RNsFtPLssSNtIRR
0 1 S472-p NsFtPLssSNtIRRP
2 9 T475-p tPLssSNtIRRPRNy
0 5 Y482-p tIRRPRNysVGsRPL
6 114 S483-p IRRPRNysVGsRPLK
0 10 S486-p PRNysVGsRPLKPLs
1 165 S493-p sRPLKPLsPLRAQDM
1 3 - gap
1 0 - gap
13064 : Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb
  PFKFB2 iso2  
S32 EKKCSWASYMTNSPT
S175 ILEVKVSSPDYPERN
K192 NVMEDFLKRIECYKV
Y356 ALRDQEKYLYRYPGG
Y358 RDQEKYLYRYPGGES
Y366 RYPGGESYQDLVQRL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S463-p LAVRRRPsAASLMLP
- gap
  mouse

► Hide Isoforms
 
S35 GKTCSWASYMTNSPT
S178-p ILEVKVSsPDYPERN
K195-ac NVMEDFLkRIECYKV
Y359 ALRDQEKYLYRYPGG
Y361 RDQEKYLYRYPGGES
Y369 RYPGGESYQDLVQRL
T461-p HNFPKSQtPVRMRRN
S469-p PVRMRRNsFtPLSSS
T471-p RMRRNsFtPLSSSNT
S474 RNsFtPLSSSNTIRR
S475 NsFtPLSSSNTIRRP
T478 tPLSSSNTIRRPRNY
Y485 TIRRPRNYsVGsRPL
S486-p IRRPRNYsVGsRPLK
S489-p PRNYsVGsRPLKPLs
S496-p sRPLKPLsPLRALDM
- gap
- gap
  PFKFB2 iso3  
S34 GKTCSWASYMTNSPT
S177 ILEVKVSSPDYPERN
K194 NVMEDFLKRIECYKV
Y358 ALRDQEKYLYRYPGG
Y360 RDQEKYLYRYPGGES
Y368 RYPGGESYQDLVQRL
T460 HNFPKSQTPVRMRRN
S468 PVRMRRNSFTPLSSS
T470 RMRRNSFTPLSSSNT
S473 RNSFTPLSSSNTIRR
S474 NSFTPLSSSNTIRRP
T477 TPLSSSNTIRRPRNY
Y484 TIRRPRNYsVGsRPL
S485-p IRRPRNYsVGsRPLK
S488-p PRNYsVGsRPLKPLs
S495-p sRPLKPLsPLRALDM
- gap
- gap
  rat

 
S35 GKKCSWASYMTNSPT
S178 ILEVKVSSPDYPERN
K195 NVMEDFLKRIECYKV
Y359 ALRDQEKYLYRYPGG
Y361 RDQEKYLYRYPGGES
Y369 RYPGGESYQDLVQRL
T461 HNFPKSQTPVRMRRN
S469-p PVRMRRNsFtPLSSS
T471-p RMRRNsFtPLSSSNT
S474 RNsFtPLSSSNTIRR
S475 NsFtPLSSSNTIRRP
T478 tPLSSSNTIRRPRNY
Y485 TIRRPRNYsVGSRPL
S486-p IRRPRNYsVGSRPLK
S489 PRNYsVGSRPLKPLs
S496-p SRPLKPLsPLRALDM
S549 LAKHRRPSMASLTLL
- gap
  cow

 
S33 EKKCSWASYMTNSPT
S176 ILEVKVSSPDYPERN
K193 NVMDDFLKRIECYKV
Y357 ALRDEEKYLYRYPGG
Y359 RDEEKYLYRYPGGES
Y367 RYPGGESYQDLVQRL
T459 NNFPKSQTPVRMRRN
S467-p PVRMRRNsFTPLSSS
T469 RMRRNsFTPLSSSNt
S472 RNsFTPLSSSNtIRR
S473 NsFTPLSSSNtIRRP
T476-p TPLSSSNtIRRPRNY
Y483 tIRRPRNYsVGSRPL
S484-p IRRPRNYsVGSRPLQ
S487 PRNYsVGSRPLQPLs
S494-p SRPLQPLsPLRALDT
S523-p RAAHRLPsPAPPTsP
S529-p PsPAPPTsPS_____
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