Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
PFKFB2 (human)

Overview
PFKFB2 a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: EC 3.1.3.46; Phosphatase (non-protein); EC 2.7.1.105; Kinase, other; Carbohydrate Metabolism - fructose and mannose; Motility/polarity/chemotaxis
Cellular Component: cytosol
Molecular Function: protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; protein kinase binding; ATP binding
Biological Process: glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; lactate metabolic process; response to glucose stimulus; positive regulation of insulin secretion; glucose metabolic process; pyruvate metabolic process; fructose 2,6-bisphosphate metabolic process; positive regulation of glucokinase activity; fructose metabolic process
Reference #:  O60825 (UniProtKB)
Alt. Names/Synonyms: 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; DKFZp781D2217; F262; Fructose-2,6-bisphosphatase; fructose-2,6-bisphosphatase, cardiac isozyme; MGC138308; MGC138310; PFK-2/FBPase-2; PFK/FBPase 2; PFKFB, cardiac; PFKFB2
Gene Symbols: PFKFB2
Molecular weight: 58,477 Da
Basal Isoelectric point: 8.61  Predict pI for various phosphorylation states
CST Pathways:  PI3K/Akt Signaling  |  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PFKFB2

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  ENZYME  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
molecular association, regulation: S483‑p

Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
2 0 S32-p EKKCSWAsYMTNSPT
0 1 S175 ILEVKVSSPDYPERN
0 2 Y356-p ALRDQEKyLyRYPGG
0 2 Y358-p RDQEKyLyRYPGGES
0 3 Y366-p RYPGGESyQDLVQRL
0 6 T458-p NNFPKNQtPVRMRRN
9 340 S466-p PVRMRRNsFtPLssS
0 28 T468-p RMRRNsFtPLssSNt
0 2 S471-p RNsFtPLssSNtIRR
0 1 S472-p NsFtPLssSNtIRRP
2 9 T475-p tPLssSNtIRRPRNy
0 5 Y482-p tIRRPRNysVGsRPL
6 111 S483-p IRRPRNysVGsRPLK
0 9 S486-p PRNysVGsRPLKPLs
1 163 S493-p sRPLKPLsPLRAQDM
1 3 - gap
1 0 - gap
13064 : Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb
  PFKFB2 iso2  
S32 EKKCSWASYMTNSPT
S175 ILEVKVSSPDYPERN
Y356 ALRDQEKYLYRYPGG
Y358 RDQEKYLYRYPGGES
Y366 RYPGGESYQDLVQRL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S463-p LAVRRRPsAASLMLP
- gap
  mouse

► Hide Isoforms
 
S35 GKTCSWASYMTNSPT
S178-p ILEVKVSsPDYPERN
Y359 ALRDQEKYLYRYPGG
Y361 RDQEKYLYRYPGGES
Y369 RYPGGESYQDLVQRL
T461-p HNFPKSQtPVRMRRN
S469-p PVRMRRNsFtPLSSS
T471-p RMRRNsFtPLSSSNT
S474 RNsFtPLSSSNTIRR
S475 NsFtPLSSSNTIRRP
T478 tPLSSSNTIRRPRNY
Y485 TIRRPRNYsVGsRPL
S486-p IRRPRNYsVGsRPLK
S489-p PRNYsVGsRPLKPLs
S496-p sRPLKPLsPLRALDM
- gap
- gap
  PFKFB2 iso3  
S34 GKTCSWASYMTNSPT
S177 ILEVKVSSPDYPERN
Y358 ALRDQEKYLYRYPGG
Y360 RDQEKYLYRYPGGES
Y368 RYPGGESYQDLVQRL
T460 HNFPKSQTPVRMRRN
S468 PVRMRRNSFTPLSSS
T470 RMRRNSFTPLSSSNT
S473 RNSFTPLSSSNTIRR
S474 NSFTPLSSSNTIRRP
T477 TPLSSSNTIRRPRNY
Y484 TIRRPRNYsVGsRPL
S485-p IRRPRNYsVGsRPLK
S488-p PRNYsVGsRPLKPLs
S495-p sRPLKPLsPLRALDM
- gap
- gap
  rat

 
S35 GKKCSWASYMTNSPT
S178 ILEVKVSSPDYPERN
Y359 ALRDQEKYLYRYPGG
Y361 RDQEKYLYRYPGGES
Y369 RYPGGESYQDLVQRL
T461 HNFPKSQTPVRMRRN
S469-p PVRMRRNsFtPLSSS
T471-p RMRRNsFtPLSSSNT
S474 RNsFtPLSSSNTIRR
S475 NsFtPLSSSNTIRRP
T478 tPLSSSNTIRRPRNY
Y485 TIRRPRNYsVGSRPL
S486-p IRRPRNYsVGSRPLK
S489 PRNYsVGSRPLKPLs
S496-p SRPLKPLsPLRALDM
S549 LAKHRRPSMASLTLL
- gap
  cow

 
S33 EKKCSWASYMTNSPT
S176 ILEVKVSSPDYPERN
Y357 ALRDEEKYLYRYPGG
Y359 RDEEKYLYRYPGGES
Y367 RYPGGESYQDLVQRL
T459 NNFPKSQTPVRMRRN
S467-p PVRMRRNsFTPLSSS
T469 RMRRNsFTPLSSSNt
S472 RNsFTPLSSSNtIRR
S473 NsFTPLSSSNtIRRP
T476-p TPLSSSNtIRRPRNY
Y483 tIRRPRNYsVGSRPL
S484-p IRRPRNYsVGSRPLQ
S487 PRNYsVGSRPLQPLs
S494-p SRPLQPLsPLRALDT
S523-p RAAHRLPsPAPPTsP
S529-p PsPAPPTsPS_____
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.