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Protein Page:
PAK1 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PAK1 a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKA subfamily
Cellular Component: nuclear membrane; focal adhesion; protein complex; dendrite; intercellular junction; Z disc; cytosol; ruffle; cell projection; growth cone; membrane; axon; cytoplasm; plasma membrane; intracellular; nucleus; cell junction
Molecular Function: transferase activity; collagen binding; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; catalytic activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity
Biological Process: positive regulation of JNK activity; exocytosis; wound healing; metabolic process; apoptosis; protein amino acid autophosphorylation; positive regulation of estrogen receptor signaling pathway; receptor clustering; protein amino acid phosphorylation; response to organic substance; positive regulation of stress fiber formation; dendrite development; response to stress; neuromuscular junction development; positive regulation of peptidyl-serine phosphorylation; cellular response to insulin stimulus; branching morphogenesis of a tube; axonogenesis; regulation of actin cytoskeleton organization and biogenesis; actin cytoskeleton reorganization; response to hypoxia; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; phosphorylation; neurite morphogenesis
Reference #:  O88643 (UniProtKB)
Alt. Names/Synonyms: activated protein kinase alpha; Alpha-PAK; AW045634; CDC42/RAC effector kinase PAK-A; OTTMUSP00000023295; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p65-PAK; PAK-1; Pak1; Paka; Serine/threonine-protein kinase PAK 1
Gene Symbols: Pak1
Molecular weight: 60,737 Da
Basal Isoelectric point: 5.53  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  ErbB/HER Signaling  |  Growth And Differentiation Control by MAPKs  |  Microtubule Dynamics  |  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PAK1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell motility, altered: T212‑p
cytoskeletal reorganization: T212‑p
activity, induced: S21‑p
enzymatic activity, induced: T423‑p
enzymatic activity, inhibited: T109‑p
intracellular localization: S21‑p, T212‑p
molecular association, regulation: S21‑p, T212‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
0 1 T20 PAPPMRNTsTMIGAG
3 10 S21-p APPMRNTsTMIGAGS
0 2 K29 TMIGAGSKDTGTLNH
1 0 T84 LPSDFEHTIHVGFDA
1 1 T109-p QWARLLQtSNITKsE
0 1 S110 WARLLQtSNITKsEQ
0 5 S115-p QtSNITKsEQKKNPQ
0 8 Y131 VLDVLEFYNSKKTSN
0 2 S133 DVLEFYNSKKTSNSK
0 5 T136 EFYNSKKTSNSKKYM
0 2 S137 FYNSKKTSNSKKYMs
0 6 S139 NSKKTSNSKKYMsFT
0 17 Y142 KTSNSKKYMsFTDKS
8 155 S144-p SNSKKYMsFTDKSAE
0 15 T146 SKKYMsFTDKSAEDY
1 32 S149 YMsFTDKSAEDYNSS
4 22 Y153 TDKSAEDYNSSNtLN
0 5 S155 KSAEDYNSSNtLNVK
0 1 S156 SAEDYNSSNtLNVKT
0 1 T158-p EDYNSSNtLNVKTVS
1 14 S174-p TPAVPPVsEDDEDDD
0 7 T185-p EDDDDDAtPPPVIAP
0 1 T197 IAPRPEHTKsVYTRs
12 0 S199-p PRPEHTKsVYTRsVI
4 0 Y201 PEHTKsVYTRsVIEP
10 7 S204-p TKsVYTRsVIEPLPV
9 30 T212-p VIEPLPVtPtRDVAt
0 5 T214-p EPLPVtPtRDVAtsP
0 4 T219-p tPtRDVAtsPIsPtE
0 11 S220-p PtRDVAtsPIsPtEN
1 21 S223-p DVAtsPIsPtENNtt
0 12 T225-p AtsPIsPtENNttPP
0 9 T229-p IsPtENNttPPDALT
0 33 T230-p sPtENNttPPDALTR
0 2 T236 ttPPDALTRNTEKQK
0 1 S249-p QKKKPKMsDEEILEK
0 1 K256 sDEEILEKLRsIVsV
0 1 K256 sDEEILEKLRsIVsV
0 1 S259-p EILEKLRsIVsVGDP
0 2 S262-p EKLRsIVsVGDPKKK
4 2 Y285 QGASGTVYTAMDVAT
0 1 K420 QITPEQSKRstMVGt
0 10 K420 QITPEQSKRstMVGt
0 3 S422-p TPEQSKRstMVGtPY
22 11 T423-p PEQSKRstMVGtPYW
0 4 T427-p KRstMVGtPYWMAPE
0 9 Y474 ENPLRALYLIATNGT
0 1 S491 LQNPEKLSAIFRDFL
0 1 K513 VEKRGSAKELLQHQF
0 1 S528 LKIAKPLSSLTPLMH
0 1 S529 KIAKPLSSLTPLMHA
0 1 T531 AKPLSSLTPLMHAAK
2606 : Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2601 : Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
  human

 
T20-p PAPPMRNtsTMIGAG
S21-p APPMRNtsTMIGAGS
K29-ac TMIGAGSkDAGTLNH
T84 LPSDFEHTIHVGFDA
T109-p QWARLLQtsNITKsE
S110-p WARLLQtsNITKsEQ
S115-p QtsNITKsEQKKNPQ
Y131-p VLDVLEFyNsKKtsN
S133-p DVLEFyNsKKtsNsQ
T136-p EFyNsKKtsNsQKyM
S137-p FyNsKKtsNsQKyMs
S139-p NsKKtsNsQKyMsFt
Y142-p KtsNsQKyMsFtDKs
S144-p sNsQKyMsFtDKsAE
T146-p sQKyMsFtDKsAEDy
S149-p yMsFtDKsAEDyNss
Y153-p tDKsAEDyNssNALN
S155-p KsAEDyNssNALNVK
S156-p sAEDyNssNALNVKA
A158 EDyNssNALNVKAVS
S174-p TPAVPPVsEDEDDDD
T185-p DDDDDDAtPPPVIAP
T197-p IAPRPEHtKsVyTRs
S199-p PRPEHtKsVyTRsVI
Y201-p PEHtKsVyTRsVIEP
S204-p tKsVyTRsVIEPLPV
T212-p VIEPLPVtPtRDVAt
T214-p EPLPVtPtRDVAtsP
T219-p tPtRDVAtsPIsPtE
S220-p PtRDVAtsPIsPtEN
S223-p DVAtsPIsPtENNtt
T225-p AtsPIsPtENNttPP
T229-p IsPtENNttPPDALt
T230-p sPtENNttPPDALtR
T236-p ttPPDALtRNTEKQK
S249-p QKKKPKMsDEEILEk
K256-ac sDEEILEkLRsIVsV
K256-ub sDEEILEkLRsIVsV
S259-p EILEkLRsIVsVGDP
S262-p EkLRsIVsVGDPKKK
Y285-p QGASGTVyTAMDVAT
K420-ac QITPEQSkRstMVGt
K420-ub QITPEQSkRstMVGt
S422-p TPEQSkRstMVGtPY
T423-p PEQSkRstMVGtPYW
T427-p kRstMVGtPYWMAPE
Y474-p ENPLRALyLIATNGT
S491-p LQNPEKLsAIFRDFL
K513-ac VEKRGSAkELLQHQF
S528-p LKIAKPLssLtPLIA
S529-p KIAKPLssLtPLIAA
T531-p AKPLssLtPLIAAAK
2606 : Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2605 : Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
2601 : Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
  rat

 
T20 PAPPMRNTsTMIGAG
S21-p APPMRNTsTMIGAGS
K29 TMIGAGSKDPGTLNH
T84-p LPSDFEHtIHVGFDA
T109 QWARLLQTSNITKSE
S110 WARLLQTSNITKSEQ
S115 QTSNITKSEQKKNPQ
Y131 VLDVLEFYNSKKTSN
S133 DVLEFYNSKKTSNSQ
T136 EFYNSKKTSNSQKYM
S137 FYNSKKTSNSQKYMs
S139 NSKKTSNSQKYMsFT
Y142 KTSNSQKYMsFTDKs
S144-p SNSQKYMsFTDKsAE
T146 SQKYMsFTDKsAEDY
S149-p YMsFTDKsAEDYNSS
Y153 TDKsAEDYNSSNTLN
S155 KsAEDYNSSNTLNVK
S156 sAEDYNSSNTLNVKT
T158 EDYNSSNTLNVKTVS
S174-p TPAVPPVsEDEDDDD
T184 EDDDDDATPPPVIAP
T196 IAPRPEHTKsVYTRs
S198-p PRPEHTKsVYTRsVI
Y200 PEHTKsVYTRsVIEP
S203-p TKsVYTRsVIEPLPV
T211-p VIEPLPVtPTRDVAT
T213 EPLPVtPTRDVATSP
T218 tPTRDVATSPISPTE
S219 PTRDVATSPISPTEN
S222 DVATSPISPTENNTT
T224 ATSPISPTENNTTPP
T228 ISPTENNTTPPDALT
T229 SPTENNTTPPDALTR
T235 TTPPDALTRNTEKQK
S248 QKKKPKMSDEEILEK
K255 SDEEILEKLRSIVSV
K255 SDEEILEKLRSIVSV
S258 EILEKLRSIVSVGDP
S261 EKLRSIVSVGDPKKK
Y284 QGASGTVYTAMDVAT
K419 QITPEQSKRstMVGT
K419 QITPEQSKRstMVGT
S421-p TPEQSKRstMVGTPY
T422-p PEQSKRstMVGTPYW
T426 KRstMVGTPYWMAPE
Y473 ENPLRALYLIATNGT
S490 LQNPEKLSAIFRDFL
K512 VEKRGSAKELLQHQF
S527 LKIAKPLSSLTPLIA
S528 KIAKPLSSLTPLIAA
T530 AKPLSSLTPLIAAAK
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