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Protein Page:
BAD (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BAD a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. Note: This description may include information from UniProtKB.
Protein type: Apoptosis
Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol
Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; lipid binding; protein kinase binding; protein phosphatase 2B binding
Biological Process: response to oleate; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; apoptosis; positive regulation of apoptosis; response to glucocorticoid stimulus; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; response to estradiol stimulus; positive regulation of apoptosis by virus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; epidermal growth factor receptor signaling pathway; response to drug; caspase activation; release of cytochrome c from mitochondria; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; positive regulation of T cell differentiation; induction of apoptosis via death domain receptors; positive regulation of B cell differentiation; innate immune response; regulation of mitochondrial membrane permeability; response to calcium ion; response to progesterone stimulus; positive regulation of epithelial cell proliferation
Reference #:  Q92934 (UniProtKB)
Alt. Names/Synonyms: BAD; BBC2; BBC6; Bcl-2-binding component 6; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; BCL2-binding component 6; BCL2-binding protein; Bcl2-L-8; BCL2L8
Gene Symbols: BAD
Molecular weight: 18,392 Da
Basal Isoelectric point: 6.6  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  ErbB/HER Signaling  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Mitochondrial Control of Apoptosis  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BAD

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S75‑p, S99‑p, S118‑p
apoptosis, inhibited: S75‑p, S99‑p, S118‑p
cell adhesion, altered: S75‑p
cell growth, altered: S75‑p, S99‑p
transcription, altered: S75‑p
transcription, inhibited: S75‑p, S99‑p
activity, induced: S75‑p, S99‑p, T137‑p
activity, inhibited: S75‑p
molecular association, regulation: S74‑p, S75‑p, S99‑p, S118‑p
phosphorylation: S74‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 - gap
0 5 S25-p AERGLGPsPAGDGPs
0 2 S32-p sPAGDGPsGsGkHHR
0 4 S34-p AGDGPsGsGkHHRQA
0 1 K36-ac DGPsGsGkHHRQAPG
1 0 S57 HQQEQPTSSSHHGGA
1 0 S59 QEQPTSSSHHGGAGA
0 29 S71-p AGAVEIRsRHssyPA
1 76 S74-p VEIRsRHssyPAGtE
112 179 S75-p EIRsRHssyPAGtED
0 49 Y76-p IRsRHssyPAGtEDD
1 28 T80-p HssyPAGtEDDEGMG
7 44 S91-p EGMGEEPsPFRGRsR
1 20 S97-p PsPFRGRsRsAPPNL
111 239 S99-p PFRGRsRsAPPNLWA
0 1 Y110-p NLWAAQRyGRELRRM
43 192 S118-p GRELRRMsDEFVDsF
1 1 S124-p MsDEFVDsFKKGLPR
6 13 S134-p KGLPRPKsAGtAtQM
2 0 T137-p PRPKsAGtAtQMRQS
0 1 T139-p PKsAGtAtQMRQSSs
0 1 S146-p tQMRQSSsWTRVFQS
0 5 R161-m1 WWDRNLGrGSSAPSQ
3 0 A165 NLGrGSSAPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  mouse

 
S26-p KSDPGIRsLGSDAGG
S67 TDRGLGPSLTEDQPG
G74 SLTEDQPGPyLAPGL
Y76-p TEDQPGPyLAPGLLG
- gap
T94-p HQQGRAAtNsHHGGA
S96-p QGRAAtNsHHGGAGA
S108-p AGAMETRsRHssyPA
S111-p METRsRHssyPAGtE
S112-p ETRsRHssyPAGtEE
Y113-p TRsRHssyPAGtEED
T117-p HssyPAGtEEDEGME
S128-p EGMEEELsPFRGRsR
S134-p LsPFRGRsRsAPPNL
S136-p PFRGRsRsAPPNLWA
Y147 NLWAAQRYGRELRRM
S155-p GRELRRMsDEFEGSF
S161 MsDEFEGSFKGLPRP
S170-p KGLPRPKsAGtATQM
T173-p PRPKsAGtATQMRQS
T175 PKsAGtATQMRQSAG
G182 TQMRQSAGWTRIIQS
K197 WWDRNLGKGGStPSQ
T201-p NLGKGGStPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  rat

 
S26 KSDPGIRSLGSDAGG
S67 TDRGLGPSLTEDQPG
G74 SLTEDQPGPYLAPGL
Y76 TEDQPGPYLAPGLLG
- gap
N95 QQPGQAANNSHHGGA
S97 PGQAANNSHHGGAGT
S109 AGTMETRSRHSsYPA
S112 METRSRHSsYPAGtE
S113-p ETRSRHSsYPAGtEE
Y114 TRSRHSsYPAGtEED
T118-p HSsYPAGtEEDEGME
S129-p EGMEEELsPFRGRSR
S135 LsPFRGRSRsAPPNL
S137-p PFRGRSRsAPPNLWA
Y148 NLWAAQRYGRELRRM
S156-p GRELRRMsDEFEGSF
S162 MsDEFEGSFKGLPRP
S171 KGLPRPKSAGTATQM
T174 PRPKSAGTATQMRQS
T176 PKSAGTATQMRQSAS
S183 TQMRQSASWTRIIQS
K198 WWDRNLGKGGSTPSQ
T202 NLGKGGSTPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
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