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PAK1 (mouse)

Overview PAK1 a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK. Note: This description may include information from UniProtKB. Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; PAKA subfamily Cellular Component: Golgi apparatus; nuclear membrane; focal adhesion; protein complex; dendrite; intercellular junction; cytosol; Z disc; ruffle; growth cone; membrane; axon; cytoplasm; plasma membrane; nucleus Molecular Function: collagen binding; transferase activity; protein serine/threonine kinase activity; nucleotide binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity Biological Process: positive regulation of JNK activity; wound healing; protein amino acid autophosphorylation; cellular response to stress; positive regulation of estrogen receptor signaling pathway; protein amino acid phosphorylation; receptor clustering; positive regulation of peptidyl-serine phosphorylation; response to organic substance; branching morphogenesis of a tube; cellular response to insulin stimulus; regulation of actin cytoskeleton organization and biogenesis; positive regulation of stress fiber formation; actin cytoskeleton reorganization; dendrite development; response to hypoxia; phosphorylation; neurite morphogenesis; neuromuscular junction development Reference #:  O88643 (UniProtKB) Alt. Names/Synonyms: activated protein kinase alpha; Alpha-PAK; AW045634; CDC42/RAC effector kinase PAK-A; OTTMUSP00000023295; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p65-PAK; PAK-1; Pak1; Paka; Serine/threonine-protein kinase PAK 1 Gene Symbols: Pak1 Molecular weight: 60,737 Da Basal Isoelectric point: 5.53  Predict pI for various phosphorylation states CST Pathways:  ErbB/HER Signaling  |  MAPK/Erk in Growth and Differentiation  |  Regulation of Actin Dynamics  |  Regulation of Microtubule Dynamics  |  TGF-ß Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PAK1 1F3M - A/B=70-149, C/D=249-545 (human) 1YHV - A=249-545 (human) 1YHW - A=249-545 (human) 1ZSG - B=183-204 (human) 2HY8 - 1=249-545 (human) 2QME - I=74-109 (human) 3DVP - D/C=212-221 (human) 3FXZ - A=249-545 (human) 3FY0 - A=249-545 (human) 3Q4Z - A/B=248-545 (human) 3Q52 - A=248-545 (human) 3Q53 - A=248-545 (human) 4DAW - A=249-545 (human) 1E0A - B=75-118 (rat) Substrate Sequence Logo

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	Sites Implicated In
cell motility, altered: T212‑p cytoskeletal reorganization: T212‑p activation: S21‑p enzymatic activity, induced: T423‑p enzymatic activity, inhibited: T109‑p intracellular localization: S21‑p, T212‑p molecular association, regulation: S21‑p, T212‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			mouse
0	1		T20	PAPPMRNTsTMIGAG
3	10		S21-p	APPMRNTsTMIGAGS
0	1		K29	TMIGAGSKDTGTLNH
1	0		T84	LPSDFEHTIHVGFDA
1	1		T109-p	QWARLLQtSNITKsE
0	3		S115-p	QtSNITKsEQKKNPQ
0	7		Y131	VLDVLEFYNSKKTSN
0	2		S133	DVLEFYNSKKTSNSK
0	2		T136	EFYNSKKTSNSKKYM
0	2		S137	FYNSKKTSNSKKYMs
0	4		S139	NSKKTSNSKKYMsFT
0	13		Y142	KTSNSKKYMsFTDKS
7	137		S144-p	SNSKKYMsFTDKSAE
0	15		T146	SKKYMsFTDKSAEDY
1	25		S149	YMsFTDKSAEDYNSS
4	23		Y153	TDKSAEDYNSSNtLN
0	4		S155	KSAEDYNSSNtLNVK
0	1		S156	SAEDYNSSNtLNVKT
0	1		T158-p	EDYNSSNtLNVKTVS
1	9		S174-p	TPAVPPVsEDDEDDD
0	6		T185-p	EDDDDDAtPPPVIAP
12	0		S199-p	PRPEHTKsVYTRsVI
4	0		Y201	PEHTKsVYTRsVIEP
10	6		S204-p	TKsVYTRsVIEPLPV
8	28		T212-p	VIEPLPVtPtRDVAt
0	4		T214-p	EPLPVtPtRDVAtsP
0	2		T219-p	tPtRDVAtsPIsPtE
0	9		S220-p	PtRDVAtsPIsPtEN
0	18		S223-p	DVAtsPIsPtENNtt
0	10		T225-p	AtsPIsPtENNttPP
0	8		T229-p	IsPtENNttPPDALT
0	30		T230-p	sPtENNttPPDALTR
0	2		T236	ttPPDALTRNTEKQK
0	1		K256	SDEEILEKLRSIVSV
0	1		K256	SDEEILEKLRSIVSV
4	2		Y285	QGASGTVYTAMDVAT
0	1		K420	QITPEQSKRstMVGt
0	10		K420	QITPEQSKRstMVGt
0	3		S422-p	TPEQSKRstMVGtPY
22	10		T423-p	PEQSKRstMVGtPYW
0	3		T427-p	KRstMVGtPYWMAPE
0	9		Y474	ENPLRALYLIATNGT
0	1		S491	LQNPEKLSAIFRDFL
0	1		K513	VEKRGSAKELLQHQF
0	1		S528	LKIAKPLSSLTPLMH
0	1		S529	KIAKPLSSLTPLMHA
0	1		T531	AKPLSSLTPLMHAAK

	human
T20-p	PAPPMRNtsTMIGAG
S21-p	APPMRNtsTMIGAGS
K29-a	TMIGAGSkDAGTLNH
T84	LPSDFEHTIHVGFDA
T109-p	QWARLLQtSNITKsE
S115-p	QtSNITKsEQKKNPQ
Y131-p	VLDVLEFyNsKKtsN
S133-p	DVLEFyNsKKtsNsQ
T136-p	EFyNsKKtsNsQKyM
S137-p	FyNsKKtsNsQKyMs
S139-p	NsKKtsNsQKyMsFt
Y142-p	KtsNsQKyMsFtDKs
S144-p	sNsQKyMsFtDKsAE
T146-p	sQKyMsFtDKsAEDy
S149-p	yMsFtDKsAEDyNss
Y153-p	tDKsAEDyNssNALN
S155-p	KsAEDyNssNALNVK
S156-p	sAEDyNssNALNVKA
A158	EDyNssNALNVKAVS
S174-p	TPAVPPVsEDEDDDD
T185-p	DDDDDDAtPPPVIAP
S199-p	PRPEHTKsVyTRsVI
Y201-p	PEHTKsVyTRsVIEP
S204-p	TKsVyTRsVIEPLPV
T212-p	VIEPLPVtPtRDVAt
T214-p	EPLPVtPtRDVAtsP
T219-p	tPtRDVAtsPIsPtE
S220-p	PtRDVAtsPIsPtEN
S223-p	DVAtsPIsPtENNtt
T225-p	AtsPIsPtENNttPP
T229-p	IsPtENNttPPDALt
T230-p	sPtENNttPPDALtR
T236-p	ttPPDALtRNTEKQK
K256-a	SDEEILEkLRSIVSV
K256-u	SDEEILEkLRSIVSV
Y285-p	QGASGTVyTAMDVAT
K420-a	QITPEQSkRstMVGt
K420-u	QITPEQSkRstMVGt
S422-p	TPEQSkRstMVGtPY
T423-p	PEQSkRstMVGtPYW
T427-p	kRstMVGtPYWMAPE
Y474-p	ENPLRALyLIATNGT
S491-p	LQNPEKLsAIFRDFL
K513-a	VEKRGSAkELLQHQF
S528-p	LKIAKPLssLtPLIA
S529-p	KIAKPLssLtPLIAA
T531-p	AKPLssLtPLIAAAK

	rat
T20	PAPPMRNTsTMIGAG
S21-p	APPMRNTsTMIGAGS
K29	TMIGAGSKDPGTLNH
T84-p	LPSDFEHtIHVGFDA
T109	QWARLLQTSNITKSE
S115	QTSNITKSEQKKNPQ
Y131	VLDVLEFYNSKKTSN
S133	DVLEFYNSKKTSNSQ
T136	EFYNSKKTSNSQKYM
S137	FYNSKKTSNSQKYMs
S139	NSKKTSNSQKYMsFT
Y142	KTSNSQKYMsFTDKs
S144-p	SNSQKYMsFTDKsAE
T146	SQKYMsFTDKsAEDY
S149-p	YMsFTDKsAEDYNSS
Y153	TDKsAEDYNSSNTLN
S155	KsAEDYNSSNTLNVK
S156	sAEDYNSSNTLNVKT
T158	EDYNSSNTLNVKTVS
S174	TPAVPPVSEDEDDDD
T184	EDDDDDATPPPVIAP
S198-p	PRPEHTKsVYTRsVI
Y200	PEHTKsVYTRsVIEP
S203-p	TKsVYTRsVIEPLPV
T211-p	VIEPLPVtPTRDVAT
T213	EPLPVtPTRDVATSP
T218	tPTRDVATSPISPTE
S219	PTRDVATSPISPTEN
S222	DVATSPISPTENNTT
T224	ATSPISPTENNTTPP
T228	ISPTENNTTPPDALT
T229	SPTENNTTPPDALTR
T235	TTPPDALTRNTEKQK
K255	SDEEILEKLRSIVSV
K255	SDEEILEKLRSIVSV
Y284	QGASGTVYTAMDVAT
K419	QITPEQSKRstMVGT
K419	QITPEQSKRstMVGT
S421-p	TPEQSKRstMVGTPY
T422-p	PEQSKRstMVGTPYW
T426	KRstMVGTPYWMAPE
Y473	ENPLRALYLIATNGT
S490	LQNPEKLSAIFRDFL
K512	VEKRGSAKELLQHQF
S527	LKIAKPLSSLTPLIA
S528	KIAKPLSSLTPLIAA
T530	AKPLSSLTPLIAAAK

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